LOCUS       sequence-476094-       12309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and 
            ARRB2-tdMCP separated by EMCV IRES.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Zhang X, Mille-Fragoso LS, Kaseniit KE, Lee AP, Zhang M, Call CC, Hu
            Y, Xie Y, Gao XJ
  TITLE     Post-transcriptional modular synthetic receptors.
  JOURNAL   Nat Chem Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w.
  PUBMED    40155716
REFERENCE   2  (bases 1 to 12309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2025 Mar 28. doi: 10.1038/s41589-025-01872-w."
COMMENT     Sequence Label: 
            XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..12309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     584..603
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(822..926)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(952..1407)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1089..1110)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1301..1320
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1534..1556
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1548..1565
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1549..1565
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1695..1757
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        2340..3518
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2571..3509
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3466..3486
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    join(6050..6624,6625..6627,6628..6636)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6217..6234)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6444..6463
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8611..8664
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8671..9267
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8671..8690)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9167..9187
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             9268..9324
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             9331..10041
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(10075..10092)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10081..10288
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   10584..10618
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11109..11125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11109..11125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11122..11144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11133..11149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11157..11163,11164..11181,11182..11187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11202..11223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11340..11357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11511..12099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11591..11610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12270..12309,1..752,753..821))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
       61 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
      121 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      181 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      241 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      301 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      361 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      421 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      481 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      541 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      601 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
      661 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
      721 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
      781 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      841 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      901 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
      961 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     1021 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     1081 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     1141 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     1201 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     1261 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     1321 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     1381 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     1441 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     1501 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     1561 ccagtgagcg cgcctcgttc attcacgttt ttgaacccgt ggaggacggg cagactcgcg
     1621 gtgcaaatgt gttttacagc gtgatggagc agatgaagat gctcgacacg ctgcagaaca
     1681 cgcagctaga ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg
     1741 tgtaaaattg acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa
     1801 tagatattaa gttttattat atttacactt acatactaat aataaattca acaaacaatt
     1861 tatttatgtt tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa
     1921 caaaactttt atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc
     1981 ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg
     2041 catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg
     2101 ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg
     2161 aaaaggcctc cacggccact agtttcgcac gattgcacct tgggagcctt aaccgctttt
     2221 gaatgtccgt cttagttaat ccctaattga acgctccaaa aggtgacttc caatagggat
     2281 ttattccttt taaaattaac ggcataatcg tgccgccgaa tgcaacctcg atcgagccgg
     2341 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     2401 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     2461 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     2521 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     2581 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     2641 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
