LOCUS       sequence-476180-       15463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one subcellar localization vector. Construct containing EGFP 
            tag and plasma membrane-targeted mCherry for co-localization study 
            in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15463)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 15463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTCL2
FEATURES             Location/Qualifiers
     source          1..15463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1164..1874)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1442..1461)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2743..3088)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3118..3292
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(3300..4013,4014..4016,4017..4019))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3303..4013,4014..4016,4017..4019))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3345..3366)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3694..3713
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3953..3974
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(4103..4779)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8549..8566)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8549..8565)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8558..8580)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8768..8792
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10093..10722
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11151..12224
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12290..12484
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12720..12742)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12828..12968
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12883..12902
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12983..13000)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13154..13742)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13234..13253)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13829..14623)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    15048..15072
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    15340..15361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15376..15381,15382..15399,15400..15406)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15414..15430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15419..15441
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15438..15454
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15438..15454
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcggtaccct agattacttg tacagctcgt ccatgccgcc ggtggagtgg
     1201 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
     1261 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
     1321 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
     1381 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
     1441 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
     1501 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
     1561 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
     1621 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
     1681 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
     1741 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
     1801 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg
     1861 cccttgctca ccatgacgtt ggcagcactt ctgaatgatc cgagagactt agaacctgaa
     1921 gctctcagaa cgaattggtg gtagaatgca gctatcgcag ctccaatgaa tggtccaacc
     1981 caaaatatcc agtggtcatc ccatggcttg ctcttgttgt agattacggc agctccgaaa
     2041 ctccttgccg ggttaattcc ggttccggta atgggaatgg tagccaaatg taccatgaac
     2101 acggcaaatc cgattggaag tggcgccaac accggaacgt gggagtctct agcactacgt
     2161 ttggggtcag tagcagagaa gacagtgtag acaagaacga aagtaccaat gatctctgcg
     2221 gctaaaccgg tccctgtgct gtagccatcg gctagagagt tggctccacc tccgtaacgg
     2281 gtgtagtaag agctttggaa ggctttgaca aaaccaactc cacaaatcgc acccaaacac
     2341 tgagcgatta tgtacaatag ggccctaggt aacgacactt tacgtgccaa gaatagccca
     2401 aatgtcaccg ctgggttaat gtgaccacca gagataccgg cggtgcagta gacgaggatg
     2461 aagatcatac caccaaaggc ccaagcgata ccgaggattc caactccgcc gcaatctact
     2521 ccaccggcat cagtatcgga ctgaatcttg taaccgatga ctgtcaaaac ggtgatgtat
     2581 aagaagagga gagtggctac gaactcggcg ataactgctc tgtagaaaga ccacttcttt
     2641 agctccgctc catcaataaa cggagctggt ggcggatctt gatagtctct tgtctgaaat
     2701 ccttctccgg gaacggcttc cacatccttt gccatggtac cctgtcctct ccaaatgaaa
     2761 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     2821 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     2881 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     2941 atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
     3001 ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga ggaggtttcc
     3061 ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga ctgtatcgat
     3121 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
     3181 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
     3241 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct
     3301 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     3361 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     3421 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     3481 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     3541 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     3601 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     3661 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     3721 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     3781 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     3841 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     3901 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     3961 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     4021 agacctacgg tctcggatct cattgccccc cggatctgcg aaagctcgag agagatagat
