LOCUS       sequence-476143-        4757 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for constitutive expression of NRF1 alternative exon-7 
            minigene .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4757)
  TITLE     A Scalable Exon Deletion Platform Coupled To Single-Cell 
            Transcriptomics Reveals Exons Shaping Gene Expression Dynamics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET01-NRF1-Ex7-minigene
FEATURES             Location/Qualifiers
     source          1..4757
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(107..126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1285..1304
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        complement(1859..2055)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1873..2008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2163..2182
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4455..4473)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4541..4645
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4646..4714,4715..4757,1..749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggctgctgct tgcaaacaaa
      841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      901 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
      961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1081 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1141 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1201 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1261 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1321 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1381 gaaaaacgcc agcaacggag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     1441 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     1501 caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt
     1561 ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc
     1621 tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat
     1681 cagcggtcca atgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc
     1741 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     1801 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg gggagctttt
     1861 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     1921 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     1981 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     2041 actaattgag atgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     2101 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     2161 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     2221 acgatgcgtc cggcgtagag gatctcagga tatagtagtt tcgcttttgc atagggaggg
     2281 ggaaatgtag tcttatgcaa tactcttgta gtcttgcaac atgcttatgt aacgatgagt
     2341 tagcaacatg ccttataagg agagaaaaag caccgtgcat gccgattggt gggagtaagg
     2401 tggtatgatc gtggtatgat cgtgccttgt taggaaggca acagacgggt ctaacacgga
     2461 ttggacgaac cactgaattc cgcattgcag agatattgta tttaagtgcc tagctcgata
     2521 caataaacgc catttgacca ttcaccacat tggtgtgcac ctcaagcttc ctgcatgctg
     2581 ctgctgctgc tgctgctggg cctgaggcta cagctctccc tgggcatcat cccagttgag
     2641 gaggagaacc cggacttctg gaaccgcgag gcagccgagg ccctgggtgc cgccaagaag
     2701 ctgcagcctg cacagacagc cgccaagaac ctcatcatct tcctgggcga tgggatgggg
     2761 gtgtctacgg tgacagctgc caggatcgat ccgcttcctg cccctgctgg ccctgctcat
     2821 cctctgggag ccccgccctg cccaggcttt tgtcaaacag cacctttgtg gttctcactt
     2881 ggtggaagct ctctacctgg tgtgtgggga gcgtggattc ttctacacac ccatgtcccg
     2941 ccgcgaagtg gaggacccac aaggtaagct ctgctcctga attaattcta tcccaagtgc
     3001 taactaccct gtttgtcttt cacccttgag accttgtaaa ttgtgcccta ggtgtggagg
     3061 gtctcaggct aaccagtggg gggcacattt ctgtgggcag ctagacatat gtaaacatgg
     3121 tagctgccag gaaggagtga gaatccttcc ttaagtctcc taggtggtga cgggtggcta
     3181 ggccccagga taggtaccgg gccggttgga atagggtgta aactagactt tgatcaagaa
     3241 gaaaccaatg agaagccctc tttgatactt cgtacacagc ttctttctac cagaaccagt
     3301 ctgtgggagg taatcctagg agagtctaat aagaggtttg gtttggtttg atttgataaa
     3361 gaagtagctg cttattgcat attgggacta aataaccaac gttctttgtg gcttaatgtg
     3421 cttttccggt catttggtga caggtttcat ggacccaggc actacggacc atagttaaaa
     3481 actgttataa acagcatggg cgggaagacc ttttgtatgc ctttgaagat cagcaaacgc
     3541 aaacacaggc cacagccaca catagtatag ctcatcttgt accatcacag actgtagtcc
     3601 agacttttag taaccctgat ggcactgtct cacttatcca ggtgagtaaa cctgagggct
     3661 accagactgt ggcttctgag atggatcagt gggcacctca cctcagacta gggaaagttt
     3721 cctttgtgcg aactcttgta tgaactttat atagctcccc aagatcttcc tacctgaaat
     3781 tgatttctga agaatagttt aaattctttg catttgctaa atacagagct atgagtagct
     3841 tcctggacag gccgccaccg cggtggagct cggtacctat ttggggaccc catagagcac
     3901 tgcactgact gagggatggt aacaggatgt gtaggttttg gaggcccata tgtccattca
     3961 tgaccagtga cttgtctcac agccatgcaa cccttgcctc ctgtgctgac ttagcagggg
     4021 ataaagtgag agaaagcctg ggctaatcag ggggtcgctc agctcctcct aactggattg
     4081 tcctatgtgt ctttgcttct gtgctgctga tgctctgccc tgtgctgaca tgacctccct
     4141 ggcagtggca caactggagc tgggtggagg cccgtgacct tcagaccttg gcactggagg
     4201 tggcccggca gaagcgcggc atcgtggatc agtgctgcac cagcatctgc tctctctacc
     4261 aactggagaa ctactgcaac taggcccacc actaccctgt ccacccctct gcaatgaata
     4321 aaacctttga aagagcacta caagttgtgt gtacatgcgt gcatgtgcat atgtggtgcg
     4381 gggggaacat gagtggggct ggctggagtg gcgatgataa gctgtcaaac atgagaattc
     4441 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     4501 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4561 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4621 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4681 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4741 agatgctgaa gatcagt
//
