LOCUS       sequence-47611-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, 
            expresses EGFP in Cre positive cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(102..125)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(300..319)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     569..587
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gagctgtaca agtaattact tgtacagctc gtccatgccg tacaggaaca ggtggtggcg
       61 gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
      121 cagcttggtg tccacgtagt agtagccggg cagttgcacg ggcttcttgg ccatgtagat
      181 ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct ggtggatctc
      241 gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc agcccatggt
      301 cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta
      361 gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc
      421 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct tgtaatcggg
      481 gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac atgaactggg gggacaggat
      541 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
      601 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcatggagcc
      661 ctccatgcgc accttgaagc gcatg
//
