LOCUS       sequence-476145-        2208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psaC 3'UTR Nicotiana tabacum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_048_psaC_3'UTR_Nt
FEATURES             Location/Qualifiers
     source          1..2208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      152..209
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(233..250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(404..992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(484..503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1175..1269)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1290..1949)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1864..1883
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2111..2129
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2133..2176)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2179..2200
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct taatgatacg ttcgagaaaa ctctacttga atccatttaa ttttttttac
       61 cgacaaacct gtgctcgaaa atcacaatat tttgagcacg ggtttttatg cgcttgagac
      121 cactcatcgc gactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac
      181 cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca
      241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
      301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
      361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc
      421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
      481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
      541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
      601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
      661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
      781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
      841 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
      901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
      961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1141 atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc
     1201 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1261 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc gtcgcagtac
     1321 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1381 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1441 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1501 cagggattgg ctgatacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1561 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1621 tggtattcac tccagagtga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1681 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1741 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1801 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1861 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     1921 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     1981 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     2041 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
     2101 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     2161 cgctaaggat gatttctgga attcgcggcc gcttctagag cgatgagt
//
