LOCUS       sequence-476147-        8955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfection-based all-in-one base editor plasmid that expresses 
            both adenine base editor (ABE8e-nSaCas9) and single guide RNAs 
            (sgRNA) with BpiI cloning sites to clone sgRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8955)
  TITLE     A Scalable Exon Deletion Platform Coupled To Single-Cell 
            Transcriptomics Reveals Exons Shaping Gene Expression Dynamics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pX462-ABE8e-nSaCas9
FEATURES             Location/Qualifiers
     source          1..8955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             16..69
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             70..669
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(70..89)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     566..586
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(697..714)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    703..910
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   919..1059
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   919..1048
                     /label=AAV2 ITR
     rep_origin      1134..1589
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1221..1240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1431..1452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1606..1625)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1725..1747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1785..1803)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1871..1975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1976..2044,2045..2836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2194..2213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3007..3595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3496..3515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3657..3905
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3657..3677
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3828..3847
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3924..3999
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     enhancer        4096..4381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        4383..4660
                     /label=chicken beta-actin promoter
     intron          4661..4888
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      4900..4909
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4909..4974
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8749..8775
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8785..8811
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8821..8847
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8857..8883
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8893..8919
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             8929..8955
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
ORIGIN
        1 gaattcggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
       61 cctggcccaa tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
      121 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
      181 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
      241 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
      301 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
      361 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
      421 cccaaggagc ccgcgtggtt cctggccacc gtcggagtct cgcccgacca ccagggcaag
      481 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
      541 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
      601 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
      661 ggtgcctgag aattctaact agagctcgct gatcagcctc gactgtgcct tctagttgcc
      721 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
      781 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
      841 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc
      901 atgctgggga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
      961 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     1021 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     1081 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     1141 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     1201 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     1261 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     1321 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     1381 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     1441 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     1501 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1561 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     1621 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     1681 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     1741 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     1801 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     1861 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     1921 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1981 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2041 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2101 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2161 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2221 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2281 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2341 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2401 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2461 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2521 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2581 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2641 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg
     2701 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2761 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     2821 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     2881 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     2941 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3001 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3061 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     3181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3241 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3301 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3361 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     3421 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3481 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3541 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3601 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtgagg
