LOCUS       sequence-47612-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCJF604 - pDESTR4-R3(ttTi4391, I)
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(99..105,106..123,124..129))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(154..277)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(285..302)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(285..301)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(451..462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
       61 tcctgaaaat ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata
      121 aagtgtaaag cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta
      181 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
      241 ttttacgttt ctcgttcaac tttattatac atagttgata attcactggc cgtcgtttta
      301 cactttgagc caatttatcc aagtccttgt aaaaagtatt cgaaaattgt taacggataa
      361 aatgtttatt ataatatcaa aaacaatttg tcagttgacc actttttgat atatttgaca
      421 gaaaccggga tgaattggct caaagttagg gcgcccttct attacagggt ttctgataac
      481 aaaccggtta ttaactccca acaagggatg atttcaattc atcatgctca aattgaccca
      541 aattaagtta catgacaaat tcatcgccct tttcaactct tcttggctca tcatctgtta
      601 ttctgttcct attctgtctg caccctatac ccctttgcat actctctcgt cattcctttc
      661 ttggatagtg tcttcttctc cccagctctg ctacttctat gacttgcgcc cgctgctttt
      721 ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct tttctccttc ctttttccct
      781 tctcaatctt cctctttttc ccatttctgt caatcattcg aagaagaaga agaagaagac
      841 cctcattcat
//
