LOCUS       sequence-476040-       12346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: mEos2-Talin-N-22
FEATURES             Location/Qualifiers
     source          1..12346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      577..586
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8272..8952
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    9074..9195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9111..9130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9165..9184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(9202..9657)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9339..9360)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9551..9570
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9684..9788
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9790..10147
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9790..9981
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9813..9833)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9998..10133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10182..10976
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             10182..10976
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10236..10255)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10846..10865
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11164..11183)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11208..11255
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11584..12172
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12073..12092
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attctagcca ccatggttgc gctttcgctg
      601 aagattagca ttgggaatgt ggtgaagacg atgcaatttg agccatctac catggtgtac
      661 gacgcctgcc gcatgattcg tgagcggatc ccagaggccc tggctggccc tcccaacgac
      721 tttgggctct ttctgtcaga tgatgacccc aaaaaaggca tctggctaga ggctgggaaa
      781 gctttggact actacatgct ccgaaatggg gacaccatgg agtacagaaa aaagcagaga
      841 cccctgaaga tacggatgtt agacggaacg gtgaagacta tcatggtgga tgactccaag
      901 accgtcacag acatgctcat gaccatttgt gcccgaattg gtatcaccaa ccatgatgag
      961 tattcactgg ttcgagagct gatggaagaa aagaaagatg aggggacagg gaccctgaga
     1021 aaagacaaga ccctactgcg agatgaaaag aagatggaga agctaaagca gaagttgcac
     1081 acagacgatg agttgaactg gctggaccat gggcggacac tgagggaaca gggagtggaa
     1141 gagcatgaga cgttgctgct gcggagaaag ttcttctact cagaccagaa tgtggattcc
     1201 cgagaccctg tacagctgaa ccttctctat gtgcaggcac gagatgacat cctgaatggc
     1261 tcccatcctg tctcctttga caaggcctgt gaatttgcag gcttccagtg ccagatccag
     1321 tttggacctc acaatgaaca gaagcacaag gctggcttcc ttgacctgaa ggacttcctg
     1381 cccaaggagt atgtgaagca gaagggagag cgtaagatct ttcaggcaca caagaattgt
     1441 gggcagatga gtgaaattga ggccaaggta cgctacgtga agctggcccg ttccctcaag
     1501 acttacggtg tctccttctt cctagtcaag gaaaagatga aggggaagaa taaactggtg
     1561 cccaggctgt tgggcatcac taaggagtgt gtgatgcgtg tggatgagaa gaccaaggag
     1621 gtgattcagg aatggagcct caccaacatc aaacgctggg ctgcctctcc caagagcttt
     1681 actctggact ttggagacta tcaggatggc tactactcag tacagacgac agaaggcgag
     1741 cagatcgcac agctcattgc tggctacata gatatcatcc ttaagaagaa aaaaagcaag
     1801 gaccattttg ggctggaggg agatgaagag tctactatgt tggaggactc agtgtctccc
     1861 aaaaagtcaa cagtccttca gcagcagtac aaccgagtgg ggaaagtgga gcacggctct
     1921 gtggctctgc cagccatcat gcgctctgga gcctctggtc ctgagaattt ccaggtgggg
     1981 agcatgccac ctgcccagca gcagatcacc agtggccaga tgcaccgagg acacatgcca
     2041 cctctgactt cagcccagca ggcgctcact ggaaccatta actccagcat gcaggctgtg
     2101 caggctgccc aggccactct ggatgacttt gagactctac cccctcttgg ccaggatgct
     2161 gcctccaaag cttggcgtaa gaacaagatg gatgaatcaa agcatgagat ccactcccag
     2221 gtagatgcca tcacagctgg cactgcctct gtggtaaacc tgacagcagg agatcctgca
     2281 gagacagact atacagcagt gggctgtgca gtcaccacca tctcttccaa cctgacggag
     2341 atgtcccgtg gggtgaagct attggctgcc ctgctggagg atgaaggcgg caatggccgg
