LOCUS       sequence-476075-        8330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  80X MYC tag multimer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8330)
  TITLE     Epitope tag multimer plasmids
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUC57KAN-80XMYC
FEATURES             Location/Qualifiers
     source          1..8330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1384..1403
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             1637..1666
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1679..1708
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1721..1750
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1763..1792
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1805..1834
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1865..1894
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1907..1936
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1949..1978
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1991..2020
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2033..2062
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2093..2122
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2135..2164
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2177..2206
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2219..2248
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2261..2290
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2321..2350
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2363..2392
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2405..2434
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2447..2476
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2489..2518
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2549..2578
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2591..2620
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2633..2662
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2675..2704
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2717..2746
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2777..2806
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2819..2848
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2861..2890
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2903..2932
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2945..2974
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3005..3034
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3047..3076
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3089..3118
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3131..3160
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3173..3202
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3233..3262
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3275..3304
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3317..3346
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3359..3388
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3401..3430
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3461..3490
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3503..3532
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3545..3574
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3587..3616
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3629..3658
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3689..3718
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3731..3760
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3773..3802
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3815..3844
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3857..3886
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3917..3946
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3959..3988
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4001..4030
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4043..4072
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4085..4114
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4145..4174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4187..4216
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4229..4258
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4271..4300
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4313..4342
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4373..4402
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4415..4444
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4457..4486
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4499..4528
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4541..4570
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4601..4630
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4643..4672
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4685..4714
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4727..4756
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4769..4798
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4829..4858
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4871..4900
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4913..4942
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4955..4984
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4997..5026
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5057..5086
