LOCUS       sequence-476000-        2120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' Operon Connector 4 in the M1 position used to construct operons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_0_1-2_004_OC5'4
FEATURES             Location/Qualifiers
     source          1..2120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      64..121
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(145..162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(316..904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(396..415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1087..1181)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1202..1861)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1776..1795
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2023..2041
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2045..2088)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2091..2112
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaac acgtctcgca agtacttgag accactcatc gcgactagta gcggccgctg
       61 cagtccggca aaaaagggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
      121 attacttcgc gttatgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      181 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      241 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      301 cgcgttgctg gcgtttttcc acaggctccg cccccctgac gagcatcaca aaaatcgacg
      361 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      421 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      481 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      541 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      601 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      661 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      721 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      781 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      841 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      901 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      961 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1021 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagctcgag
     1081 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1141 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1201 attacgcccc gccctgccac tcgtcgcagt actgttgtaa ttcattaagc attctgccga
     1261 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1321 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1381 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgatacg aaaaacatat
     1441 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1501 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagt gatgaaaacg
     1561 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1621 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1681 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1741 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     1801 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     1861 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     1921 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaactcg agtgccacct
     1981 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2041 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
     2101 ccgcttctag agcgatgagt
//
