LOCUS       sequence-475999-       12833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one plant virus expression vector for virus-induced gene 
            silencing (VIGS) and virus-mediated overexpression (VOX).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12833)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 12833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVSe
FEATURES             Location/Qualifiers
     source          1..12833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(5919..5936)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5919..5935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5928..5950)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    6138..6162
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7463..8092
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8521..9594
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9660..9854
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10090..10112)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10198..10338
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10253..10272
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10353..10370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10524..11112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10604..10623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11199..11993)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12418..12442
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    12710..12731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12746..12751,12752..12769,12770..12776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12784..12800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12789..12811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12808..12824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12808..12824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtata ggaaaaaata tttattattt
      961 tttaaattta acctattgaa aatgtacagt ttaaagagta gacgtggatc gaattacatt
     1021 caagcacgtg gaaactcacg gaccccatta ttcaaacgac attccactgg taagcgttat
     1081 ggaggaagac gactacagcc actgaatgtg aaaagagaag atgaacccag aatgtcgacc
     1141 caacgcatcc acgagaatca atatgggtcg gaatttacaa tggcccacaa cacagccatt
     1201 tcgacctata tctcctatcc taatctgggc aagactgaac ccaatcgaac caggtcgtac
     1261 atcaagctga aacgattgcg tttcaagggc acggtgaaga tcgaacgagt tcctactgac
     1321 gtggctatgg atggttcgat tcccaacacg gaaggggtgt tctcattggt catagtcgtt
     1381 gaccgaaaac ctcatttgac tgcatctgga tgcctacata cgtttgacga actatttggt
     1441 gcgataatcc acagccatgg aaccctgtct atagttcccg cgttgaagga tcgttactac
     1501 atacggcatg taattaaacg ggtactgtcc gtcgagaaag ataacgtcat ggtggatatt
     1561 gaaggatcca ctgctctgtc taataggcgt tttaattgtt gggccagttt taaagatctt
     1621 gatttggatt catgtaaggg tgtttatgac aacataaaca agaatgccct gttagtttat
     1681 tactcatggg tatcggatac tgtgtctaaa gcatccactt atgtatcgta tgaccttgat
     1741 tatattggat aatcaataat aacaggaaaa tttgaataag aaaaaatatt atttaaatat
     1801 ttttggttga ggaccattac aactactctt gatacattct tggaccgttg tcctaaccag
     1861 ttcgtttaat tgggccatag acatggtaat gttggactca gccctatggg cccctactat
     1921 tgatgccgag tcacctggat ccagtacagt ggaccctaac ctgtgcagct ctctgtaagg
     1981 tgtctgttcg ttattggact gtgagtccgc atcttcgtta acgggcccta tggtactcct
     2041 ggtcgcccac gactcccctg gccttatttc tattgggcct ggaagcccaa atctgttcat
     2101 ggatgcggac ctaattagtt tcctttccca tttaccatag tctacatgtg agaaatcaac
     2161 gtctttagcg gagaactgtt tggaaaggat ctttaccgtt ggcgccttga acggaatatc
     2221 gacggaatgt ttggcggtcg ataatttcag cttccctttg aattttgcga agtgggtacg
     2281 ctgttgaaca ttcgtatcag aaactctgta atagagtttc caagggattg ggtctttgag
     2341 agagaagaag gaagctgaaa aatagtggag atctatgtta cacctgatcg gaaaagtcca
     2401 cgacgattgt aatgattcat tatcagtcat cctcttgtcg tgaatctcca ctatcactgt
     2461 accagtcgcg ttaattggca cctgctgcct gtactcgata acgcaatgat cgatcttcat
     2521 gcaactgcga ctgagtctcg ccgttaattg tgccgccgtt gacggaaact gcagaacgat
     2581 ctcagttagg tcatgggaaa gctgatattc atccctctga gactctatat aattaaatgc
     2641 gtttggggga ttagctaatt gagaatccat atagagaaat atggccgcgc agcgacgccg
     2701 gttgaagaat atgaacaagg aataagatga acaaggaaga agatgaacaa gcaataattt
     2761 aattaattat tgttaataag cataaattat gaaaataatt ttgatggtaa tgaaagtatt
     2821 ataaataata tttaggagaa aatattgtta aggaaatatc tggttatgtt tatttgttaa
     2881 ggtatatggg tttttataga aacccaggag cagctatggt aagaaaccca ggactcaaaa
     2941 aatgcctctc aacttctctc atatgttgcg gagtctggag tcccatttat accatagaaa
     3001 gcattggggg tatagcatgg taatttcaca tcggactcca taccgcggcc atctatataa
     3061 tattactgat ggccgcgcga agtcccggta tgccttttca gccgttggat ctggagccgt
     3121 acctttttcg cgttcccatt ttgcccctgg agtcccgagc tatttacgac cccctgccac
     3181 cgagtcccct tcgtggagcg ctcttcgtcc aacacgtgtc ccaacttttg cgcatttttg
     3241 actacctgtc gcgcacttga attaaagttg cccttttatt ttcttccaat tattttgcgg
     3301 ctgagtagtc tagtggatcc cccggagctc cacttgggat aggttaagaa aatatgtctg
     3361 gcttgtaaac gaaaagaatc ggggtttcta ggcatttttt aaatagtgac ccaggactca
     3421 aaaactagct ctcaacttct ctcatatgtt gtggagtctg gagtccttta tatatgttta
     3481 cgctatagaa ggcatttggg gactctcccg ggcccaccat aggactccat accgcggcca
