LOCUS       sequence-476149-        3486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5'Homology psbA reverse in the M2 position used for genome 
            integration at the psbA locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_020_psbA_5'Hom_rev_Cr
FEATURES             Location/Qualifiers
     source          1..3486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1430..1487
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1511..1528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1682..2270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1762..1781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2453..2547)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2568..3227)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3142..3161
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3389..3407
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3411..3454)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3457..3478
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgcgc tcgtatccat gctagcaata tctgatggta cttgcatttc ataagtttgg
       61 cctggaataa ccaccgtttc ggaagtacct gtcgctttaa gttttatagc taaatctaaa
      121 gtttctttaa gtcttttagc tgtattaaat actccacgac tttcccttac gggacaataa
      181 ataaatttgt ccccttcccc ttacgggacg tcagtggcag ttgcctgcca actgcctcct
      241 tcggagtatt aaaatcctat atttatatac tcctaagttt acttgcccaa tatttatatt
      301 aggcagttgg caggcaactg ccactgacgt cccgaagggg aaggggaagg acgtcccctt
      361 cgggtaaata aattttagtg gcagtggtac caccactgcc tgcttcctcc ttccccttcg
      421 ggcaagtaaa cttagaataa aatttatttg ctgcgctagc aggtttacat actcctaagt
      481 ttacttgccc gaaggggaag gaggacgtcc ccttacggga atataaatat tagtggcagt
      541 ggtacaataa ataaattgta tgtaaacccc ttcgggcaac taaagtttat cgcagtatat
      601 aaatatagaa tgtttacata ctccgaagga ggacgccagt ggcagtggta ccgccactgc
      661 ctgctccgca gtattaacat cctattttaa tactccgaag gaggcagttg gcaggcaact
      721 gccactaata tttatattcc cgtaagggga cgtcctattt taatactccg aaggaggcag
      781 ttggcaggca actgccacta aaatttattt gcctcctaac ggagcattaa aatcccgaag
      841 gggacgtccc gaaggggaag gggaaggagg caactgcctg cttcctcctt ccccttcggg
      901 caagtaaact tagaataaaa tttatttgct gcgctagcag gtttacatac tcctaagttt
      961 acttgcccga aggggaagga ggacgtcccc ttacgggaat ataaatatta gtggcagtgg
     1021 tacaataaat aaattgtatg taaacccctt cgggcaacta aagtttatcg cagtatataa
     1081 atatcggcag ttggcaggca actgccacta aaattcattt gcccgaaggg gacgtccact
     1141 aatatttata ttcccgtaag gggacgtccc gaaggggaag gggacgtcct aacggagcat
     1201 taaaatccct aagtttactt gcctaggcag ttggcaggat atttatatac gatattaata
     1261 ctttgcaact gccactaaaa tttatttgcc cgaaggggac gtccttcgga gtatataaat
     1321 atcccgtaag gggaagggga tgtcccgtaa ggggaagggg agaagctcca acagaggtgt
     1381 tcgagacgag acagagacca ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa
     1441 agggcaaggt gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta
     1501 tgcaggcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1681 ttttccacag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2101 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2221 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2281 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2341 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2401 aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac
     2461 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     2521 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     2581 tgccactcgt cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2641 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2701 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2761 aaactggtga aactcaccca gggattggct gatacgaaaa acatattctc aataaaccct
     2821 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2881 aactgccgga aatcgtcgtg gtattcactc cagagtgatg aaaacgtttc agtttgctca
     2941 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     3001 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     3061 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     3121 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     3181 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     3241 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     3301 tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac
     3361 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
     3421 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagcg
     3481 atgagt
//
