LOCUS       sequence-47609-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by 
            alternative lox FAS sites), for optogenetic excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(175..198)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(373..392)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     642..660
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattcac aacttcgtat
       61 agaaaggtat atacgaagtt gtgctagctt acttgtacag ctcgtccatg ccgtacagga
      121 acaggtggtg gcggccctcg gagcgctcgt actgttccac gatggtgtag tcctcgttgt
      181 gggaggtgat gtccagcttg gtgtccacgt agtagtagcc gggcagttgc acgggcttct
      241 tggccatgta gatggtcttg aactccacca ggtagtggcc gccgtccttc agcttcaggg
      301 cctggtggat ctcgcccttc agcacgccgt cgcgggggta caggcgctcg gtggaggcct
      361 cccagcccat ggtcttcttc tgcattacgg ggccgtcggg ggggaagttg gtgccgcgca
      421 tcttcacctt gtagatcagc gtgccgtcct gcagggagga gtcctgggtc acggtcacca
      481 gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
      541 tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc cttggagccg tacatgaact
      601 ggggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagcttgg
      661 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
      721 cgttcatgga gccctccatg cgcaccttga agcgcatgaa ctctttgatg acggccatgt
      781 tgttgtcctc
//