     3541 tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc gttagcgcga
     3601 cctttaattc aaattaaagg ttgctttttt ctatcgtccg atcattttgc gcctgactag
     3661 cctagatatt agcaacaact tcgcggctaa gttgttgacc gtcaagtata aattaatggg
     3721 cttatggcct aatgggtata gagcaaaatg gcatgcctgc agactagtga gaccgtgtaa
     3781 gaggtctcga ttcgctagcg ttaactggcc ataatcctgt gtatgcaacg ttgaaaattc
     3841 ggatctattt ttacgattcg atcatgaatt aataaaattt aaattttatt gaatgatttt
     3901 ctagtacata actgacatac gacttatcag ttgcgaaacg aacagctcta attacattgt
     3961 taagtgaaat tacgcctaat tcgtctaagt acaacataac caactgttta aacctaatta
     4021 aataagtcga cccagaagct gtcagagatg tcgtccagac ttggaagttc aggtaagctt
     4081 tgtggagatg caacgctctc cgtaagttgt ggttgaatcg gatctggacg tggtatatac
     4141 gcgtcctggt gtatagcaga tcctctactc cgtatatctt gaaatatagg ggatttatta
     4201 tctcccagat atacacgcca ttctctgcct gacgtgcagt gatgatttcc cctgtgcgtg
     4261 aatccatgac cggtgcagcc tatgtggaag tatatggtgc acccacagtt tagatcaatg
     4321 cgtcgtcgtc taatcgccct cctcttggct tgcctgtgtg ccttcttgat agaggggggc
     4381 gtcgagagtg atgaagataa cattcttgag agtccaatgt ttgagcgacg cattttccgc
     4441 tttatccagg aaatctttat agctggatcc ctcgcctgga ttgcaaagca cgattgaagg
     4501 gatccctcct ttaatttgaa ctggtttccc gtacttgcaa tttgactgcc agtctttttg
     4561 ggccccaaga agctctttcc aatgctttag ctttaggtat tgcggtgcta cgtcatcaat
     4621 gacgttatag gcaacattat tcgagtacac tcgattattg aagtccaggt gtccactgag
     4681 gtaattatgt gggcctaaag cacgagccca catcgtcttg cccgttctag aatcaccttc
     4741 cactatgaga ctaactggtc ttacaggccg cgcagcggga cccattccaa aataatcatc
     4801 cgcccattct tgcatctgtt caggaacgtt agtgaaagag gaaagtggaa atggagggac
     4861 ccattgttca ggcgcctttt tgaaaaggcg ttccaagttc gctgtaatgt tatggtaact
     4921 cacgatgaac gcccttggat ctccggcccg gataattgca agagcttccg ccaaactagg
     4981 tgcattgatg gcgttataat agacgtcgtc tttattcttc tttgtacccc caggcacttt
     5041 gaactgtccg gattcacaac aatcgccgtc tttggtgatg taattcttga cggcgttggc
     5101 gtctttggct gcttgtatgt ttgggtgaaa attggtcgac cttctggggt gagtaaggtc
     5161 gaaaaatctc ttattcttga tgttggactt tccagagagt tggatgagac agtgaaggtg
     5221 tgggaatccg tccgagtgct cctctctggc aactcgaata tatgttggtt tgacgactga
     5281 ccatgataag gtttgaagca tttgaagagc ttcatttttt gggatatcgc acttgggata
     5341 ggttaagaaa atatgtctgg cttgtaaacg aaaagaatcg gggtttctag gcatttttta
     5401 aatagtgacc caggactcaa aaactagctc tcaacttctc tcatatgttg tggagtctgg
     5461 agtcctttat atatgtttac gctatagaag gcatttgggg actctcccgg gcccaccata
     5521 ggactccata ccgcggccat ctatataata ttactgatgg ccgcgcggat tttggagccc
     5581 ccttttgtcg ttagcgcgac ctttaattca aattaaaggt tgcttttttc tatcgtccaa
     5641 tcattttgcg cctgactagc ctagatatta gcaacaactt cgcgactaag ttgttgaccg
     5701 tcaagtataa attaatgggc ttatggccca atgggtatag agcaaaatgc ccaagcgtga
     5761 tgccccgtgg cgcttattag cgggtacgtc taaggtaagt cgaaatgcca actactctcc
     5821 cttgagagta ttgggcccta aagttaataa gggacctcga gggggggccc ggtacccggg
     5881 gatcctctag agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt
     5941 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     6001 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     6061 ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag
     6121 tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt
     6181 tattagaata atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta
     6241 tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc
     6301 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     6361 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     6421 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     6481 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     6541 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     6601 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     6661 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     6721 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     6781 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     6841 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     6901 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     6961 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     7021 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     7081 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     7141 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     7201 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     7261 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     7321 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     7381 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     7441 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     7501 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     7561 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     7621 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     7681 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     7741 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     7801 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     7861 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     7921 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     7981 