LOCUS       sequence-476151-       15584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one bimolecular fluorescence complementation (BiFC) vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 15584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTHV3
FEATURES             Location/Qualifiers
     source          1..15584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1153..1863)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1431..1450)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2306..2651)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2681..2855
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(2901..2922)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(2962..3307)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3337..3511
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(3629..4141,4142..4144,4145..4147))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     3822..3841
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4081..4102
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(4222..4898)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8670..8687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8670..8686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8679..8701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8889..8913
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10214..10843
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11272..12345
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12411..12605
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12841..12863)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12949..13089
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13004..13023
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13104..13121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13275..13863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13355..13374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13950..14744)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15169..15193
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    15461..15482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15497..15502,15503..15520,15521..15527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15535..15551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15540..15562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15559..15575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15559..15575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcttacttgt acagctcgtc catgccgccg gtggagtggc ggccctcggc
     1201 gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccaacttgat
     1261 gttgacgttg taggcgccgg gcagctgcac gggcttcttg gccttgtagg tggtcttgac
     1321 ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc tgcttgatct cgcccttcag
     1381 ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc cagcccatgg tcttcttctg
     1441 cattacgggg ccgtcggagg ggaagttggt gccgcgcagc ttcaccttgt agatgaactc
     1501 gccgtcctgc agggaggagt cctgggtcac ggtcaccacg ccgccgtcct cgaagttcat
     1561 cacgcgctcc cacttgaagc cctcggggaa ggacagcttc aagtagtcgg ggatgtcggc
     1621 ggggtgcttc acgtaggcct tggagccgta catgaactga ggggacagga tgtcccaggc
     1681 gaagggcagg gggccaccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg
     1741 gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg
     1801 caccttgaag cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac
     1861 catggatcca gaactcgtaa acttcgtaac cgccttagtc ccttcagaaa cagcatgttt
     1921 cgccaactct ccaggcaaca caagtctcac cgcagtctga atctccctag aagtaatcgt
     1981 cggcttcttg ttgtacctcg caagcttcga agactcacca gcaagtttct caaagatatc
     2041 attgatgaaa ctgttcatga ttcccatggc tttgctggag attccgatat ctggatgaac
     2101 ttgcttcaac accttgaaga tgtagatctt gtatgtctca acgttcttct ttgatctctt
     2161 cttcttcttg tctccggcgc cggctggttc cttcgggagt ttctttccgg cttttggttt
     2221 cttctctgcc gctgctgctg gttcggctgc cggagttttc tctgccggtt tcttctccgc
     2281 tggtttctta tctgccttcg ccatctgtcc tctccaaatg aaatgaactt ccttatatag
     2341 aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
     2401 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     2461 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct
     2521 ttcctttatc gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa
     2581 taaagtgaca gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt
     2641 gaaaagtctc aattgccctt tggtcttctg agactgtatc gatctggatt ttagtactgg
     2701 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     2761 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     2821 tatctgggaa ctactcacac attattatgg agaaattact tgtacagctc gtccatgccg
     2881 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
     2941 gggtccatga gaccggtacc ctgtcctctc caaatgaaat gaacttcctt atatagagga
     3001 agggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
     3061 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
     3121 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttcaacga tggcctttcc
     3181 tttatcgcaa tgatggcatt tgtaggagcc accttccttt tccactatct tcacaataaa
     3241 gtgacagata gctgggcaat ggaatccgag gaggtttccg gatattaccc tttgttgaaa
     3301 agtctcaatt gccctttggt cttctgagac tgtatcgatc tggattttag tactggattt
     3361 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     3421 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     3481 tgggaactac tcacacatta ttatggagaa attagtcttt gctcagggcg gactggtagc
     3541 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     3601 agtggtcggc gagctgcacg ccgccgtcct cgatgttgtg gcggatcttg aagttggcct
     3661 tgatgccgtt cttctgcttg tcggcggtga tatagacgtt gtggctgttg tagttgtact
     3721 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     3781 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     3841 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     3901 cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag cccagggtgg
     3961 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatcagc ttcagggtca
     4021 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4081 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4141 tcaccatggt ctcggatctc attgcccccc ggatctgcga aagctcgaga gagatagatt
     4201 tgtagagaga gactggtgat ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat
     4261 agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat
