LOCUS       sequence-476128-        2214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MYC C-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5a_006_MYC-tag
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..43
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             50..79
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             86..115
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      158..215
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(239..256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(410..998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(490..509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1181..1275)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1296..1955)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1870..1889
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2117..2135
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2139..2182)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2185..2206
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct ttagaacaaa aacttatttc agaggaagat ttaaacggtg agcaaaaatt
       61 aatttctgag gaagacttaa atggtgaaca aaaactgatt tcggaggaag atctggggta
      121 tgagaccact catcgcgact agtagcggcc gctgcagtcc ggcaaaaaag ggcaaggtgt
      181 caccaccctg ccctttttct ttaaaaccga aaagattact tcgcgttatg caggcttcct
      241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc
      421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      541 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
      601 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      661 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      721 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      781 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      841 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      901 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      961 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1021 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1081 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1141 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg
     1201 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
     1261 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcgtcg
     1321 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     1381 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1441 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1501 ctcacccagg gattggctga tacgaaaaac atattctcaa taaacccttt agggaaatag
     1561 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1621 tcgtcgtggt attcactcca gagtgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1681 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     1741 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1801 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     1861 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     1921 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     1981 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     2041 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     2101 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     2161 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagagcgat gagt
//