     2701 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
     2761 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
     2821 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
     2881 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
     2941 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     3001 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     3061 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     3121 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     3181 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     3241 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     3301 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     3361 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     3421 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     3481 tggttcaaag tttttttctt ccatttcagg tgtcgtgaat ctagaagcca ccatgaagac
     3541 gatcatcgcc ctgagctaca tcttctgcct ggtattcgcc gactacaagg acgatgatga
     3601 cgccagcatc gatatgagcg gagagagcat gaacttcagt gatgtgttcg attccagtga
     3661 ggactacttc gtgtccgtca atacatccta ctacagcgta gattctgaga tgctgctttg
     3721 ctccctgcag gaggtgcgcc agttctcccg actcttcgtt cccatcgcgt attcactcat
     3781 ctgcgtcttt ggcctcctgg ggaatatctt ggtagtcatc acatttgctt tttataaaaa
     3841 ggccagatcc atgacagacg tctacctgct taatatggcc attgcagaca tcctgttcgt
     3901 cctgacgctc ccattctggg ctgtgagtca cgctaccggg gcctgggtgt tcagtaatgc
     3961 aacctgcaag ctgttgaagg gaatttatgc catcaacttt aattgcggta tgctgctgct
     4021 gacttgcata agtatggaca gatacatcgc catagtacag gcaacaaaat ccttcagact
     4081 gaggtctcga acgctcccta ggtctaaaat catttgtctt gttgtatggg gcttgagcgt
     4141 gattatttcc agcagtactt tcgtgtttaa ccagaagtac aacacgcagg gcagcgatgt
     4201 gtgtgagccc aagtaccaga cggtatctga gcctatcagg tggaagctgt tgatgctggg
     4261 tttggaattg ctgttcggct tttttatccc actcatgttt atgatttttt gttatacctt
     4321 catcgtgaag accctggtcc aggcacaaaa ctctaagcga cacaaggcca tccgggtaat
     4381 catcgctgtt gtgctggtat ttctggcttg ccagatccct cataatatgg tccttctggt
     4441 aacggcagcc aacctgggca aaatgaatcg ctcatgtcag tcagaaaagc ttatcgggta
     4501 caccaaaacg gttacggagg ttctggcgtt cctgcattgt tgccttaacc ctgtgctgta
     4561 cgcatttata ggtcagaagt ttcgaaacta cttcctgaag attctcaaag acctctggtg
     4621 tgtaagaaga aagtataaaa gtagtggatt ttcatgcgca ggacgctact ccgaaaacat
     4681 ttccagacag accagtgaga cggccgataa cgataacgcc tcctccttta ccatgaccgg
     4741 tggacgcacc ccacccagcc tgggtcccca agatgagtcc tgcaccaccg ccagctcctc
     4801 cctggccaag gacacttcat cgggtaccgg tgctttgcac ttggatcaaa cgccatctcg
     4861 ccagcctatt cccagtgagg gtcttcagct gcatttaccg caggttttag ctgacgctgt
     4921 ctcacgcctg gtcctgggta agtttggtga cctgaccgac aacttctcct cccctcacgc
     4981 tcgcagaaaa gtgctggctg gagtcgtcat gacaacaggc acagatgtta aagatgccaa
     5041 ggtgataagt gtttctacag gaacaaaatg tattaatggt gaatacatga gtgatcgtgg
     5101 ccttgcatta aatgactgcc atgcagaaat aatatctcgg agatccttgc tcagatttct
     5161 ttatacacaa cttgagcttt acttaaataa caaagatgat caaaaaagat ccatctttca
     5221 gaaatcagag cgaggggggt ttaggctgaa ggagaatgtc cagtttcatc tgtacatcag
     5281 cacctctccc tgtggagatg ccagaatctt ctcaccacat gagccaatcc tggaagaacc
     5341 agcagataga cacccaaatc gtaaagcaag aggacagcta cggaccaaaa tagagtctgg
     5401 tcaggggacg attccagtgc gctccaatgc gagcatccaa gcgtgggacg gggtgctgca
     5461 aggggagcgg ctgctcacca tgtcctgcag tgacaagatt gcacgctgga acgtggtggg
     5521 catccaggga tccctgctca gcattttcgt ggagcccatt tacttctcga gcatcatcct
     5581 gggcagcctt taccacgggg accacctttc cagggccatg taccagcgga tctccaacat
     5641 agaggacctg ccacctctct acaccctcaa caagcctttg ctcagtggca tcagcaatgc
     5701 agaagcacgg cagccaggga aggcccccaa cttcagtgtc aactggacgg taggcgactc
     5761 cgctattgag gtcatcaacg ccacgactgg gaaggatgag ctgggccgcg cgtcccgcct
     5821 gtgtaagcac gcgttgtact gtcgctggat gcgtgtgcac ggcaaggttc cctcccactt
     5881 actacgctcc aagattacca aacccaacgt gtaccatgag tccaagctgg cggcaaagga
     5941 gtaccaggcc gccaaggcgc gtctgttcac agccttcatc aaggcggggc tgggggcctg
     6001 ggtggagaag cccaccgagc aggaccagtt ctcactcacg ccctgatagc ccctctccct
     6061 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     6121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     6181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     6241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     6301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     6361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     6421 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     6481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta
     6541 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     6601 tcctttgaaa aacacgatga taatatggcc acaaccatgg gggagaaacc cgggaccagg
     6661 gtcttcaaga agtcgagccc taactgcaag ctcaccgtgt acttgggcaa gcgggacttc
     6721 gtagatcacc tggacaaagt ggaccctgta gatggcgtgg tgcttgtgga ccctgactac
     6781 ctgaaggacc gcaaagtgtt tgtgaccctc acctgcgcct tccgctatgg ccgtgaagac
     6841 ctggatgtgc tgggcttgtc cttccgcaaa gacctgttca tcgccaccta ccaggccttc
     6901 cccccggtgc ccaacccacc ccggcccccc acccgcctgc aggaccggct gctgaggaag
     6961 ctgggccagc atgcccaccc cttcttcttc accatacccc agaatcttcc atgctccgtc
     7021 acactgcagc caggcccaga ggatacagga aaggcctgcg gcgtagactt tgagattcga
     7081 gccttctgtg ctaaatcact agaagagaaa agccacaaaa ggaactctgt gcggctggtg
     7141 atccgaaagg tgcagttcgc cccggagaaa cccggccccc agccttcagc cgaaaccaca
     7201 cgccacttcc tcatgtctga ccggtccctg cacctcgagg cttccctgga caaggagctg
     7261 tactaccatg gggagcccct caatgtaaat gtccacgtca ccaacaactc caccaagacc
     7321 gtcaagaaga tcaaagtctc tgtgagacag tacgccgaca tctgcctctt cagcaccgcc