     4321 atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct
     4381 cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc
     4441 ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt
     4501 gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg
     4561 ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta
     4621 gacgagagtg tcgtgctcca ccatgttcac atcaatccac ttgctttgaa gacgtggttg
     4681 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     4741 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     4801 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     4861 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     4921 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttggcaagct
     4981 gctctagctc acgttgacgg aaactgcaga acgatctcag ttaggtcatg ggaaagctga
     5041 tattcatccc tctgagactc tatataatta aatgcgtttg ggggattagc taattgagaa
     5101 tccatataga gaaatatggc cgcgcagcga cgccggttga agaatatgaa caaggaataa
     5161 gatgaacaag gaagaagatg aacaagcaat aatttaatta attattgtta ataagcataa
     5221 attatgaaaa taattttgat ggtaatgaaa gtattataaa taatatttag gagaaaatat
     5281 tgttaaggaa atatctggtt atgtttattt gttaaggtat atgggttttt atagaaaccc
     5341 aggagcagct atggtaagaa acccaggact caaaaaatgc ctctcaactt ctctcatatg
     5401 ttgcggagtc tggagtccca tttataccat agaaagcatt gggggtatag catggtaatt
     5461 tcacatcgga ctccataccg cggccatcta tataatatta ctgatggccg cgcgaagtcc
     5521 cggtatgcct tttcagccgt tggatctgga gccgtacctt tttcgcgttc ccattttgcc
     5581 cctggagtcc cgagctattt acgaccccct gccaccgagt ccccttcgtg gagcgctctt
     5641 cgtccaacac gtgtcccaac ttttgcgcat ttttgactac ctgtcgcgca cttgaattaa
     5701 agttgccctt ttattttctt ccaattattt tgcggctgag tagtctagtg gatcccccgg
     5761 agctccactt gggataggtt aagaaaatat gtctggcttg taaacgaaaa gaatcggggt
     5821 ttctaggcat tttttaaata gtgacccagg actcaaaaac tagctctcaa cttctctcat
     5881 atgttgtgga gtctggagtc ctttatatat gtttacgcta tagaaggcat ttggggactc
     5941 tcccgggccc accataggac tccataccgc ggccatctat ataatattac tgatggccgc
     6001 gcggattttg gagccccctt ttgtcgttag cgcgaccttt aattcaaatt aaaggttgct
     6061 tttttctatc gtccgatcat tttgcgcctg actagcctag atattagcaa caacttcgcg
     6121 gctaagttgt tgaccgtcaa gtataaatta atgggcttat ggcctaatgg gtatagagca
     6181 aaatggcatg cctgcagact agtgctttac tctgatccgc agatatttca tcaggaaaga
     6241 gctttgcaag ttccttcatt gtagcatcaa taatttctga gtcactacat gcaatccatt
     6301 cttctgctgg ggcaaaaact aactccaaca tggattggtt tggattgtaa tattccttac
     6361 atgttacaga catgtcagca taaacactta aaagcgggct tctgctaaag agtagatgat
     6421 cataggtgtt cttcaatttc ctatcaaacc agatgtgaac gttgataact ggaactccaa
     6481 ctaatttctc taatttcttg aagtatgaaa tctctctcca gtcttcaggc actagtgaat
     6541 tcgctagcgt taactggcca taatcctgtg tatgcaacgt tgaaaattcg gatctatttt
     6601 tacgattcga tcatgaatta ataaaattta aattttattg aatgattttc tagtacataa
     6661 ctgacatacg acttatcagt tgcgaaacga acagctctaa ttacattgtt aagtgaaatt
     6721 acgcctaatt cgtctaagta caacataacc aactgtttaa acctaattaa ataagtcgac
     6781 ccagaagctg tcagagatgt cgtccagact tggaagttca ggtaagcttt gtggagatgc
     6841 aacgctctcc gtaagttgtg gttgaatcgg atctggacgt ggtatatacg cgtcctggtg
     6901 tatagcagat cctctactcc gtatatcttg aaatataggg gatttattat ctcccagata
     6961 tacacgccat tctctgcctg acgtgcagtg atgatttccc ctgtgcgtga atccatgacc
     7021 ggtgcagcct atgtggaagt atatggtgca cccacagttt agatcaatgc gtcgtcgtct
     7081 aatcgccctc ctcttggctt gcctgtgtgc cttcttgata gaggggggcg tcgagagtga
     7141 tgaagataac attcttgaga gtccaatgtt tgagcgacgc attttccgct ttatccagga
     7201 aatctttata gctggatccc tcgcctggat tgcaaagcac gattgaaggg atccctcctt
     7261 taatttgaac tggtttcccg tacttgcaat ttgactgcca gtctttttgg gccccaagaa
     7321 gctctttcca atgctttagc tttaggtatt gcggtgctac gtcatcaatg acgttatagg
     7381 caacattatt cgagtacact cgattattga agtccaggtg tccactgagg taattatgtg
     7441 ggcctaaagc acgagcccac atcgtcttgc ccgttctaga atcaccttcc actatgagac
     7501 taactggtct tacaggccgc gcagcgggac ccattccaaa ataatcatcc gcccattctt
     7561 gcatctgttc aggaacgtta gtgaaagagg aaagtggaaa tggagggacc cattgttcag
     7621 gcgccttttt gaaaaggcgt tccaagttcg ctgtaatgtt atggtaactc acgatgaacg
     7681 cccttggatc tccggcccgg ataattgcaa gagcttccgc caaactaggt gcattgatgg
     7741 cgttataata gacgtcgtct ttattcttct ttgtaccccc aggcactttg aactgtccgg
     7801 attcacaaca atcgccgtct ttggtgatgt aattcttgac ggcgttggcg tctttggctg
     7861 cttgtatgtt tgggtgaaaa ttggtcgacc ttctggggtg agtaaggtcg aaaaatctct
     7921 tattcttgat gttggacttt ccagagagtt ggatgagaca gtgaaggtgt gggaatccgt
     7981 ccgagtgctc ctctctggca actcgaatat atgttggttt gacgactgac catgataagg
     8041 tttgaagcat ttgaagagct tcattttttg ggatatcgca cttgggatag gttaagaaaa
     8101 tatgtctggc ttgtaaacga aaagaatcgg ggtttctagg cattttttaa atagtgaccc
     8161 aggactcaaa aactagctct caacttctct catatgttgt ggagtctgga gtcctttata
     8221 tatgtttacg ccatagaaag cattgggggt atagcatggt aatttcacat cggactccat
     8281 accgcggcca tctatataat attactgatg gccgcgcgga ttttggagcc cccttttgtc
     8341 gttagcgcga cctttaattc aaattaaagg ttgctttttt ctatcgtcca atcattttgc
     8401 gcctgactag cctagatatt agcaacaact tcgcgactaa gttgttgacc gtcaagtata
     8461 aattaatggg cttatggccc aatgggtata gagcaaaatg cccaagcgtg atgccccgtg
     8521 gcgcttatta gcgggtacgt ctaaggtaag tcgaaatgcc aactactctc ccttgagagt
     8581 attgggccct aaagttaata agggacctcg agggggggcc cggtacccgg ggatcctcta
     8641 gagtcgacct gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     8701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8821 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     8881 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     8941 aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     9001 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     9061 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     9121 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     9181 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     9241 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     9301 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     9361 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     9421 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     9481 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     9541 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     9601 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     9661 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     9721 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     9781 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     9841 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     9901 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     9961 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
