LOCUS       sequence-476124-        2648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  atpA promoter + 5'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2-3_003_atpA_Promoter+5'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      592..649
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(673..690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(844..1432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(924..943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1615..1709)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1730..2389)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2304..2323
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2551..2569
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2573..2616)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2619..2640
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggga gcaaaagtca tttttataac tagtctcaaa atacctataa acccattgtt
       61 cttctctttt agctctaaga acaatcaatt tataaatata tttattatta tgctataata
      121 taaatactat ataaatacat ttaccttttt ataaatacat ttaccttttt tttaatttgc
      181 atgattttaa tgcttatgct atctttttta tttagtccat aaaaccttta aaggaccttt
      241 tcttatggga tatttatatt ttcctaacaa agcaatcggc gtcataaact ttagttgctt
      301 acgacgcctg tggacgtccc ccccttcccc ttacgggcaa gtaaacttag ggattttaat
      361 gcaataaata aatttgtcct cttcgggcaa atgaatttta gtatttaaat atgacaaggg
      421 tgaaccatta cttttgttaa caagtgatct taccactcac tatttttgtt gaattttaaa
      481 cttatttaaa attctcgaga aagattttaa aaataaactt ttttaatctt ttatttattt
      541 tttctttttt aatgtgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa
      601 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
      661 tatgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      721 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      781 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      841 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1021 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1141 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1201 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1261 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1321 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1381 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     1441 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     1501 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     1561 taaatcaatc taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc
     1621 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     1681 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     1741 cctgccactc gtcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1801 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1861 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1921 caaaactggt gaaactcacc cagggattgg ctgatacgaa aaacatattc tcaataaacc
     1981 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     2041 gaaactgccg gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct
     2101 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     2161 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     2221 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     2281 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2341 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2401 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     2461 ggtgaaagtt ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa
     2521 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     2581 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag
     2641 cgatgagt
//