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5099..5128
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5141..5170
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5183..5212
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5225..5254
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6154..6170)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6154..6170)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6167..6189)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6178..6194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6202..6208,6209..6226,6227..6232))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6247..6268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6385..6402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6556..7144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6636..6655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7315..8130)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7315..8130)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8038..8057
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8131..8235)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accagatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt caaaagtcat acatacacaa
      421 aaggacacgc acccacacag acccatcata gaaaccaatt tgaaattctg aatcctgctt
      481 gacgctcgtt aataaatgcc tggagcagtt tttaatgatt gctgagcttc tgtcactcat
      541 cccgtaaaat gtgtttccgg atttttgcag actgtgttca tgaactggac ggtcgccgtg
      601 gagagccatg tggactcgtc cttcttctgg ggctatctgg tgacgcagat tcccggcggc
      661 ttcatcgcct ccaagttccc ggccaacaag atattcggac tgtccatcgt gagctccgcc
      721 acgctgcatc tcttcgtgcc attcgccatg accctgatgc acggtcatgt ggtgatttgc
      781 gtgagggtcc tgcaaggact cttcgaggta tgtagcccaa ccaacctaac aacttgccca
      841 attcgatttt gccacagatc aaagccgggg attacggctg cgtgatgttg cgaaaatggg
      901 gagggttaga ttttgcttta tgataaagca aatatgtgaa atggaaacat gtcctaagcc
      961 ctcaaaggga tgggggtcct gggagggcag gaaaaaataa caaaagcatt tattttaatg
     1021 cgatttcttt gtgccaagga ctgggaagta tgtcattttt cttcgaatcg gaagggagag
     1081 aatgatactt tgtaaaaagg atgtttgtct attcgagttc cgaagtaact atattttaga
     1141 tatatatttt taatttcaca aagcacattt tcttaagata ctataactgc atgagactaa
     1201 gtgcagagtt tcattaagga ataactaatt ccctaatgaa actcgcgtcg cgccacttca
     1261 acgctcgatg ggagcgtcag cgtcgcgcca cttcaacgct cgatgggagc gtcattggtg
     1321 ggcggggtaa ccgtcgaaat cagtgtttag cccgggcagc atttttttca ctgcattcta
     1381 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccca aactcatcaa
     1441 tgtatcttat catgtctgga tcgagtttca ttaaggaata actaattccc taatgaaact
     1501 cttggaatat aggacaatat cctaattgac ttatttaaat aatccattcc tgcaggtgcc
     1561 gtcctacgat ccgcagggat accagcagca gggctccggc ggctggcgcg cccttaagct
     1621 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     1681 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     1741 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     1801 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg agggctccgg
     1861 cggcgaacag aaacttatct cagaggagga tcttggctcc ggcggcgagc aaaaactaat
     1921 atcggaggaa gacctcggtt caggagggga acaaaagctc atttctgaag aggacctggg
     1981 atctgggggt gagcaaaagc tcatatcgga agaagacctc ggatctggtg gagaacagaa
     2041 actgatttcc gaagaggatc ttggttcggg gggggtcgag ggctccggcg gcgaacagaa
     2101 acttatctca gaggaggatc ttggctccgg cggcgagcaa aaactaatat cggaggaaga
     2161 cctcggttca ggaggggaac aaaagctcat ttctgaagag gacctgggat ctgggggtga
     2221 gcaaaagctc atatcggaag aagacctcgg atctggtgga gaacagaaac tgatttccga
     2281 agaggatctt ggttcggggg gggtcgaggg ctccggcggc gaacagaaac ttatctcaga
     2341 ggaggatctt ggctccggcg gcgagcaaaa actaatatcg gaggaagacc tcggttcagg
     2401 aggggaacaa aagctcattt ctgaagagga cctgggatct gggggtgagc aaaagctcat
     2461 atcggaagaa gacctcggat ctggtggaga acagaaactg atttccgaag aggatcttgg
     2521 ttcggggggg gtcgagggct ccggcggcga acagaaactt atctcagagg aggatcttgg
     2581 ctccggcggc gagcaaaaac taatatcgga ggaagacctc ggttcaggag gggaacaaaa
     2641 gctcatttct gaagaggacc tgggatctgg gggtgagcaa aagctcatat cggaagaaga
     2701 cctcggatct ggtggagaac agaaactgat ttccgaagag gatcttggtt cggggggggt
     2761 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     2821 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     2881 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     2941 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg agggctccgg
     3001 cggcgaacag aaacttatct cagaggagga tcttggctcc ggcggcgagc aaaaactaat
     3061 atcggaggaa gacctcggtt caggagggga acaaaagctc atttctgaag aggacctggg
     3121 atctgggggt gagcaaaagc tcatatcgga agaagacctc ggatctggtg gagaacagaa
     3181 actgatttcc gaagaggatc ttggttcggg gggggtcgag ggctccggcg gcgaacagaa
     3241 acttatctca gaggaggatc ttggctccgg cggcgagcaa aaactaatat cggaggaaga
     3301 cctcggttca ggaggggaac aaaagctcat ttctgaagag gacctgggat ctgggggtga
     3361 gcaaaagctc atatcggaag aagacctcgg atctggtgga gaacagaaac tgatttccga
     3421 agaggatctt ggttcggggg gggtcgaggg ctccggcggc gaacagaaac ttatctcaga
     3481 ggaggatctt ggctccggcg gcgagcaaaa actaatatcg gaggaagacc tcggttcagg
     3541 aggggaacaa aagctcattt ctgaagagga cctgggatct gggggtgagc aaaagctcat
     3601 atcggaagaa gacctcggat ctggtggaga acagaaactg atttccgaag aggatcttgg
     3661 ttcggggggg gtcgagggct ccggcggcga acagaaactt atctcagagg aggatcttgg
