LOCUS       sequence-476148-        3120 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Homology psbA in the M2 position used for genome integration at 
            the psbA locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_007_psbA_3'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..3120
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1064..1121
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1145..1162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1316..1904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1396..1415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2087..2181)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2202..2861)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2776..2795
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3023..3041
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3045..3088)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3091..3112
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgcgc ttccagcgat attaaagtta ttagaagaaa ataaaactag tccatatcca
       61 attccaatta ttccaattga aactatttat gggttattta ctaatatagc tgaagattaa
      121 ttttttttaa aaaaatattt aatgcaattg caataataat aaatagcatt aaatatgtaa
      181 attttaatta actaaaagac ctaatccagc cataatataa acgaaagtga ataaagtgat
      241 atatatctga accgatttga tagcgacgaa aggctaataa cgttgtagca attaaacgtc
      301 tacatatggc gaaaaaaaaa ttggattaac cttcggtaag taaagcgatt aaccttcagt
      361 aagttaagca attaaccttc ggtaagtaaa gcgattaacc ttcagtaagt taagcaatta
      421 accttcggta agtaaagcga ttaaccttcg gtaagtaaag cgattaacct tcggtaagtt
      481 aagcaattaa cggtcgatag actaataaaa atttatgtat ttaaacttgt ctaacagcgt
      541 ataaatataa cagcgtataa atataaatgt aagccaacag gctgtcacga agtgattata
      601 gaacaactag gaagaccaca gcacgtccca aaaaatccac gaaggcattt accttcggta
      661 agataataaa cctcgatgta gagctcataa gagctcaaat ttacatattg taaacgacag
      721 attcacatat agaacaacca ggaagaccgc atcacgtccc ataagatcca cgaaggcatt
      781 taccttcggt aagataataa acctcgatgt agagctcata agagctcaaa tttacatatt
      841 gtaaacgaca gattcacata tagaacaacc aggaagacca catcacgtcc cataagatcc
      901 acgaaggcat ttaccttcgg taagataata aacctcgatg tagaggccat aagagctcaa
      961 atttacatat cgtagacgac agatttacat atcgtagacg acaaatactt tagacggaga
     1021 cgagactgag accactcatc gcgactagta gcggccgctg cagtccggca aaaaagggca
     1081 aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg
     1141 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1201 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1261 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1321 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1381 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1441 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1501 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1561 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1621 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1681 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1741 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1801 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1861 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1921 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1981 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2041 tctaaagtat atatgagtaa acttggtctg acagctcgag gcttggattc tcaccaataa
     2101 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     2161 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     2221 tcgtcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacaaacg
     2281 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     2341 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     2401 gtgaaactca cccagggatt ggctgatacg aaaaacatat tctcaataaa ccctttaggg
     2461 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     2521 cggaaatcgt cgtggtattc actccagagt gatgaaaacg tttcagtttg ctcatggaaa
     2581 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     2641 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     2701 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     2761 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     2821 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     2881 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     2941 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
     3001 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
     3061 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt
//
