LOCUS       sequence-47605-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by 
            alternative lox FAS sites), for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(123..833,834..836))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(165..186)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     514..533
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     773..794
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tctttacaat tttgtaatcc agaggttgat tatcgataag cttgatatcg aattcacaac
       61 ttcgtataga aaggtatata cgaagttgtg ctagctcatt acacctcgtt ctcgtagcag
      121 aacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      181 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactggta gctcaggtag
      241 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      301 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
      361 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      421 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      481 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      541 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      601 ttcatgtggt cggggtagcg ggcgaagcac tgcaggccgt agccgaaggt ggtcacgagg
      661 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      721 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      781 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccacg
      841 ttgatgtcga tctggtccag ggggatgtac tcgccctcgc tggtgatcct gctcttggcg
//
