LOCUS       sequence-476131-        2775 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCerulean3 reporter gene, codon optimized for Chlamydomonas 
            reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_010_mCerulean3
FEATURES             Location/Qualifiers
     source          1..2775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      742..799
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(823..840)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(994..1582)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1074..1093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1765..1859)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1880..2539)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2454..2473
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2701..2719
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2723..2766)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gtctcaaatg gtttctaaag gtgaagaatt attcactggt gttgtaccaa ttttagttga
       61 attagatggt gatgttaacg gtcacaaatt ctctgtttca ggtgaaggtg aaggtgatgc
      121 tacatacggt aaattaacat taaaattcat ttgtactaca ggtaaattac cagtaccatg
      181 gccaacatta gttacaactt taacttgggg tgtacaatgt ttcgctcgtt acccagatca
      241 catgaaacaa cacgattttt tcaaatcagc tatgcctgaa ggttacgtac aagaacgtac
      301 tatttttttc aaagacgatg gtaactacaa aacacgtgct gaagttaaat tcgaaggtga
      361 tacattagta aaccgtattg aattaaaagg tattgacttc aaagaagatg gtaacatttt
      421 aggtcacaaa ttagaataca acgctatttc tgataacgtt tacattacag ctgacaaaca
      481 aaaaaacggt attaaagcta acttcaaaat tcgtcacaac attgaagatg gttcagttca
      541 attagctgat cactaccaac aaaacactcc aattggtgat ggtccagtat tattacctga
      601 taaccactac ttatctactc aatcaaaatt atctaaagat ccaaacgaaa aacgtgatca
      661 catggtatta ttagaattcg taactgctgc tggtattact ttaggtatgg atgaattata
      721 caaagcttcg agaccctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
      781 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      841 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      901 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      961 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
     1021 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1081 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1141 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1201 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1261 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1321 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1381 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1441 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1501 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1741 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1801 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1861 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1921 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1981 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     2041 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     2101 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     2161 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     2221 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     2281 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     2341 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     2401 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     2461 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     2521 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     2581 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     2641 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2701 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2761 gatttctgga attcg
//