     2401 ccccttctgc aagcagcaaa gggccttgca ggggctgtgt cggaactgct tcgcagtgct
     2461 cagcccgcca gcgctgagcc tcgtcagaac ctgctgcagg cagccgggaa cgtgggccag
     2521 gccagtgggg agctgttgca gcaaattggg gaaagtgaca ctgaccccca cttccaggat
     2581 gttctaatgc agctagcaaa cgcagtggca agtgctgcag ccgccctggt cctcaaggcc
     2641 aagagtgtgg cccagcgcac agaggattcc gggcttcaga cccaagtgat tgctgcagct
     2701 acgcaatgtg ctctgtccac ttcccagctg gtggcctgta ccaaggtggt agcacctaca
     2761 atcagctcac ctgtgtgcca agaacagcta gtcgaggctg ggcgactggt ggccaaagct
     2821 gtggagggct gtgtgtctgc ctcgcaagca gctacagaag atggacagct gcttcgaggg
     2881 gtaggagcag cagccacggc tgtcacccag gccctcaatg agctgctgca gcacgtgaag
     2941 gcccacgcca ccggagctgg gcctgccggt cggtatgacc aagccactga taccatcctc
     3001 accgtcactg aaaacatctt cagctccatg ggtgatgctg gggagatggt gcgccaggcc
     3061 cgcatcctgg cccaagccac ctcagacctg gtcaatgcta tcaaggccga tgctgagggg
     3121 gagagtgatc tggagaactc tagaaagctc ctgagtgctg ccaagatcct cgctgatgcc
     3181 accgccaaga tggtggaggc ggccaaggga gcagccgccc accctgacag tgaggaacag
     3241 cagcagcgac tgcgtgaagc agctgagggg cttcgcatgg ccaccaatgc agctgcgcag
     3301 aacgccatca agaagaagtt ggtgcagcgc ctggagcatg cagccaagca agctgcagcc
     3361 tctgcaacac agaccattgc tgcagcccaa catgcagcct ctgcccccaa ggcctctgcg
     3421 ggcccccagc ccctactggt gcagagctgt aaggccgtgg cagagcagat tccgttgctg
     3481 gtgcagggtg tccgaggaag tcaagctcag cctgacagcc ctagtgctca gctcgccctc
     3541 attgctgcca gccagagctt cctgcagcca ggtgggaaga tggtggcagc ggcaaaagcc
     3601 tcagtgccaa caattcagga ccaggcttca gcgatgcagc tgagtcagtg cgcaaagaac
     3661 ctaggcactg ccctggccga gctgcgcact gctgctcaga aggctcagga ggcatgtgga
     3721 cctttggaga tggattctgc actgagtgtt gtacaaaatc tagagaaaga cctacaggaa
     3781 ataaaagcag cagctcgaga tggcaagctt aagcccttac ctggggaaac aatggagaag
     3841 tgtacccaag atcttggcaa cagcaccaaa gcggtgagct ctgccatcgc caagctgctg
     3901 ggggagattg cccagggcaa cgagaactat gcaggtattg cagctcggga tgtagctggt
     3961 ggactaaggt cactagccca ggctgcgcgg ggtgtggctg cgctgacatc agatcctgca
     4021 gtgcaggcca ttgtgcttga cacagccagc gacgttctgg acaaggccag cagcctcatt
     4081 gaagaggcaa aaaaagcatc tggacaccca ggggacccag aaagccagca aaggcttgct
     4141 caggtagcta aagcagtgac ccaagccctg aaccgctgtg tcagctgcct gcctggccaa
     4201 agagacgtgg ataatgccct acgagccgtt ggagatgcca gcaagcgact cctgagtgac
     4261 ttgcttcctc caagcacggg gacatttcaa gaagcacaga gccgattgaa tgaagctgca
     4321 gctgggttaa atcaggccgc cacagaactg gtgcaagcct cccgaggaac tcctcaggac
     4381 ctggctcggg cctccggccg atttggacag gacttcagca ccttcctgga agctggtgtg
     4441 gagatggccg gacaggcccc gagccaggag gaccgagccc aggtggtgtc caacctgaag
     4501 ggcatatcca tgtcttcaag caaacttctc cttgccgcta aggccttgtc cacagaccct
     4561 gcttctccca acctcaagag tcagctggct gcggctgccc gggcagtgac ggacagcatc
     4621 aaccagctca tcaccatgtg cacccagcag gcacctggcc agaaggagtg tgacaatgca
     4681 cttcggcagc tagagacagt ccgagaactc ctggagaatc cagtccagcc catcaacgac
     4741 atgtcctact tcggttgctt ggacagtgtc atggagaact ccaaggtcct aggtgaggcc
     4801 atgactggca tctcccaaaa tgccaagaat ggaaatctgc cggagtttgg agatgccatt
     4861 gccacagcct ccaaggctct ctgtggcttc acggaggcag ccgcacaggc agcatatcta
     4921 gttggtgtct ctgaccccaa cagccaagct ggacagcaag gactggtgga acccacacag
     4981 tttgcccgtg caaaccaggc aattcagatg gcctgtcaga gtctggggga gcctggctgt
     5041 acccaggccc aggtgttatc tgcagccact attgtagcca aacacacatc tgcattgtgt
     5101 aacagctgtc gcctggcttc cgctaggact gccaatccca ctgccaagcg ccagtttgta
     5161 cagtcagcca aggaggtggc caacagtaca gccaatcttg tcaagaccat caaggcacta
     5221 gatggggact tcacagaaga gaaccgtgcc cagtgccgag cagccacagc ccctctgctg
     5281 gaagctgtgg ataacctgag tgcctttgcc tccaaccctg agttctccag cgtccctgcc
     5341 cagatcagcc ctgagggcag agcagccatg gagcccattg taatctctgc taagacaatg
     5401 ttggagagtg ctggagggct catccagaca gctcgggcgt tagcagtcaa tccccgagac
     5461 cccccacgtt ggtctgtgct agctggccac tctcggactg tctcagattc catcaagaaa
