LOCUS       sequence-47615-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ606 - pDESTR4-R3(ttTi14024, X)
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(88..94,95..112,113..118))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(143..266)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(274..291)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(274..290)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(440..451)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
       61 tcgacggatc ctaactcaaa atccacacat tatacgagcc ggaagcataa agtgtaaagc
      121 ctggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac agtattatgt
      181 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
      241 tcgttcaact ttattataca tagttgataa ttcactggcc gtcgttttac actttgagcc
      301 aatttatcca agtccttgta aaaagtattc gaaaattgtt aacggataaa atgtttatta
      361 taatatcaaa aacaatttgt cagttgacca ctttttgata tatttgacag aaaccgggat
      421 gaattggctc aaagttaggg cgcccttcta ttacagggtt tctgataaca aaccggttat
      481 taactcccaa caagggatga tttcaattca tcatgctcaa attgacccaa attaagttac
      541 atgacaaatt catcgccctt ttcaactctt cttggctcat catctgttat tctgttccta
      601 ttctgtctgc accctatacc cctttgcata ctctctcgtc attcctttct tggatagtgt
      661 cttcttctcc ccagctctgc tacttctatg acttgcgccc gctgcttttc cgctcgttcc
      721 tctcctctct gacgtcttct tctgcttctt ttctccttcc tttttccctt ctcaatcttc
      781 ctctttttcc catttctgtc aatcattcga agaagaagaa gaagaagacc ctcattcat
//
