LOCUS       sequence-476119-       15978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This all-in-one subcellar localization vector is used to study the 
            nuclear localization of AtbZIP63.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 15978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTCL1-AtbZIP63
FEATURES             Location/Qualifiers
     source          1..15978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1164..1874)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1442..1461)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2323..2668)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2698..2872
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(2880..3593,3594..3596,3597..3599))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2883..3593,3594..3596,3597..3599))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2925..2946)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3274..3293
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3533..3554
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(4616..5292)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(9064..9081)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9064..9080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9073..9095)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    9283..9307
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10608..11237
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11666..12739
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12805..12999
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13235..13257)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13343..13483
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13398..13417
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13498..13515)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13669..14257)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13749..13768)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14344..15138)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15563..15587
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    15855..15876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15891..15896,15897..15914,15915..15921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15929..15945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15934..15956
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15953..15969
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15953..15969
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcggtaccct agattacttg tacagctcgt ccatgccgcc ggtggagtgg
     1201 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
     1261 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
     1321 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
     1381 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
     1441 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
     1501 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
     1561 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
     1621 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
     1681 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
     1741 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
     1801 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg
     1861 cccttgctca ccatggatcc agaactcgta aacttcgtaa ccgccttagt cccttcagaa
     1921 acagcatgtt tcgccaactc tccaggcaac acaagtctca ccgcagtctg aatctcccta
     1981 gaagtaatcg tcggcttctt gttgtacctc gcaagcttcg aagactcacc agcaagtttc
     2041 tcaaagatat cattgatgaa actgttcatg attcccatgg ctttgctgga gattccgata
     2101 tctggatgaa cttgcttcaa caccttgaag atgtagatct tgtatgtctc aacgttcttc
     2161 tttgatctct tcttcttctt gtctccggcg ccggctggtt ccttcgggag tttctttccg
     2221 gcttttggtt tcttctctgc cgctgctgct ggttcggctg ccggagtttt ctctgccggt
     2281 ttcttctccg ctggtttctt atctgccttc gccatggtac cctgtcctct ccaaatgaaa
     2341 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     2401 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     2461 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     2521 atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
     2581 ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga ggaggtttcc
     2641 ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga ctgtatcgat
     2701 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
     2761 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
     2821 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct
     2881 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     2941 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     3001 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     3061 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     3121 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     3181 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     3241 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     3301 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     3361 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     3421 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     3481 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     3541 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatc
     3601 tgatccccaa cgcttcgaat acgtttctgc agatgttcca agctctcaac tctacgcatc
     3661 gaagctgttc tgttcatctt gcaccctatc aaggccttgc ctttgttccc cgagctaatg
     3721 atctctggtg tagtcacttg gctgcttgta gtgtctggag aatttgatgt ctctgaagga
     3781 agagagacag ttgaaacaat ctgaggcata ttgtggaaca ttgggttaaa gccagtgagt
     3841 ctcttcactg tctcttcagc cattttcacc tttgctcgta gtgtctcaat attggctttt
     3901 aaaactctgt tttctacaga tgcatcattg aatgtttgag ttacatcagt gagacccttc
     3961 atgagttttg aattctctac acgaagctgt gaaacttgtg tctctagctc actcaagtgt
     4021 gcttgctttc ttcttctgga ccgtctagct gattctctat tagagagcat ccttttaacg
     4081 cgtttaacat tagtagggtt catattagtt tcaccatcag cttcttcttc atcaccagag
     4141 agctcagatc cacttgttat agcactgctc atcattggtg tagctttgga agaagcaaga
     4201 gaggcttgtt ctgattcatt ggctccacca ttgtcagatc taccagaagt atcctgaggt
     4261 ttgatgaaag ttcccctttt catggcgaca gcagcacaag caagattaag tttactcttg
     4321 agaaatgctc tgtattcctc tgaatctaca ggaacattag gaggagagga gacggaaaca
     4381 ccacacgccg tcgtagattc tccgtcgtct gcagcggcgg aggattcttg tatgaaacga
     4441 ttgaatgccc attcggaagc actgcgattc aacgacgtca ttccattaac cgaccagtga
     4501 tggttaccgg agatttcttc gtcggagaaa actttttcca tggtctcgga tctcattgcc
