LOCUS       sequence-47613-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCJF604 - pDESTR4-R3(ttTi4391, I)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     586..602
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     586..602
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    622..745
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggtctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gatacctgat
      181 caccgttttc aacaagatta cagacatcgc agtgaaatcg aggaggagtg tatcaagcag
      241 ctattgatga ttcctccgat gtatgcggat tttgactatt attattgaga ataaagcttg
      301 ttataactcc tacagtaacc ccacagtacc tcgacagtat ctctacagtt cctctacaat
      361 aatcttacag tacccctaca gtacctcaca ataccactta ggtacctcta cagtacctct
      421 acagtatcct tacagtcccc ctacagcacc cctacagtac ttctacagta ctcccatggt
      481 atccttacag tacccctaaa caataatctt gcagtaccgc tacagtgccc cttcggtttc
      541 tctacggtat agatgcaaca cccctactca gtaaaaccgt tcatacagga aacagctatg
      601 accatgatta cgccaagcta tcaactttgt atagaaaagt tgaacgagaa acgtaaaatg
      661 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
      721 aaacacaaca tatccagtca ctatggtcga cctgcagact ggctgtgtat aagggagcct
      781 gacatttata ttccccagaa catcaggtta atggcgtttt tgat
//
