LOCUS       sequence-476137-        2518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rbcL 3'UTR + rbcL CDS Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_009_rbcL_3'UTR+rbcL_CDS
FEATURES             Location/Qualifiers
     source          1..2518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      462..519
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(543..560)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(714..1302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(794..813)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1485..1579)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1600..2259)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2174..2193
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2421..2439
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2443..2486)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2489..2510
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct taagtactca agctcgtaac gaaggtcgtg accttgctcg tgaaggtggc
       61 gacgtaattc gttcagcttg taaatgatct ccagaacttg ctgctgcatg tgaagtttgg
      121 aaagaaatta aattcgaatt tgatactatt gacaaacttt aatttttatt tttcatgatg
      181 tttatgtgaa tagcataaac atcgttttta tttttatggt gtttaggtta aatacctaaa
      241 catcatttta catttttaaa attaagttct aaagttatct tttgtttaaa tttgcctgtc
      301 tttataaatt acgatgtgcc agaaaaataa aatcttagct ttttattata gaatttatct
      361 ttatgtatta tattttataa gttataataa aagaaatagt aacatactaa agcggatgta
      421 cgcttgagac cactcatcgc gactagtagc ggccgctgca gtccggcaaa aaagggcaag
      481 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
      541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
      721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1261 gtttgcaagc agcagattac gcgcagaaga aaaggatctc aagaagatcc tttgatcttt
     1321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1441 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
     1501 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1561 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1621 gtcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1681 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1741 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1801 gaaactcacc cagggattgg ctgatacgaa aaacatattc tcaataaacc ctttagggaa
     1861 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1921 gaaatcgtcg tggtattcac tccagagtga tgaaaacgtt tcagtttgct catggaaaac
     1981 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     2041 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     2101 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     2161 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     2221 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     2281 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     2341 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     2401 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2461 ccttagcttt cgctaaggat gatttctgga attcgcggcc gcttctagag cgatgagt
//
