LOCUS       sequence-476071-        2912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  petG-rps3 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_004_petG-rps3_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      856..913
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(937..954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1108..1696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1188..1207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1879..1973)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1994..2653)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2568..2587
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2815..2833
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2837..2880)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2883..2904
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac ttatttataa actaaactac ctccaatgaa agaggtagtt tataacccaa
       61 tcaaaatgtt ttaccttttc ttcttctatg gacggaatac aaataacaat tgcattgtta
      121 ctctcgactg gtttctagct ttactgtcag gtgtttatat tatgctactg taccttttcc
      181 caagcaagaa tatacttaag gtatagaaat ataaacaagc atcccgaaga aaaaaggggt
      241 tattttctat gtttattaca ctaaagacat ttgatttggt ttaatctata taattgaaaa
      301 tagctttatt cttttattaa cgtatgggaa ccttttactg tttagtttgt ttttgcctat
      361 ttgtaaaggt ctatttaaat atatcctttt tgctttgccg caactaaaaa gtttacttag
      421 atactttcgt agctccattt acttgccggg gatttgttag ctcttttcaa agaaatgatt
      481 tttcacgttg gaaaaatcaa ctttgtattt agtactctac taacaaatac tatttaattc
      541 ctagtaatgg agtagtattg gatggttaat tcttatttta aacagagtaa gcattactta
      601 cttgtaattt taattataat taaatttatt tataaacagg ttctaaagaa gctataacac
      661 acattgtatt ttattttttt taacgttgya caaatgttta gaataaaaat acgtgttttg
      721 caccttgaaa tggctccagt tttctaaact aatgtttcaa aggagatgtg ttcaacatca
      781 attagcttac tttaatttca tttaaaaaat ttgtaatgtg agaccactca tcgcgactag
      841 tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt
      901 aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc
      961 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     1021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     1081 ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca
     1141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     1201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     1261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     1561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1681 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1741 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1801 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1861 tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1921 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     1981 gctcgatatc aaattacgcc ccgccctgcc actcgtcgca gtactgttgt aattcattaa
     2041 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2101 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     2161 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgata
     2221 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2281 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     2341 gtgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2401 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     2461 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2521 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2581 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2641 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2701 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaact
     2761 cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     2821 cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     2881 tggaattcgc ggccgcttct agagcgatga gt
//
