LOCUS       sequence-476041-        6741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HshnRNPC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6741)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3.1-MS2-HA-HshnRNPC1_Z
FEATURES             Location/Qualifiers
     source          1..6741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             164..190
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(1125..1143)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1126..1143)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1163..1180)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1169..1393
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1439..1867
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1526..1545)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1736..1757
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1876..1896)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1881..2210
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2061..2196
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2123..2142
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2277..3071
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2331..2350)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2941..2960
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3245..3366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3282..3301)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3336..3355
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3415..3431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3415..3431)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3428..3450)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3439..3455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3463..3469,3470..3487,3488..3493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3508..3529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3646..3663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3817..4405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3897..3916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4576..5367,5368..5436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5199..5218
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5437..5541)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5616..5635)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        5807..6186
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6187..6390
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6341..6361
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6435..6454
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6435..6453
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      6467..6476
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actaaccatt ccaattttcg ccacgaattc cgactgcgag cttattgtta aggcaatgca
       61 aggtctccta aaagatggaa acccgattcc ctcggccatc gcggccaact ccggcatcta
      121 cggaggtgga ggtggatctg gtggatctcc tccacatatg gcttacccat acgatgttcc
      181 agattacgct cctccaaagc ttggatccat ggccagcaac gttaccaaca agacagatcc
      241 tcgctccatg aactcccgtg tattcattgg gaatctcaac actcttgtgg tcaagaaatc
      301 tgatgtggag gcaatctttt cgaagtatgg caaaattgtg ggctgctctg ttcataaggg
      361 ctttgccttc gttcagtatg ttaatgagag aaatgcccgg gctgctgtag caggagagga
      421 tggcagaatg attgctggcc aggttttaga tattaacctg gctgcagagc caaaagtgaa
      481 ccgaggaaaa gcaggtgtga aacgatctgc agcggagatg tacggctcct cttttgactt
      541 ggactatgac tttcaacggg actattatga taggatgtac agttacccag cacgtgtacc
      601 tcctcctcct cctattgctc gggctgtagt gccctcgaaa cgtcagcgtg tatcaggaaa
      661 cacttcacga aggggcaaaa gtggcttcaa ttctaagagt ggacagcggg gatcttccaa
      721 gtctggaaag ttgaaaggag atgaccttca ggccattaag aaggagctga cccagataaa
      781 acaaaaagtg gattctctcc tggaaaacct ggaaaaaatt gaaaaggaac agagcaaaca
      841 agcagtagag atgaagaatg ataagtcaga agaggagcag agcagcagct ccgtgaagaa
      901 agatgagact aatgtgaaga tggagtctga ggggggtgca gatgactctg ctgaggaggg
      961 ggacctactg gatgatgatg ataatgaaga tcggggggat gaccagctgg agttgatcaa
     1021 ggatgatgaa aaagaggctg aggaaggaga ggatgacaga gacagcgcca atggcgagga
     1081 tgactcttaa ctcgagtcta gagcggccgc ctagagggcc ctattctata gtgtcaccta
     1141 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     1201 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     1261 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     1321 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     1381 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     1441 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     1501 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     1561 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     1621 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     1681 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     1741 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     1801 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1861 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     1921 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     1981 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     2041 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2101 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     2161 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     2221 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     2281 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2341 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     2401 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     2461 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     2521 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     2581 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     2641 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     2701 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     2761 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     2821 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     2881 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     2941 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3001 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3061 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3121 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3181 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3241 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     3301 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     3361 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     3421 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     3481 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     3541 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     3601 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     3661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     4921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     5341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     5401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     5461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     5521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     5581 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     5641 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     5701 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     5761 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     5821 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     5881 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     5941 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6001 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6061 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6121 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6181 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     6241 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     6301 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     6361 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     6421 tggcttatcg aaattaatac gactcactat agggagaccc aagcttgcca ccatggcttc
     6481 taactttact cagttcgttc tcgtcgacaa tggcggaact ggcgacgtga ctgtcgcccc
     6541 aagcaacttc gctaacgggg tcgctgaatg gatcagctct aactcgcgat cacaggctta
     6601 caaagtaacc tgtagcgttc gtcagagctc tgcgcagaat cgcaaataca ccatcaaagt
     6661 cgaggtgcct aaagtggcaa cccagactgt tggtggtgaa gagcttcctg tagccggatg
     6721 gagatcttac ttaaatatgg a
//