     4561 ccccggatct gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc
     4621 gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
     4681 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
     4741 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     4801 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     4861 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     4921 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     4981 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     5041 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     5101 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
     5161 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
     5221 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
     5281 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
     5341 acgagagtgt cgtgctccac catgttggca agctgctcta gctcacgttg acggaaactg
     5401 cagaacgatc tcagttaggt catgggaaag ctgatattca tccctctgag actctatata
     5461 attaaatgcg tttgggggat tagctaattg agaatccata tagagaaata tggccgcgca
     5521 gcgacgccgg ttgaagaata tgaacaagga ataagatgaa caaggaagaa gatgaacaag
     5581 caataattta attaattatt gttaataagc ataaattatg aaaataattt tgatggtaat
     5641 gaaagtatta taaataatat ttaggagaaa atattgttaa ggaaatatct ggttatgttt
     5701 atttgttaag gtatatgggt ttttatagaa acccaggagc agctatggta agaaacccag
     5761 gactcaaaaa atgcctctca acttctctca tatgttgcgg agtctggagt cccatttata
     5821 ccatagaaag cattgggggt atagcatggt aatttcacat cggactccat accgcggcca
     5881 tctatataat attactgatg gccgcgcgaa gtcccggtat gccttttcag ccgttggatc
     5941 tggagccgta cctttttcgc gttcccattt tgcccctgga gtcccgagct atttacgacc
     6001 ccctgccacc gagtcccctt cgtggagcgc tcttcgtcca acacgtgtcc caacttttgc
     6061 gcatttttga ctacctgtcg cgcacttgaa ttaaagttgc ccttttattt tcttccaatt
     6121 attttgcggc tgagtagtct agtggatccc ccggagctcc acttgggata ggttaagaaa
     6181 atatgtctgg cttgtaaacg aaaagaatcg gggtttctag gcatttttta aatagtgacc
     6241 caggactcaa aaactagctc tcaacttctc tcatatgttg tggagtctgg agtcctttat
     6301 atatgtttac gctatagaag gcatttgggg actctcccgg gcccaccata ggactccata
     6361 ccgcggccat ctatataata ttactgatgg ccgcgcggat tttggagccc ccttttgtcg
     6421 ttagcgcgac ctttaattca aattaaaggt tgcttttttc tatcgtccga tcattttgcg
     6481 cctgactagc ctagatatta gcaacaactt cgcggctaag ttgttgaccg tcaagtataa
     6541 attaatgggc ttatggccta atgggtatag agcaaaatgg catgcctgca gactagtgct
     6601 ttactctgat ccgcagatat ttcatcagga aagagctttg caagttcctt cattgtagca
     6661 tcaataattt ctgagtcact acatgcaatc cattcttctg ctggggcaaa aactaactcc
     6721 aacatggatt ggtttggatt gtaatattcc ttacatgtta cagacatgtc agcataaaca
     6781 cttaaaagcg ggcttctgct aaagagtaga tgatcatagg tgttcttcaa tttcctatca
     6841 aaccagatgt gaacgttgat aactggaact ccaactaatt tctctaattt cttgaagtat
     6901 gaaatctctc tccagtcttc aggcactagt gaattcgcta gcgttaactg gccataatcc
     6961 tgtgtatgca acgttgaaaa ttcggatcta tttttacgat tcgatcatga attaataaaa
     7021 tttaaatttt attgaatgat tttctagtac ataactgaca tacgacttat cagttgcgaa
     7081 acgaacagct ctaattacat tgttaagtga aattacgcct aattcgtcta agtacaacat
     7141 aaccaactgt ttaaacctaa ttaaataagt cgacccagaa gctgtcagag atgtcgtcca
     7201 gacttggaag ttcaggtaag ctttgtggag atgcaacgct ctccgtaagt tgtggttgaa
     7261 tcggatctgg acgtggtata tacgcgtcct ggtgtatagc agatcctcta ctccgtatat
     7321 cttgaaatat aggggattta ttatctccca gatatacacg ccattctctg cctgacgtgc
     7381 agtgatgatt tcccctgtgc gtgaatccat gaccggtgca gcctatgtgg aagtatatgg
     7441 tgcacccaca gtttagatca atgcgtcgtc gtctaatcgc cctcctcttg gcttgcctgt
     7501 gtgccttctt gatagagggg ggcgtcgaga gtgatgaaga taacattctt gagagtccaa
     7561 tgtttgagcg acgcattttc cgctttatcc aggaaatctt tatagctgga tccctcgcct
     7621 ggattgcaaa gcacgattga agggatccct cctttaattt gaactggttt cccgtacttg
     7681 caatttgact gccagtcttt ttgggcccca agaagctctt tccaatgctt tagctttagg
     7741 tattgcggtg ctacgtcatc aatgacgtta taggcaacat tattcgagta cactcgatta
     7801 ttgaagtcca ggtgtccact gaggtaatta tgtgggccta aagcacgagc ccacatcgtc
     7861 ttgcccgttc tagaatcacc ttccactatg agactaactg gtcttacagg ccgcgcagcg
     7921 ggacccattc caaaataatc atccgcccat tcttgcatct gttcaggaac gttagtgaaa
     7981 gaggaaagtg gaaatggagg gacccattgt tcaggcgcct ttttgaaaag gcgttccaag
     8041 ttcgctgtaa tgttatggta actcacgatg aacgcccttg gatctccggc ccggataatt
     8101 gcaagagctt ccgccaaact aggtgcattg atggcgttat aatagacgtc gtctttattc
     8161 ttctttgtac ccccaggcac tttgaactgt ccggattcac aacaatcgcc gtctttggtg
     8221 atgtaattct tgacggcgtt ggcgtctttg gctgcttgta tgtttgggtg aaaattggtc
     8281 gaccttctgg ggtgagtaag gtcgaaaaat ctcttattct tgatgttgga ctttccagag
     8341 agttggatga gacagtgaag gtgtgggaat ccgtccgagt gctcctctct ggcaactcga
     8401 atatatgttg gtttgacgac tgaccatgat aaggtttgaa gcatttgaag agcttcattt
     8461 tttgggatat cgcacttggg ataggttaag aaaatatgtc tggcttgtaa acgaaaagaa
     8521 tcggggtttc taggcatttt ttaaatagtg acccaggact caaaaactag ctctcaactt
     8581 ctctcatatg ttgtggagtc tggagtcctt tatatatgtt tacgccatag aaagcattgg
     8641 gggtatngca tggtaatttc atatcggact ccataccgcg gccatctata taatattact
     8701 gatggccgcg cggattttgg agcccccttt tgtcgttagc gcgaccttta attcaaatta
     8761 aaggttgctt ttttctatcg tccaatcatt ttgcgcctga ctagcctaga tattagcaac
     8821 aacttcgcga ctaagttgtt gaccgtcaag tataaattaa tgggcttatg gcccaatggg
     8881 tatagagcaa aatgcccaag cgtgatgccc cgtggcgctt attagcgggt acgtctaagg
     8941 taagtcgaaa tgccaactac tctcccttga gagtattggg ccctaaagtt aataagggac
     9001 ctcgaggggg ggcccggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt
     9061 ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     9121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     9181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga
     9241 tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
     9301 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     9361 aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca
     9421 aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca
     9481 gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct
     9541 gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga
     9601 ctagaaccgg agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc
     9661 ccgcgtcagc accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg
     9721 ctgcaccaag ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc
     9781 caggatgctt gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct
     9841 ggcccgcagc acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg
