LOCUS       sequence-476138-        2760 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  petA 3'UTR Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_015_petA_3'UTR_Cr
FEATURES             Location/Qualifiers
     source          1..2760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      704..761
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(785..802)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(956..1544)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1036..1055)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1727..1821)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1842..2501)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2416..2435
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2663..2681
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2685..2728)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2731..2752
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct taatatttaa ttttttgtag ggctgctgtg cagctcctac aaattttagt
       61 atgttatttt taaagtttga tatactgaaa acaaagttct acttgaacga tatttagctt
      121 ttaatgctat aatatagcgg actaagccgt tggcaattta gctgccaatt aattttattc
      181 gaaggatgta aacctgctaa cgatatttat atataagcat tttaatactc cgagggaggc
      241 ctctaacctt tagcaagtaa gtaaacttcc ccttcggggc agcaaggcag cagatttaaa
      301 ttctccaaag gaggcagttg atatcagtaa accccttcga tgactctggc attgatgcaa
      361 agcatgggga aactaaagtt cctccactgc ctccttcccc ttccctttcg ggacgtcccc
      421 ttccccttac gggcaagtaa acttagggat tttaatgcaa taaataaatt tgtcccctta
      481 cgggacgtca gtggcagttg cgaagtatta atattgtata taaatataga atgtttacat
      541 actccgaagg aggacgtcag tggcagtggt accgccactg cctgcttcgc agtatataaa
      601 tatcctattt taatactccg aaggaggcag tggtggtccc actgccacta aaatttattt
      661 gccgcttgag accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca
      721 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
      781 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      841 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      901 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      961 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1021 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1081 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1141 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1201 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1261 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1321 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1381 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1441 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1501 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1561 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1621 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     1681 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
     1741 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     1801 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     1861 tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     1921 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     1981 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     2041 gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg
     2101 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     2161 cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa
     2221 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     2281 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     2341 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     2401 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     2461 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     2521 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     2581 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
     2641 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     2701 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt
//
