LOCUS       sequence-476093-        9429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A...
FEATURES             Location/Qualifiers
     source          1..9429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     584..603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(822..926)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(952..1407)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1089..1110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1301..1320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1534..1556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1548..1565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1549..1565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1695..1757
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2340..3518
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2571..3509
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3466..3486
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3527..3536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3542..3544,3545..3865)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     misc_feature    join(5204..5778,5779..5781,5782..5790)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5371..5388)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5598..5617
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5791..6387
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5791..5810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6287..6307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6388..6444
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6451..7161
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(7195..7212)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7201..7408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   7704..7738
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8229..8245)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8229..8245)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8242..8264)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8253..8269
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8277..8283,8284..8301,8302..8307))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8322..8343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8460..8477)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8631..9219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8711..8730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9390..9429,1..752,753..821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
       61 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
      961 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1021 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1081 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1141 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1201 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     1261 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1321 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1381 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     1441 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1501 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1561 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     1621 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     1681 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     1741 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     1801 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     1861 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     1921 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     1981 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     2041 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     2101 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     2161 aaaaggcctc cacggccact agtttcgcac gattgcacct tgggagcctt aaccgctttt
     2221 gaatgtccgt cttagttaat ccctaattga acgctccaaa aggtgacttc caatagggat
     2281 ttattccttt taaaattaac ggcataatcg tgccgccgaa tgcaacctcg atcgagccgg
     2341 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2401 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2461 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2521 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     2581 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     2641 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     2701 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     2761 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     2821 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     2881 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     2941 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3001 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3061 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3121 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3181 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3241 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3301 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3361 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3421 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     3481 tggttcaaag tttttttctt ccatttcagg tgtcgtgaat ctagaagcca ccatggctag
     3541 catgggagtg caggtggaaa ccatctcccc aggagacggg cgcaccttcc ccaagcgcgg
     3601 ccagacctgc gtggtgcact acaccgggat gcttgaagat ggaaagaaat ttgattcctc
     3661 ccgggacaga aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg
     3721 ggaagaaggg gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga
     3781 ttatgcctat ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt
     3841 cgatgtggag cttctaaaac tggaaggcgc cgggacagga gggtctgggg gtggcactgg
     3901 cggatctgga ggagactaca aggacgatga cgacaacggc ggatcggggg ggtctggtgg
     3961 tagtggcggt tcggggggag gtaccggttt gcacttggat caaacgccat ctcgccagcc
     4021 tattcccagt gagggtcttc agctgcattt accgcaggtt ttagctgacg ctgtctcacg
     4081 cctggtcctg ggtaagtttg gtgacctgac cgacaacttc tcctcccctc acgctcgcag
     4141 aaaagtgctg gctggagtcg tcatgacaac aggcacagat gttaaagatg ccaaggtgat
     4201 aagtgtttct acaggaacaa aatgtattaa tggtgaatac atgagtgatc gtggccttgc
     4261 attaaatgac tgccatgcag aaataatatc tcggagatcc ttgctcagat ttctttatac
     4321 acaacttgag ctttacttaa ataacaaaga tgatcaaaaa agatccatct ttcagaaatc
     4381 agagcgaggg gggtttaggc tgaaggagaa tgtccagttt catctgtaca tcagcacctc
     4441 tccctgtgga gatgccagaa tcttctcacc acatgagcca atcctggaag aaccagcaga
     4501 tagacaccca aatcgtaaag caagaggaca gctacggacc aaaatagagt ctggtcaggg
     4561 gacgattcca gtgcgctcca atgcgagcat ccaagcgtgg gacggggtgc tgcaagggga
     4621 gcggctgctc accatgtcct gcagtgacaa gattgcacgc tggaacgtgg tgggcatcca
     4681 gggatccctg ctcagcattt tcgtggagcc catttacttc tcgagcatca tcctgggcag
     4741 cctttaccac ggggaccacc tttccagggc catgtaccag cggatctcca acatagagga
     4801 cctgccacct ctctacaccc tcaacaagcc tttgctcagt ggcatcagca atgcagaagc
     4861 acggcagcca gggaaggccc ccaacttcag tgtcaactgg acggtaggcg actccgctat
     4921 tgaggtcatc aacgccacga ctgggaagga tgagctgggc cgcgcgtccc gcctgtgtaa
     4981 gcacgcgttg tactgtcgct ggatgcgtgt gcacggcaag gttccctccc acttactacg
     5041 ctccaagatt accaaaccca acgtgtacca tgagtccaag ctggcggcaa aggagtacca
     5101 ggccgccaag gcgcgtctgt tcacagcctt catcaaggcg gggctggggg cctgggtgga
     5161 gaagcccacc gagcaggacc agttctcact cacgccctga tagcccctct ccctcccccc
     5221 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5281 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5341 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5401 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5461 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5521 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5581 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5641 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     5701 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     5761 gaaaaacacg atgataatat ggccacaacc atgaccgagt acaagcccac ggtgcgcctc
     5821 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5881 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5941 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6001 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6061 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6121 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc
     6181 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6241 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6301 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6361 tggtgcatga cccgcaagcc cggtgccgcc acgaacttct ctctgttaaa gcaagcaggt
     6421 gacgtggaag aaaaccccgg tcccggatcc atggtgtcta agggcgaaga gctgattaag
     6481 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     6541 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     6601 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     6661 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     6721 ggcttcacat gggagagagt caccacatac gaggacgggg gcgtgctgac cgctacccag
     6781 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     6841 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgaaacg
     6901 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     6961 gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac
     7021 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     7081 aacgaaacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
     7141 aaactggggc acaaacttaa ttaagaattc ctcgagctag agctcgctga tcagcctcga
     7201 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7261 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7321 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7381 gggaagacaa tagcaggcat gctggggacg acgtcaggtg gcacttttcg atatctataa
     7441 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
     7501 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
     7561 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
     7621 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
     7681 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
     7741 aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag
     7801 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc
     7861 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac
     7921 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt
     7981 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag
     8041 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt
     8101 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg
     8161 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg
     8221 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8281 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8401 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     8461 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     8521 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     8581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     8641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     8701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     8761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     8821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     8881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     8941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     9001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     9061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     9121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     9181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     9241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     9301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     9361 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     9421 tcagcgatc
//