     9901 cctgcgtagc ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt
     9961 gaccgtgttc gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag
    10021 cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc
    10081 ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc
    10141 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
    10201 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
    10261 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
    10321 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
    10381 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
    10441 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
    10501 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
    10561 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
    10621 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
    10681 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
    10741 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
    10801 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
    10861 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
    10921 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
    10981 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
    11041 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
    11101 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
    11161 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
    11221 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
    11281 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
    11341 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
    11401 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
    11461 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
    11521 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
    11581 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg
    11641 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
    11701 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
    11761 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
    11821 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
    11881 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
    11941 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
    12001 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
    12061 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
    12121 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
    12181 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
    12241 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
    12301 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
    12361 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
    12421 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
    12481 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
    12541 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
    12601 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
    12661 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
    12721 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
    12781 aaaaggtcga aaaggtgtct ttcctgtgga tagcacgtac attgggaacc caaagccgta
    12841 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
    12901 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
    12961 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
    13021 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
    13081 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
    13141 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
    13201 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    13261 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    13321 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
    13381 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
    13441 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
    13501 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    13561 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    13621 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    13681 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    13741 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    13801 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    13861 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    13921 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    13981 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    14041 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    14101 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    14161 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    14221 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    14281 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
    14341 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
    14401 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
    14461 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
    14521 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
    14581 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
    14641 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
    14701 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
    14761 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
    14821 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
    14881 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
    14941 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
    15001 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
    15061 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
    15121 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
    15181 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
    15241 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
    15301 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
    15361 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
    15421 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
    15481 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
    15541 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
    15601 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
    15661 ggggatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    15721 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
    15781 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    15841 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    15901 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    15961 agctatgaca tgattacg
//