     7381 cagtacaagt gtcctgtggc tcaactcgaa caagatgacc aggtatctcc cagctccaca
     7441 ttctgtaagg tgtacaccat aaccccactg ctcagcgaca accgggagaa gcggggtctc
     7501 gccctggatg ggaaactcaa gcacgaggac accaacctgg cttccagcac catcgtgaag
     7561 gagggtgcca acaaggaggt gctgggaatc ctggtgtcct acagggtcaa ggtgaagctg
     7621 gtggtgtctc gaggcgggga tgtctctgtg gagctgcctt ttgttcttat gcaccccaag
     7681 ccccacgacc acatccccct ccccagaccc cagtcagccg ctccggagac agatgtccct
     7741 gtggacacca acctcattga atttgatacc aactatgcca cagatgatga cattgtgttt
     7801 gaggactttg cccggcttcg gctgaagggg atgaaggatg acgactatga tgatcaactc
     7861 tgcggcggtg gtagcgctat ggcgagcaac tttacgcaat ttgtgttggt tgacaatggt
     7921 ggcaccggag acgtaactgt tgcgccgagc aattttgcga acgggattgc agaatggata
     7981 agtagtaaca gccggagtca ggcatataaa gtcacatgct cagttcgcca aagttccgca
     8041 cagaaccgaa aatataccat aaaggtagaa gtgccaaaag gggcgtggcg atcttatctt
     8101 aatatggagc ttacgatccc aatctttgcg actaattcag actgcgagct gattgtcaag
     8161 gccatgcaag gtcttttgaa agacggtaac ccgattccct ctgcgatcgc cgccaactca
     8221 ggaatatatg gtggcggaac cggtggcggg tccatggcct ccaattttac acagtttgtg
     8281 ctcgtggaca acggcggcac tggagacgtc acagtagctc caagtaactt tgcgaatggg
     8341 atagccgagt ggatctcttc caattcaaga tcacaagcct ataaggtgac ttgtagtgta
     8401 cgccaatcta gtgcccagaa ccggaagtat accattaaag tggaggtccc caaaggtgca
     8461 tggcgctcat acttgaatat ggaattgacg attccgatat ttgcgaccaa tagcgactgc
     8521 gagcttattg taaaagccat gcaaggactt ctgaaagatg gcaacccgat accttccgca
     8581 atcgctgcga atagcggaat ttacgctagc gaaggtcggg ggagtttgct tacctgtggc
     8641 gatgtcgagg agaatccagg gcccaagctt atgaccgagt acaagcccac ggtgcgcctc
     8701 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     8761 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     8821 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     8881 gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     8941 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     9001 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggagtc
     9061 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     9121 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     9181 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     9241 tggtgcatga cccgcaagcc cggtgccgcc acgaacttct ctctgttaaa gcaagcaggt
     9301 gacgtggaag aaaaccccgg tcccggatcc atggtgtcta agggcgaaga gctgattaag
     9361 gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca cttcaagtgc
     9421 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     9481 gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct ctacggcagc
     9541 aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc cttccctgag
     9601 ggcttcacat gggagagagt caccacatac gaggacgggg gcgtgctgac cgctacccag
     9661 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     9721 acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt caccgaaacg
     9781 ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa gctcgtgggc
     9841 gggagccatc tgatcgcaaa cgccaagacc acatatagat ccaagaaacc cgctaagaac
     9901 ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa ggaggccaac
     9961 aacgaaacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga cctccctagc
    10021 aaactggggc acaaacttaa ttaagaattc ctcgagctag agctcgctga tcagcctcga
    10081 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10141 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10201 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10261 gggaagacaa tagcaggcat gctggggacg acgtcaggtg gcacttttcg atatctataa
    10321 caagaaaata tatatataat aagttatcac gtaagtagaa catgaaataa caatataatt
    10381 atcgtatgag ttaaatctta aaagtcacgt aaaagataat catgcgtcat tttgactcac
    10441 gcggtcgtta tagttcaaaa tcagtgacac ttaccgcatt gacaagcacg cctcacggga
    10501 gctccaagcg gcgactgaga tgtcctaaat gcacagcgac ggattcgcgc tatttagaaa
    10561 gagagagcaa tatttcaaga atgcatgcgt caattttacg cagactatct ttctagggtt
    10621 aatctagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag
    10681 ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc
    10741 ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac
    10801 gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt
    10861 tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag
    10921 cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt
    10981 gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg
    11041 ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcttggcg
    11101 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
    11161 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    11221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    11281 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
    11341 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
    11401 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
    11461 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    11521 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    11581 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    11641 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    11701 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    11761 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    11821 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    11881 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    11941 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    12001 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    12061 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    12121 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    12181 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    12241 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    12301 tcagcgatc
//