     3721 ctccggcggc gagcaaaaac taatatcgga ggaagacctc ggttcaggag gggaacaaaa
     3781 gctcatttct gaagaggacc tgggatctgg gggtgagcaa aagctcatat cggaagaaga
     3841 cctcggatct ggtggagaac agaaactgat ttccgaagag gatcttggtt cggggggggt
     3901 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     3961 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     4021 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     4081 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg agggctccgg
     4141 cggcgaacag aaacttatct cagaggagga tcttggctcc ggcggcgagc aaaaactaat
     4201 atcggaggaa gacctcggtt caggagggga acaaaagctc atttctgaag aggacctggg
     4261 atctgggggt gagcaaaagc tcatatcgga agaagacctc ggatctggtg gagaacagaa
     4321 actgatttcc gaagaggatc ttggttcggg gggggtcgag ggctccggcg gcgaacagaa
     4381 acttatctca gaggaggatc ttggctccgg cggcgagcaa aaactaatat cggaggaaga
     4441 cctcggttca ggaggggaac aaaagctcat ttctgaagag gacctgggat ctgggggtga
     4501 gcaaaagctc atatcggaag aagacctcgg atctggtgga gaacagaaac tgatttccga
     4561 agaggatctt ggttcggggg gggtcgaggg ctccggcggc gaacagaaac ttatctcaga
     4621 ggaggatctt ggctccggcg gcgagcaaaa actaatatcg gaggaagacc tcggttcagg
     4681 aggggaacaa aagctcattt ctgaagagga cctgggatct gggggtgagc aaaagctcat
     4741 atcggaagaa gacctcggat ctggtggaga acagaaactg atttccgaag aggatcttgg
     4801 ttcggggggg gtcgagggct ccggcggcga acagaaactt atctcagagg aggatcttgg
     4861 ctccggcggc gagcaaaaac taatatcgga ggaagacctc ggttcaggag gggaacaaaa
     4921 gctcatttct gaagaggacc tgggatctgg gggtgagcaa aagctcatat cggaagaaga
     4981 cctcggatct ggtggagaac agaaactgat ttccgaagag gatcttggtt cggggggggt
     5041 cgagggctcc ggcggcgaac agaaacttat ctcagaggag gatcttggct ccggcggcga
     5101 gcaaaaacta atatcggagg aagacctcgg ttcaggaggg gaacaaaagc tcatttctga
     5161 agaggacctg ggatctgggg gtgagcaaaa gctcatatcg gaagaagacc tcggatctgg
     5221 tggagaacag aaactgattt ccgaagagga tcttggttcg gggggggtcg acggatccgc
     5281 ggccgcataa tgggttcgcg ataggtcact gatatatatc atagctttta gttgtagtcg
     5341 gaaatcgttt gacgtttatt gtttgttctc ggtgtgccgt taaagttttc cccccttttt
     5401 ctctctattt gttgagttcc atcgatggac ggatccgtgg acctttcaga agtatataaa
     5461 tatataacta aagagtaacg aatttgtcag cgaataaggg gattccacat gccacactaa
     5521 cacaatgaaa ctatgtacct aacagagaaa ctacatatgc atacacagca ataccgataa
     5581 ccactaccaa taccattagc ccacatagac ccaaactggc ctaatcgatc tataaagcat
     5641 atatagtata tactattagg gtctcgctta cgcgaatttc tatttacaag ctccgaaatc
     5701 gaaactgcaa cacaagatag ggaaagaact ggaaatatat actcaacact tgtgtatatc
     5761 ataaatcaca taatccacac aaagcctatg ataattatat ttatatcggt ataatcgtat
     5821 atacatatat agccaaagat tgttgttgaa ctgcgaactg caagttttat taccgttgat
     5881 gatattatct tctgtgtaaa gagaacattt taatcaaacc caagcaattg ttgatgttcg
     5941 gcacgaatga taaccataaa aagaactagc taatcaaaag tcatatgaat actctgttaa
     6001 ctaattactg tttatgtttg ttgatattat ttaattcatt gatattaatg caccgtatgc
     6061 ttttgtgttt aactaacatg agcaaggcat gatgtaggta ctaaaaataa tacttaagta
     6121 gatagccgta gaagcttggc tcgatcgagc atggtcatag ctgtttcctg tgtgaaattg
     6181 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6241 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6301 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6361 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6421 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6481 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6541 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6601 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6661 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6721 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6781 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6841 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     6901 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     6961 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7021 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7081 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7141 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7201 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7261 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttagaa
     7321 aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata
     7381 tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat
     7441 ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa
     7501 tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc
     7561 cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt
     7621 acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg
     7681 agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa
     7741 ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc
     7801 taatacctgg aatgctgttt tcccagggat cgcagtggtg agtaaccatg catcatcagg
     7861 agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct
     7921 gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc
     7981 tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc
     8041 gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga
     8101 gcaagacgtt tcccgttgaa tatggctcat actcttcctt tttcaatatt attgaagcat
     8161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8281 tatcatgaca ttaacctata aaaataagcg tatcacgagg ccctttcgtc
//