     5521 cttattacaa gcatgaggga caaagcccca gggcagctgg agtgtgagac agccattgcg
     5581 gctctgaaca gctgcttgcg ggacctagat caggcttcgc ttgccgctgt cagccagcag
     5641 cttgctcccc gtgaaggaat ctctcaagag gctttgcaca cccagatgct cactgcagtg
     5701 caagaaattt ctcatctcat tgagccgctg gccagcgctg ctcgggctga agcctcccag
     5761 ctgggacaca aggtgtccca aatggcccag tactttgaac cactcaccct ggctgcagtg
     5821 ggtgctgcgt ctaagaccct gagccacccg cagcagatgg cacttctgga ccagactaaa
     5881 acgttggcag agtctgcctt gcagttgcta tatactgcca aggaggctgg tggtaacccc
     5941 aagcaagcag cacacaccca ggaagccctg gaggaggctg tgcagatgat gacagaggcc
     6001 gtagaagacc tgacgacgac cctcaacgaa gcagccagtg ctgcaggagt cgttggcggc
     6061 atggtggact ctatcactca ggccatcaac cagctagatg aaggacctat gggtgaccca
     6121 gaaggctcat tcgtagatta ccagacaacc atggtgagga cagccaaggc cattgctgtc
     6181 actgttcagg agatggtaac caagtcaaac accagccctg aagagctagg ccctcttgcc
     6241 aaccagctga ccagtgacta tggccgactg gcctcacaag ccaagcctgc agctgtggct
     6301 gctgaaaatg aagagatagg cgctcacatc aaacaccgag tacaggagct gggccatggc
     6361 tgctctgctc tggtcaccaa ggcaggtgcc ttgcagtgta gccccagtga tgtctacacc
     6421 aagaaggagc tcatagagtg tgcccgcaga gtgtcagaga aggtctccca tgttctggct
     6481 gcactccagg ctgggaatcg tggtacccag gcctgcatta cagcagccag tgctgtgtct
     6541 ggtatcattg ctgacctcga caccaccatc atgttcgcta ctgctggcac acttaaccgt
     6601 gagggtgctg aaacttttgc tgaccaccgg gagggtatct taaagacagc aaaggttctt
     6661 gtggaggaca ccaaggtcct agtgcagaat gcagctggga gccaggagaa gttggcacaa
     6721 gccgcccagt cctccgtggc caccattacc cgcctcgctg atgtggtcaa gctcggtgca
     6781 gccagcctag gagccgaaga ccctgaaact caggtggtgc tgatcaatgc agtaaaggac
     6841 gtagccaagg ccctgggtga cctcatcagc gctacgaagg ctgcagcggg caaagttggg
     6901 gatgaccctg cagtgtggca gctcaagaac tctgccaagg tgatggtgac caatgtgaca
     6961 tcattgctca agacagtgaa ggctgtggaa gatgaggcca ccaaaggcac acgggcccta
     7021 gaggcaacca cagagcacat acgtcaggaa ctggcggtct tctgttcccc agagccacct
     7081 gccaagacct ctacccctga agacttcatc cgaatgacca agggtattac tatggcaaca
     7141 gccaaagccg ttgctgctgg caattcctgt cgacaggaag atgtcattgc cacagccaat
     7201 ctgagccgac gggctattgc ggacatgctt cgggcttgca aggaagcagc tttccaccca
     7261 gaagtggcgc ctgatgtacg gctccgagcc ctgcactatg gccgggagtg tgccaatggt
     7321 tacctggaac tgctggacca cgtgctgctg acccttcaga agccaaaccc agacctgaag
     7381 cagcagctaa ctggacactc aaagcgggtt gctggctctg tgactgagct catccaggct
     7441 gctgaagcca tgaaaggaac agagtgggtg gacccagagg accctactgt cattgctgag
     7501 aatgagctct tgggagccgc agccgccatc gaggctgcag ccaagaagct ggagcagctg
     7561 aagcccaggg ccaaacccaa ggaggcggat gagtccttga actttgagga acaaatccta
     7621 gaagctgcca agtccatcgc tgcagccacc agtgcactgg taaaggctgc gtcagcagcc
     7681 cagagggagc tggtggctca aggaaaggtg ggcgccattc cagccaatgc actggatgat
     7741 gggcagtggt cgcagggcct catttccgct gcccgtatgg tggctgcggc caccaacaat
     7801 ctgtgtgaag cagctaacgc agctgtccag ggccatgcta gccaggagaa actcatttcc
     7861 tcagccaagc aagtagctgc ctccacagct cagctcctgg tagcttgcaa ggtcaaagca
     7921 gatcaggact ctgaggcaat gaaacggctc caggctgctg gcaatgcagt gaagagggcc
     7981 tcagataacc tggtaaaggc ggcccagaaa gctgcagcct tcgaagacca ggagaatgag
     8041 acggtggtgg tgaaggagaa aatggttggg ggcattgccc agattatcgc agcacaggaa
     8101 gagatgcttc ggaaggaacg agagctggaa gaggctcgga aaaagctcgc ccagatccgg
     8161 cagcagcagt acaagttctt gccttcagag cttcgagacg agcacggcgc tgggtccggg
     8221 tccgggcaag ggtccggcgc aggctccgcg tcccgatcac cggtcgccac catgagtgcg
     8281 attaagccag acatgaagat caaactccgt atggaaggca acgtaaacgg gcaccacttt
     8341 gtgatcgacg gagatggtac aggcaagcct tttgagggaa aacagagtat ggatcttgaa
     8401 gtcaaagagg gcggacctct gccttttgcc tttgatatcc tgaccactgc attccattac
     8461 ggcaacaggg tattcgccaa atatccagac aacatacaag actattttaa gcagtcgttt
     8521 cctaaggggt attcgtggga acgaagcttg actttcgaag acgggggcat ttgcattgcc
     8581 agaaacgaca taacaatgga aggggacact ttctataata aagttcgatt ttatggtacc
