LOCUS       sequence-476121-        2438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rps14-rps7 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_018_rps14-rps7_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      382..439
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(463..480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(634..1222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(714..733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1405..1499)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1520..2179)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2094..2113
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2341..2359
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2363..2406)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2409..2430
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac ttttcgtttt gatatcgtaa acttagttat atgcatatct attaagtagc
       61 gattttcaaa gaggcagttg gcaggacgtc cccttacggg aatataaata ttagtggcag
      121 tggtaccgcc actgcctata tttatatact ccgaaggaac ttgttagccg ataggcgagg
      181 caacaaattt atttattgta tataaatatc cactaaaatt tatttgcccg aaggggacgt
      241 cctattaaac catcacataa ctaaaattgc ttatttggta tgaaagtttg catctatttt
      301 aaccatttag taaaaataat gatgcttttt taaaataaaa aatgtgagac cactcatcgc
      361 gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
      421 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      481 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      541 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      601 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      661 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      721 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      781 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      841 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      901 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      961 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1021 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1081 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1141 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1201 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1261 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1321 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1381 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1441 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1501 aagcgagctc gatatcaaat tacgccccgc cctgccactc gtcgcagtac tgttgtaatt
     1561 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1621 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1681 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1741 ctgatacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1801 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1861 tccagagtga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1921 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1981 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     2041 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     2101 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2161 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     2221 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     2281 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2341 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2401 gatttctgga attcgcggcc gcttctagag cgatgagt
//
