LOCUS       sequence-476073-        8135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  21X FLAG tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8135)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 8135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-20XFLAG
FEATURES             Location/Qualifiers
     source          1..8135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1322..1355)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          2363..2428
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2558..2578
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2850..2984
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2887..2906)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(2985..3018)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             4290..4313
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4326..4349
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4362..4385
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4398..4421
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4434..4457
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4488..4511
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4524..4547
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4560..4583
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4596..4619
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4632..4655
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4686..4709
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4722..4745
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4758..4781
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4794..4817
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4830..4853
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4884..4907
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4920..4943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4956..4979
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4992..5015
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5028..5051
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5064..5087
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(5959..5975)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5959..5975)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5972..5994)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5983..5999
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6007..6013,6014..6031,6032..6037))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6052..6073
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6190..6207)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6361..6949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6441..6460)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7120..7935)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7120..7935)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7843..7862
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(7936..8040)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8108..8126
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctaaggtcta ttttaaatgt
      421 gcatcagagc tactagttca ttagtgcata tttgaatatt agaatactac ctaaaataca
      481 tttttaatgc tattatgtta tacattctgt tgtttgatct ttaattttac aaactacatt
      541 ataaataact atattatact ttttatgata attactttta actaaacaat tcattattct
      601 gcttaaaatt caagggtatt cccactctcg atcttcgaat tcttctattt tattttcatt
      661 ttcgttgggg cgccccgtgt ctgtgttaat tgccccgggt aattgttgct gcctctcgag
      721 cattgaatat gcacttcccc ggcagtgccc cctccatttt ggccacgatc cggcatccac
      781 atccctatcc cgcatcccca tctccaacca aaagcaacag ccccatctcc atgtttgtgt
      841 aatccgcgcg ctcggacgcg tttccctgcc gcctgttggc ttaaacagga ttttcgaaat
      901 ggaaaacttc tgtagcaagt gtttggccat ctgtccctgt tctcgactcg cctgccgctg
      961 cacttgtgcg gatctgcaac ctaatttgca atgcccgagc atgtgagaca gttaatcaca
     1021 cagaatcact ttgaaaacta tgaaccgtgc cagcaacaac aggtctggcc aactaaaaat
     1081 attttgccag cactttatga tctctaaatc aatatcagtg tcggaggacc ggaagcaata
     1141 gaacagatat tcttaagtct taagttctta agtagcttgt acagctcttt aaatttgtaa
     1201 aataatataa cttataatat tagttaattg gaaaatgata caatcagtac ataattagct
     1261 gaaacgattt taggatgttt atgttaatca agacagttta ggatctttgt aatatctaag
     1321 agaagttcct atactttcta gagaatagga acttcgcgtc gcgccacttc aacgctcgat
     1381 gggagcgtca ttggtgggcg gggtaaccgt cgaaatcagt gtttacgctt ccaatcgcaa
     1441 caaaaaattc actgcaacac tgaaaagcat acgaaaacga tgaagattgt acgagaaacc
     1501 ataaagtatt ttatccacaa agacacgtat agcagaaaag ccaagttaac tcggcgataa
     1561 gttgtgtaca caagaataaa atcggccaga ttcagtgttg tcagaaataa gaaaacccca
     1621 ctatgttttt ctttgccttt tctttctccc agcgatcatt catttcgtgg tgaaagaacg
     1681 gggtcattgc acggagtttc gactgcggga aagcagagct gccgttcact tcgtctataa
     1741 ttagcgcttt ctattttccc cgattcgggc cgctgctgcg cttttccgcc tgctgtttgt
     1801 ggcaagtgta gcagcaggct gtgcacgcag tgtggcatgc acttggcttt ccaccgttgg
     1861 tatcgattct ctgggacgat gagtcattcc tttcgggacc acagcataat cgttgccagc
     1921 tcaccgaaat ggtgacttca tttcttaact gccgtcaagc atgcgattgt acatacatac
     1981 atatttatat atgtacatat ttatgtgact atggtaggtc gatataatag caatcaacgc
     2041 aagcaaatgt gtcagtcctg cttacaggaa cgattctatt tagtaatttt cgttgtataa
     2101 agtaattatg tatgtatgta agccccataa atctgaaaac cggtactagt acgaaagtcg
     2161 atccaacatg gcgacttgtc ccatccccgg catgtttaaa tatactaatt attcttgaac
     2221 taattttaat caaccgattt atctctcttc cgcaggtcta actaactaac aaaattgact
     2281 aagtgagatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     2341 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     2401 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     2461 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     2521 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     2581 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     2641 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     2701 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     2761 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaactcga
     2821 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2881 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2941 gtggtttgcc caaactcatc aatgtatctt atcatgtctg gatcgaagtt cctatacttt
     3001 ctagagaata ggaacttcgt attttattac taaagatttt ccataaaatc tatgtgccca
     3061 aagtattcta ttgcacctcg tgacctttta ccgcattaat gttgcttttt cattttcagg
     3121 taacgagctt caccaccgcc aagaaaggaa caataccgat gccctcctcg agcggcgtgt
     3181 ccgctttgca gctgcaccag cagcgccaaa tagccaccat cgagacacca ccgaacagcg
     3241 ccagcctacc gatgcagata cccacggtga ccatggacct ggcctccgac atatccacct
     3301 cggaggcggg ggaactggag gcggtggccg cccaaacggt gcttgcctat gcaaatccca
     3361 atggtgcggg cacgaattcg gtcaccgtgg tgagcagcgg gaacgggggc cctggggcgt
     3421 cgggtgttcc acccagctcg ccgaaggaga cgcttcaggt gagcacgata gccaccggtc
     3481 gggtgggtct caatgtgccc gtgccaccgt ccatgggcag caatagttcc atcaatgtgc
     3541 tgaacaacaa caacggtacc gtggcgatgg atggaggtgc caccgccgcc ggcgggggaa
     3601 atggtagtgc tgtagcgacg cctggtggac tgcagcaaac gggcggtaat gagctgcgcg
     3661 tctctccgat cgccggacgg tgtcgagccc tatccgtggg cgtggacacg gacatggtga
     3721 gtgaattcga tccatccagc agcgattcgg gcgtggtcag tcgctgtgcc gtggtcaagc
     3781 cgctcaagtt tcgcctttgc cagccgatct tcgggcgcaa gtcgagtaac cagcagcggc
     3841 gcaacgaggc caaagcggcg gccaagaatc aacagtccag tggagtggcc agcaaaaatc
     3901 agcagcagca gcagcagcaa cagcagcagc aggtagtaaa gtcggaactg gagccggcca
     3961 taccgaagac accgaagccc agggatccgg agaaggagaa gcgacgaatt gcgcgaaaga
     4021 aggagaagcg ggccacccta attctgggcc tgatcatggg cagcttcatc gcctgctggc
     4081 tgcccttctt cttcctgtac atcctggtgc cggcatgtag cagccattgc aacataccgg
     4141 aatcggcgtt cgccgtcgcc ttctggctgg gctacatgaa ttcggcgctc aatccggcca
     4201 tctataccat cttcaacaag gacttccgac gcgccttccg gcgcatcctg ttcaagcggc
     4261 gcgcccttaa gctcgagggc tccggcggcg attacaagga tgacgacgat aagggctccg
     4321 gcggcgatta caaggatgac gacgataagg gctccggcgg cgattacaag gatgacgacg
     4381 ataagggctc cggcggcgat tacaaggatg acgacgataa gggctccggc ggcgattaca
     4441 aggatgacga cgataagggc tccggcggcg tcgagggctc cggcggcgat tacaaggatg
     4501 acgacgataa gggctccggc ggcgattaca aggatgacga cgataagggc tccggcggcg
     4561 attacaagga tgacgacgat aagggctccg gcggcgatta caaggatgac gacgataagg
     4621 gctccggcgg cgattacaag gatgacgacg ataagggctc cggcggcgtc gagggctccg
     4681 gcggcgatta caaggatgac gacgataagg gctccggcgg cgattacaag gatgacgacg
     4741 ataagggctc cggcggcgat tacaaggatg acgacgataa gggctccggc ggcgattaca
     4801 aggatgacga cgataagggc tccggcggcg attacaagga tgacgacgat aagggctccg
     4861 gcggcgtcga gggctccggc ggcgattaca aggatgacga cgataagggc tccggcggcg
     4921 attacaagga tgacgacgat aagggctccg gcggcgatta caaggatgac gacgataagg
     4981 gctccggcgg cgattacaag gatgacgacg ataagggctc cggcggcgat tacaaggatg
     5041 acgacgataa gggctccggc ggcgattaca aggatgacga cgataagggc tccggcggcg
     5101 tcgacggatc cgcggccgca tgatgtttgg cgtatgtgtg ctgttacagg tgcgtttacg
     5161 ccagcatcga gaaggcaacc gaacgtgcca tgggcagcac accaatgggc tatggtcatg
     5221 gcatgtatca gcactacacg cgttagattt gcttcgtttt ggcagtgcca taggtctcta
     5281 caaaaaacat atcctattcc aattccccat cccagaactg caaagctatt gatatattta
     5341 tatatatgca catatcccat attatccatt ggattatttc atataatgcc tcaagttcaa
     5401 tacagttcaa atggttttct ttaaaggttt ttagcaacat aatttttcta caagttccaa
     5461 aagataaaat attttaaaat aagcttcttg tttcagctat cgataatatc gactaaccga
     5521 ataatcgata gttttgcagc tctatccact cccataattt ttttctttga ataaaaaaaa
     5581 agtaacgaac attgtagctc atttcaaaag caatgaacgg ctgtgaattt gatggaatct
     5641 ctcaatttca atttacgcat tttggtttca tttcgtttca tttcgtttgg attctcgagc
     5701 acccttgaac ccagagaccc caatcccgga ggccccataa cccccatggc ccacagaact
     5761 cccagaccaa ccgaacgaac ttaccgcaac ccacacactt ttcacactcg acgagctcat
     5821 agaaatgcat gagctgagca aacaagaatt tgagctgggc aaacatctat tatgctccct
     5881 gaatggatgt gtccgccagc gacgtctatg gccattcaac ttaaaataat ttcgcacaat
     5941 ctcgagggct cgagcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     6001 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     6061 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6121 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6181 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6241 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6301 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6361 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6421 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6481 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6541 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6601 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6661 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6721 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6781 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     6841 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6901 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6961 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7021 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7081 atcaatctaa agtatatatg agtaaacttg gtctgacagt tagaaaaact catcgagcat
     7141 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     7201 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     7261 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     7321 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     7381 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     7441 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac
     7501 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     7561 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     7621 tgttttccca gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     7681 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     7741 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     7801 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     7861 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
     7921 ttgaatatgg ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8041 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     8101 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
