LOCUS       sequence-476132-        3147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phosphite oxidoreductase selection gene, codon optimized for 
            Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_023_ptxD-marker
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1023..1049
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      1091..1148
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1172..1189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1343..1931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1423..1442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2114..2208)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2229..2888)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2803..2822
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3050..3068
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3072..3115)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3118..3139
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaat gttaccaaaa ttagtaatta ctcaccgtgt tcacgacgaa attttacaat
       61 tattagctcc acactgtgaa ttaatgacaa accaaactga ttctacttta acacgtgaag
      121 aaattttacg tcgttgtcgt gacgcacaag ctatgatggc tttcatgcca gatcgtgttg
      181 atgctgattt cttacaagct tgtccagaat tacgtgtagt tggttgtgct ttaaaaggtt
      241 tcgataactt cgatgttgat gcttgtactg ctcgtggtgt atggttaaca ttcgtaccag
      301 atttattaac agtaccaaca gctgaattag ctattggttt agctgtaggt ttaggtcgtc
      361 acttacgtgc tgctgatgct ttcgtacgtt caggtgaatt ccaaggttgg caaccacaat
      421 tctatggtac tggtttagat aacgctactg taggtatttt aggtatgggt gctattggtt
      481 tagctatggc tgaccgttta caaggttggg gtgctacttt acaatatcac gctcgtaaag
      541 ctttagatac tcaaacagaa caacgtttag gtttacgtca agtagcttgt tcagaattat
      601 ttgcttcttc tgatttcatt ttattagctt taccattaaa cgctgatact caacacttag
      661 taaacgctga attattagct ttagttcgtc caggtgcttt attagttaac ccatgtcgtg
      721 gttcagtagt agatgaagct gctgtattag ctgctttaga acgtggtcaa ttaggtggtt
      781 acgctgctga tgtttttgaa atggaagatt gggctcgtgc tgaccgtcca cgtttaattg
      841 accctgcttt attagctcac ccaaacactt tatttacacc acacattggt tcagctgttc
      901 gtgctgttcg tttagaaatt gaacgttgtg ctgctcaaaa cattattcaa gtattagctg
      961 gtgctcgtcc aattaacgct gctaaccgtt taccaaaagc tgaacctgct gcttgtggtt
     1021 cttacccata cgatgttcca gattacgctg ctttgagacc actcatcgcg actagtagcg
     1081 gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac
     1141 cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg cgctcggtcg
     1201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1321 aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa
     1381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1741 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2101 gctcgaggct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     2161 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     2221 atatcaaatt acgccccgcc ctgccactcg tcgcagtact gttgtaattc attaagcatt
     2281 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     2341 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     2401 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgatacgaaa
     2461 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2521 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagtgat
     2581 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2641 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     2701 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2761 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2821 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2881 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2941 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat caactcgagt
     3001 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     3061 cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa
     3121 ttcgcggccg cttctagagc gatgagt
//
