LOCUS       sequence-476166-        3106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Universal acceptor vector with mScarlet-I placeholder for lvl0 
            assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_G_E_004_UAV_mScarlet-I
FEATURES             Location/Qualifiers
     source          1..3106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             163..174
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      885..956
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      972..999
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1050..1107
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1131..1148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1302..1890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1382..1401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2073..2167)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2188..2847)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2762..2781
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3009..3027
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3031..3074)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3077..3098
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtga gacggaaagt gaaacgtgat ttcatgcgtc attttgaaca ttttgtaaat
       61 cttatttaat aatgtgtgcg gcaattcaca tttaatttat gaatgttttc ttaacatcgc
      121 ggcaactcaa gaaacggcag gttcggatct tagctactag agaaagagga gaaatactag
      181 atgcgtaaag gcgaagcagt gatcaaagaa tttatgcgct tcaaagttca catggaaggt
      241 tctatgaacg gccacgaatt tgaaattgaa ggtgaaggcg aaggtcgtcc atacgaaggt
      301 actcaaaccg caaaactgaa agttaccaaa ggtggtccac tgccattttc ttgggatatt
      361 ctgtctccac aatttatgta cggttctcgt gcatttatca aacacccagc agatattcca
      421 gactactaca aacaatcttt tccggaaggt ttcaaatggg aacgtgttat gaattttgaa
      481 gatggtggtg cagttacggt tacccaagat acctctctgg aagatggtac tctgatctac
      541 aaagttaaac tgcgtggtac taactttcca ccagatggtc cagttatgca gaaaaaaacc
      601 atgggttggg aagcatctac cgaacgtctg tacccagaag atggcgttct gaaaggtgat
      661 atcaaaatgg cactgcgtct gaaagatggc ggtcgttacc tggcagattt caaaaccacc
      721 tacaaagcga aaaaaccagt tcaaatgcca ggtgcataca acgttgatcg taaactggat
      781 attaccagcc acaacgaaga ttacaccgtt gttgaacaat acgaacgttc tgaaggccgt
      841 cactctaccg gtggtatgga tgaactgtac aaataaccag gcatcaaata aaacgaaagg
      901 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      961 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatacgtct catgagacca
     1021 ctcatcgcga ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc
     1081 tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact
     1141 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1201 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     1861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     1921 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     1981 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2041 gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg
     2101 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     2161 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcgt cgcagtactg
     2221 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     2281 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     2341 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     2401 gggattggct gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     2461 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     2521 gtattcactc cagagtgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     2581 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     2641 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2701 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2761 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2821 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2881 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2941 gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     3001 cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
     3061 ctaaggatga tttctggaat tcgcggccgc ttctagagcg atgagt
//
