LOCUS       sequence-476175-        2573 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  chlL-rpl36 IEE Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3IEE_003_chlL-rpl36_IEE_Cr
FEATURES             Location/Qualifiers
     source          1..2573
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      517..574
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(598..615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(769..1357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(849..868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1540..1634)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1655..2314)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2229..2248
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2476..2494
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2498..2541)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2544..2565
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgtac ttaagaataa agcagcttta aatactttcc tgtttataat ttaggaaatt
       61 aaatggatat ttgttgaaac taatccccag ttggataccc attggtagtt aattgccact
      121 gcctgcttca ccttacaaaa tgtatggaca caaaacggct aataaataca gactcccggt
      181 ggcatttgtt ggctgcttcg ccctgaaagg agaaagtgat ttctttcctt attagctaat
      241 ctattctttt tcctgttttg gtaaataata gcgtcctcat atccatatct ataacaaaaa
      301 gttaaatgtt ttaattttaa aagtcttttt tacattaata aacactttta attgatggga
      361 catctttagt ttttaaaata aataaagatg caccctcaaa ctgtcagttc attaacccaa
      421 attatttaat ttggatgcat ataagcgaat tgacttcaaa actaataaaa tttttaatgt
      481 gagaccactc atcgcgacta gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc
      541 accaccctgc cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc
      601 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
      661 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
      721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
      781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1501 tatatatgag taaacttggt ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc
     1561 ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc
     1621 tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc cactcgtcgc
     1681 agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa acggcatgat
     1741 gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatgg
     1801 tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac
     1861 tcacccaggg attggctgat acgaaaaaca tattctcaat aaacccttta gggaaatagg
     1921 ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac tgccggaaat
     1981 cgtcgtggta ttcactccag agtgatgaaa acgtttcagt ttgctcatgg aaaacggtgt
     2041 aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc atacgaaatt
     2101 ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct
     2161 tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg
     2221 tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat
     2281 caacggtggt atatccagtg atttttttct ccattttagc ttccttagct cctgaaaatc
     2341 tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga aagttggaac
     2401 ctcttacgtg cccgatcaac tcgagtgcca cctgacgtct aagaaaccat tattatcatg
     2461 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
     2521 gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagcgatg agt
//
