LOCUS       sequence-47602-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NpHR3.0-eYFP in cells lacking Cre (Cre-Off), for 
            optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DO-NpHR3.0-eYFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(37..70)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(121..154)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcgtgaggta ccggatcctc tagagtcgac tccggaataa cttcgtatag gatactttat
       61 acgaagttat gcagaatggt agctggattg tagctgctat tagcaatatg aaacctctta
      121 ataacttcgt atagcataca ttatacgaag ttatggcgcg ccttatctag atttagccac
      181 catgacagag accctgcctc ccgtgaccga gagtgccgtg gcccttcaag ccgaggttac
      241 ccaaagggag ttgttcgagt tcgtgctgaa cgaccctttg cttgcaagca gtctctatat
      301 caacatcgca cttgcaggac tgagtatact gctgttcgtt tttatgaccc gaggactcga
      361 tgatccacgg gcaaaactta ttgctgtgtc aaccatcctt gtgcctgtcg tcagcattgc
      421 ctcctacact ggattggcga gcggcctgac aatttccgtt cttgaaatgc cagcgggcca
      481 ttttgcagaa ggcagctcag tgatgctggg aggagaagag gtagatggtg tagtcaccat
      541 gtggggacgg tatctcacct gggcactttc cacgcccatg attctcctcg ctctgggtct
      601 cctggccgga agcaatgcta caaagctctt cacagctatc acgttcgata tcgctatgtg
      661 cgtgactggc cttgccgcgg ccctgactac ctcctcccac ctcatgagat ggttctggta
      721 cgctatcagt tgtgcatgct ttctggtggt cttgtatatc ctgctggtgg agtgggcaca
      781 ggacgccaaa gccgcgggaa ccgctgacat gttcaatacc ctgaagctgt tgacagtagt
      841 gatgtggctg gggtatccaa
//