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     8041 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     8101 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     8161 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     8221 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     8281 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     8341 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     8401 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     8461 cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     8521 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     8581 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     8641 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     8701 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     8761 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     8821 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     8881 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     8941 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     9001 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     9061 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     9121 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     9181 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     9241 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     9301 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     9361 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     9421 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     9481 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     9541 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     9601 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tgtctttcct
     9661 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     9721 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     9781 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     9841 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     9901 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     9961 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
    10021 tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga
    10081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
    10141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
    10201 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
    10261 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
    10321 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    10381 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    10441 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    10501 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    10561 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    10621 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    10681 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    10741 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    10801 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    10861 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    10921 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
    10981 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    11041 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    11101 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    11161 aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa
    11221 atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg
    11281 acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac
    11341 cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc
    11401 acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt
    11461 tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag
    11521 ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg
    11581 ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg
    11641 cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag
    11701 caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag
    11761 tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt
    11821 tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca
    11881 ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag
    11941 cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat
    12001 ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga
    12061 actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa
    12121 agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt
    12181 gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca
    12241 tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag
    12301 caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc
    12361 tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg
    12421 caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac
    12481 gtttttaatg tactgaatta acgccgaatt aattcgggga tagccctttg gtcttctgag
    12541 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc
    12601 tgctctagcc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
    12661 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    12721 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    12781 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acg
//
