LOCUS       sequence-47618-e         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ212 - pDESTR4-R3(cxTi10816, IV)
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(91..97,98..115,116..121))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(146..269)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(277..294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(277..293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
       61 atctcgacgg atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa
      121 agcctggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta
      181 tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat cattttacgt
      241 ttctcgttca actttattat acatagttga taattcactg gccgtcgttt tacactcgag
      301 acgtacggtg cgcgcgatgc attcgaagat ctgcccacta gtgagtcgta ttataagtgc
      361 aagtaagatc agtgtttgta tttaagacac acggccaaat ttcttatttt cctgctattt
      421 tctccaaata tgattaaaat ttgttcattt caaaattgcc tccaacttgt tttggggcaa
      481 aaatttcaca aaacttcaca gcaaaatgtg aaacaatatt gctatctatt gaaaacatta
      541 atgatagtcg agtgacttct tgaaccaata ctcatcgcat caaatgcctt ttctcgaata
      601 ctctgtaata attggcatat aaa
//
