LOCUS       sequence-476135-        2979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aminoglycoside-3''-9-adenylyltransferase selection gene, codon 
            optimized for Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_034_aadA
FEATURES             Location/Qualifiers
     source          1..2979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      923..980
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1004..1021)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1175..1763)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1255..1274)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1946..2040)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2061..2720)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2635..2654
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2882..2900
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2904..2947)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2950..2971
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaat ggcaatgcgt actccagaag aacttagtaa tcttattaaa gatttaattg
       61 aacaatacac tccagaagtg aaaatgtcca tggctcgtga agcggttatc gccgaagtat
      121 caactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
      181 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt
      241 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
      301 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
      361 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
      421 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
      481 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
      541 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
      601 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
      661 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
      721 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
      781 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
      841 tccactacgt gaaaggcgag atcactaagg tagttggcaa agctttgaga ccactcatcg
      901 cgactagtag cggccgctgc agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt
      961 tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc
     1021 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1081 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1141 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1201 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1261 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1321 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1381 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1441 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1501 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1561 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     1621 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1681 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     1741 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     1801 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     1861 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     1921 cttggtctga cagctcgagg cttggattct caccaataaa aaacgcccgg cggcaaccga
     1981 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     2041 caagcgagct cgatatcaaa ttacgccccg ccctgccact cgtcgcagta ctgttgtaat
     2101 tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc
     2161 agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg
     2221 aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg
     2281 gctgatacga aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg
     2341 taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca
     2401 ctccagagtg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca
     2461 ctatcccata tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc
     2521 atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg
     2581 gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact
     2641 gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat
     2701 ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa
     2761 aatacgcccg gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg
     2821 atcaactcga gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     2881 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
     2941 tgatttctgg aattcgcggc cgcttctaga gcgatgagt
//
