LOCUS       sequence-476065-        2184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psbD 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3_031_psbD_5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      128..185
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(209..226)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(380..968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(460..479)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1151..1245)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1266..1925)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1840..1859
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2087..2105
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2109..2152)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2155..2176
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac tacacaatga ttaaaattaa ataataataa atttaacgta acgatgagtt
       61 gtttttttat tttggagata cacgcaaatg tgagaccact catcgcgact agtagcggcc
      121 gctgcagtcc ggcaaaaaag ggcaaggtgt caccaccctg ccctttttct ttaaaaccga
      181 aaagattact tcgcgttatg caggcttcct cgctcactga ctcgctgcgc tcggtcgttc
      241 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      301 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      361 aggccgcgtt gctggcgttt ttccacaggc tccgcccccc tgacgagcat cacaaaaatc
      421 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      481 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      541 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      601 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      661 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      721 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      781 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      841 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      901 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      961 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1021 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1081 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagct
     1141 cgaggcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1201 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1261 tcaaattacg ccccgccctg ccactcgtcg cagtactgtt gtaattcatt aagcattctg
     1321 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1381 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1441 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga tacgaaaaac
     1501 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1561 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagtgatgaa
     1621 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1681 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1741 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1801 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1861 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1921 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1981 gatcttattt cattatggtg aaagttggaa cctcttacgt gcccgatcaa ctcgagtgcc
     2041 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2101 gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt tctggaattc
     2161 gcggccgctt ctagagcgat gagt
//