     3661 gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt agagagataa
     3721 ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt
     3781 aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc
     3841 ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa
     3901 acaccgggtc ttcgagaaga cctgttatag tactctggaa acagaatcta ctataacaag
     3961 gcaaaatgcc gtgtttatct cgtcaacttg ttggcgagat tttttgtttt agagctagaa
     4021 atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat
     4081 ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4141 cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg
     4201 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     4261 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat
     4321 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     4381 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     4441 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     4501 ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga ggcggagagg
     4561 tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg cgaggcggcg
     4621 gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg cgcgctgcct
     4681 tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct gactgaccgc
     4741 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagctgag
     4801 caagaggtaa gggtttaagg gatggttggt tggtggggta ttaatgttta attacctgga
     4861 gcacctgcct gaaatcactt tttttcaggt tggaccggtg ccaccatgga ctataaggac
     4921 cacgacggag actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc
     4981 tctgaggtgg agttttccca cgagtactgg atgagacatg ccctgaccct ggccaagagg
     5041 gcacgggatg agagggaggt gcctgtggga gccgtgctgg tgctgaacaa tagagtgatc
     5101 ggcgagggct ggaacagagc catcggcctg cacgacccaa cagcccatgc cgaaattatg
     5161 gccctgagac agggcggcct ggtcatgcag aactacagac tgattgacgc caccctgtac
     5221 gtgacattcg agccttgcgt gatgtgcgcc ggcgccatga tccactctag gatcggccgc
     5281 gtggtgtttg gcgtgaggaa ctcaaaaaga ggcgccgcag gctccctgat gaacgtgctg
     5341 aactaccccg gcatgaatca ccgcgtcgaa attaccgagg gaatcctggc agatgaatgt
     5401 gccgccctgc tgtgcgattt ctatcggatg cctagacagg tgttcaatgc tcagaagaag
     5461 gcccagagct ccatcaactc cggaggatct agcggaggat cctctggcag cgagacacca
     5521 ggaacaagcg agtcagcaac accagagagc agtggcggca gcagcggggg gtcagctagc
     5581 gctaagcgaa attacattct ggggctggcc attggcatta catcagtggg ctatggcatc
     5641 attgactacg agacaaggga cgtgatcgac gccggcgtga gactgttcaa ggaggccaac
     5701 gtggagaaca atgagggccg gagatccaag aggggagcaa ggcgcctgaa gcggagaagg
     5761 cgccacagaa tccagagagt gaagaagctg ctgttcgatt acaacctgct gaccgaccac
     5821 tccgagctgt ctggcatcaa tccttatgag gccagagtga agggcctgtc ccagaagctg
     5881 tctgaggagg agtttagcgc cgccctgctg cacctggcaa agaggagagg cgtgcacaac
     5941 gtgaatgagg tggaggagga caccggcaac gagctgtcca caaaggagca gatcagccgc
     6001 aattccaagg ccctggagga gaagtatgtg gccgagctgc agctggagcg gctgaagaag
     6061 gatggcgagg tgaggggctc catcaatcgc ttcaagacct ctgactacgt gaaggaggcc
     6121 aagcagctgc tgaaggtgca gaaggcctac caccagctgg atcagtcctt tatcgataca
     6181 tatatcgacc tgctggagac aaggcgcaca tactatgagg gaccaggaga gggctctccc
     6241 ttcggctgga aggacatcaa ggagtggtac gagatgctga tgggccactg cacctatttt
     6301 ccagaggagc tgagaagcgt gaagtacgcc tataacgccg atctgtacaa cgccctgaat
     6361 gacctgaaca acctggtcat caccagggat gagaacgaga agctggagta ctatgagaag
     6421 ttccagatca tcgagaacgt gttcaagcag aagaagaagc ctacactgaa gcagatcgcc
     6481 aaggagatcc tggtgaacga ggaggacatc aagggctacc gcgtgacctc cacaggcaag
     6541 ccagagttca ccaatctgaa ggtgtatcac gatatcaagg acatcacagc ccggaaggag
     6601 atcatcgaga acgccgagct gctggatcag atcgccaaga tcctgaccat ctatcagagc
     6661 tccgaggaca tccaggagga gctgaccaac ctgaatagcg agctgacaca ggaggagatc
     6721 gagcagatca gcaatctgaa gggctacacc ggcacacaca acctgagcct gaaggccatc
     6781 aatctgatcc tggatgagct gtggcacaca aacgacaatc agatcgccat ctttaaccgg
     6841 ctgaagctgg tgccaaagaa ggtggacctg tcccagcaga aggagatccc aaccacactg
     6901 gtggacgatt tcatcctgtc tcccgtggtg aagcggagct tcatccagag catcaaagtg
     6961 atcaacgcca tcatcaagaa gtacggcctg cccaatgata tcatcatcga gctggccagg
     7021 gagaagaact ccaaggacgc ccagaagatg atcaatgaga tgcagaagag gaaccgccag
     7081 accaatgagc ggatcgagga gatcatcaga accacaggca aggagaacgc caagtacctg
     7141 atcgagaaga tcaagctgca cgatatgcag gagggcaagt gtctgtattc tctggaggcc
     7201 atccctctgg aggacctgct gaacaatcca ttcaactacg aggtggatca catcatcccc
     7261 cggagcgtga gcttcgacaa ttcttttaac aataaggtgc tggtgaagca ggaggagaac
     7321 agcaagaagg gcaataggac ccctttccag tacctgtcta gctccgattc taagatcagc
     7381 tacgagacat tcaagaagca catcctgaat ctggccaagg gcaagggccg catcagcaag
     7441 accaagaagg agtacctgct ggaggagcgg gacatcaaca gattctccgt gcagaaggac
     7501 ttcatcaacc ggaatctggt ggacaccaga tacgccacac gcggcctgat gaatctgctg
     7561 cggtcttatt tcagagtgaa caatctggat gtgaaggtga agagcatcaa cggcggcttc
     7621 acctcctttc tgcggagaaa gtggaagttt aagaaggagc gcaacaaggg ctataagcac
     7681 cacgccgagg atgccctgat catcgccaat gccgacttca tctttaagga gtggaagaag
     7741 ctggacaagg ccaagaaagt gatggagaac cagatgttcg aggagaagca ggccgagagc
     7801 atgcccgaga tcgagacaga gcaggagtac aaggagattt tcatcacacc tcaccagatc
     7861 aagcacatca aggacttcaa ggactacaag tattctcaca gggtggataa gaagcccaac
     7921 cgcaagctga tcaatgacac cctgtatagc acacggaagg acgataaggg caataccctg
     7981 atcgtgaaca atctgaacgg cctgtacgac aaggataatg acaagctgaa gaagctgatc
     8041 aacaagtctc ccgagaagct gctgatgtac caccacgatc ctcagacata tcagaagctg
     8101 aagctgatca tggagcagta cggcgacgag aagaacccac tgtataagta ctatgaggag
     8161 acaggcaact acctgacaaa gtatagcaag aaggataatg gccccgtgat caagaagatc
     8221 aagtactatg gcaacaagct gaatgcccac ctggacatca ccgacgatta ccctaactct
     8281 cgcaataagg tggtgaagct gagcctgaag ccataccggt tcgacgtgta cctggacaac
     8341 ggcgtgtata agtttgtgac agtgaagaat ctggatgtga tcaagaagga gaactactat
     8401 gaggtgaaca gcaagtgcta cgaggaggcc aagaagctga agaagatcag caaccaggcc
     8461 gagttcatcg cctcttttta caagaatgac ctgatcaaga tcaatggcga gctgtataga
     8521 gtgatcggcg tgaacaatga tctgctgaac agaatcgaag tgaatatgat cgacatcacc
     8581 tacagggagt atctggagaa catgaatgat aagaggcccc ctcatatcat caagaccatc
     8641 gcctctaaga cacagagcat caagaagtac agcacagaca tcctggggaa cctgtatgaa
     8701 gtcaagagca agaaacatcc tcagattatc aagaaaggca gaattgggcc tgctgctaaa
     8761 cgtgttaagc ttgatggggg tagcccggca gccaagagag tcaaactcga cggcggctcc
     8821 ccagcggcaa aaagggtgaa actagacggg ggtagccccg ccgcgaagcg tgtaaagctg
     8881 gatggaggat cgcctgcggc taagcgagtc aaattagacg gtgggtcacc ggctgcaaag
     8941 cgcgtcaaat tggat
//