     8641 aactttcccg ccaatggtcc agttatgcag aagaagacgc tgaaatggga gccctccact
     8701 gagaaaatgt atgtgcgtga tggagtgctg acgggtgata ttcatatggc tttgttgctt
     8761 gaaggaaatg cccattaccg atgtgacttc agaactactt acaaagctaa ggagaagggt
     8821 gtcaagttac caggctacca ctttgtggac cactgcattg agattttaag ccatgacaaa
     8881 gattacaaca aggttaagct gtatgagcat gctgttgctc attctggatt gcctgacaat
     8941 gccagacgat aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     9001 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     9061 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     9121 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     9181 catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9241 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9301 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9361 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9421 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9481 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9541 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9601 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag
     9661 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9721 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9781 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
     9841 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     9901 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     9961 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    10021 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    10081 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    10141 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat
    10201 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    10261 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
    10321 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
    10381 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    10441 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    10501 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    10561 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    10621 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    10681 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
    10741 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    10801 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    10861 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    10921 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    10981 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
    11041 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
    11101 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct
    11161 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
    11221 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg
    11281 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc
    11341 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg
    11401 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact
    11461 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    11521 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    11581 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    11641 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    11701 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    11761 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    11821 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    11881 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    11941 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    12001 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    12061 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    12121 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    12181 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
    12241 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt
    12301 aatcaattac ggggtcatta gttcatagcc catatatgga gttccg
//