    10021 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
    10081 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
    10141 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
    10201 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
    10261 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
    10321 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
    10381 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
    10441 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
    10501 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
    10561 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
    10621 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
    10681 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
    10741 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
    10801 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
    10861 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
    10921 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
    10981 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
    11041 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
    11101 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
    11161 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
    11221 tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
    11281 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
    11341 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
    11401 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
    11461 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
    11521 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
    11581 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
    11641 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
    11701 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
    11761 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
    11821 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
    11881 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
    11941 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
    12001 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
    12061 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
    12121 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
    12181 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
    12241 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
    12301 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
    12361 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtgtctttcc tgtggatagc
    12421 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
    12481 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
    12541 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
    12601 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
    12661 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
    12721 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
    12781 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
    12841 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    12901 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
    12961 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    13021 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    13081 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    13141 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    13201 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    13261 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    13321 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    13381 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    13441 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    13501 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    13561 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    13621 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    13681 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    13741 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    13801 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    13861 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    13921 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    13981 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    14041 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    14101 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    14161 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    14221 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    14281 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    14341 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    14401 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    14461 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    14521 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    14581 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    14641 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    14701 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    14761 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    14821 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    14881 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    14941 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    15001 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    15061 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    15121 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata
    15181 ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat
    15241 gtactgaatt aacgccgaat taattcgggg atagcccttt ggtcttctga gactgtatct
    15301 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc
    15361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    15421 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    15481 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    15541 gcggataaca atttcacaca ggaaacagct atgacatgat tacg
//