     4081 ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata
     4141 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     4201 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     4261 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     4321 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     4381 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     4441 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     4501 agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt
     4561 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     4621 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     4681 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     4741 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     4801 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc
     4861 tgctctagct cacgttgacg gaaactgcag aacgatctca gttaggtcat gggaaagctg
     4921 atattcatcc ctctgagact ctatataatt aaatgcgttt gggggattag ctaattgaga
     4981 atccatatag agaaatatgg ccgcgcagcg acgccggttg aagaatatga acaaggaata
     5041 agatgaacaa ggaagaagat gaacaagcaa taatttaatt aattattgtt aataagcata
     5101 aattatgaaa ataattttga tggtaatgaa agtattataa ataatattta ggagaaaata
     5161 ttgttaagga aatatctggt tatgtttatt tgttaaggta tatgggtttt tatagaaacc
     5221 caggagcagc tatggtaaga aacccaggac tcaaaaaatg cctctcaact tctctcatat
     5281 gttgcggagt ctggagtccc atttatacca tagaaagcat tgggggtata gcatggtaat
     5341 ttcacatcgg actccatacc gcggccatct atataatatt actgatggcc gcgcgaagtc
     5401 ccggtatgcc ttttcagccg ttggatctgg agccgtacct ttttcgcgtt cccattttgc
     5461 ccctggagtc ccgagctatt tacgaccccc tgccaccgag tccccttcgt ggagcgctct
     5521 tcgtccaaca cgtgtcccaa cttttgcgca tttttgacta cctgtcgcgc acttgaatta
     5581 aagttgccct tttattttct tccaattatt ttgcggctga gtagtctagt ggatcccccg
     5641 gagctccact tgggataggt taagaaaata tgtctggctt gtaaacgaaa agaatcgggg
     5701 tttctaggca ttttttaaat agtgacccag gactcaaaaa ctagctctca acttctctca
     5761 tatgttgtgg agtctggagt cctttatata tgtttacgct atagaaggca tttggggact
     5821 ctcccgggcc caccatagga ctccataccg cggccatcta tataatatta ctgatggccg
     5881 cgcggatttt ggagccccct tttgtcgtta gcgcgacctt taattcaaat taaaggttgc
     5941 ttttttctat cgtccgatca ttttgcgcct gactagccta gatattagca acaacttcgc
     6001 ggctaagttg ttgaccgtca agtataaatt aatgggctta tggcctaatg ggtatagagc
     6061 aaaatggcat gcctgcagac tagtgcttta ctctgatccg cagatatttc atcaggaaag
     6121 agctttgcaa gttccttcat tgtagcatca ataatttctg agtcactaca tgcaatccat
     6181 tcttctgctg gggcaaaaac taactccaac atggattggt ttggattgta atattcctta
     6241 catgttacag acatgtcagc ataaacactt aaaagcgggc ttctgctaaa gagtagatga
     6301 tcataggtgt tcttcaattt cctatcaaac cagatgtgaa cgttgataac tggaactcca
     6361 actaatttct ctaatttctt gaagtatgaa atctctctcc agtcttcagg cactagtgaa
     6421 ttcgctagcg ttaactggcc ataatcctgt gtatgcaacg ttgaaaattc ggatctattt
     6481 ttacgattcg atcatgaatt aataaaattt aaattttatt gaatgatttt ctagtacata
     6541 actgacatac gacttatcag ttgcgaaacg aacagctcta attacattgt taagtgaaat
     6601 tacgcctaat tcgtctaagt acaacataac caactgttta aacctaatta aataagtcga
     6661 cccagaagct gtcagagatg tcgtccagac ttggaagttc aggtaagctt tgtggagatg
     6721 caacgctctc cgtaagttgt ggttgaatcg gatctggacg tggtatatac gcgtcctggt
     6781 gtatagcaga tcctctactc cgtatatctt gaaatatagg ggatttatta tctcccagat
     6841 atacacgcca ttctctgcct gacgtgcagt gatgatttcc cctgtgcgtg aatccatgac
     6901 cggtgcagcc tatgtggaag tatatggtgc acccacagtt tagatcaatg cgtcgtcgtc
     6961 taatcgccct cctcttggct tgcctgtgtg ccttcttgat agaggggggc gtcgagagtg
     7021 atgaagataa cattcttgag agtccaatgt ttgagcgacg cattttccgc tttatccagg
     7081 aaatctttat agctggatcc ctcgcctgga ttgcaaagca cgattgaagg gatccctcct
     7141 ttaatttgaa ctggtttccc gtacttgcaa tttgactgcc agtctttttg ggccccaaga
     7201 agctctttcc aatgctttag ctttaggtat tgcggtgcta cgtcatcaat gacgttatag
     7261 gcaacattat tcgagtacac tcgattattg aagtccaggt gtccactgag gtaattatgt
     7321 gggcctaaag cacgagccca catcgtcttg cccgttctag aatcaccttc cactatgaga
     7381 ctaactggtc ttacaggccg cgcagcggga cccattccaa aataatcatc cgcccattct
     7441 tgcatctgtt caggaacgtt agtgaaagag gaaagtggaa atggagggac ccattgttca
     7501 ggcgcctttt tgaaaaggcg ttccaagttc gctgtaatgt tatggtaact cacgatgaac
     7561 gcccttggat ctccggcccg gataattgca agagcttccg ccaaactagg tgcattgatg
     7621 gcgttataat agacgtcgtc tttattcttc tttgtacccc caggcacttt gaactgtccg
     7681 gattcacaac aatcgccgtc tttggtgatg taattcttga cggcgttggc gtctttggct
     7741 gcttgtatgt ttgggtgaaa attggtcgac cttctggggt gagtaaggtc gaaaaatctc
     7801 ttattcttga tgttggactt tccagagagt tggatgagac agtgaaggtg tgggaatccg
     7861 tccgagtgct cctctctggc aactcgaata tatgttggtt tgacgactga ccatgataag
     7921 gtttgaagca tttgaagagc ttcatttttt gggatatcgc acttgggata ggttaagaaa
     7981 atatgtctgg cttgtaaacg aaaagaatcg gggtttctag gcatttttta aatagtgacc
     8041 caggactcaa aaactagctc tcaacttctc tcatatgttg tggagtctgg agtcctttat
     8101 atatgtttac gctatagaag gcatttgggg actctcccgg gcccaccata ggactccata
     8161 ccgcggccat ctatataata ttactgatgg ccgcgcggat tttggagccc ccttttgtcg
     8221 ttagcgcgac ctttaattca aattaaaggt tgcttttttc tatcgtccaa tcattttgcg
     8281 cctgactagc ctagatatta gcaacaactt cgcgactaag ttgttgaccg tcaagtataa
     8341 attaatgggc ttatggccca atgggtatag agcaaaatgc ccaagcgtga tgccccgtgg
     8401 cgcttattag cgggtacgtc taaggtaagt cgaaatgcca actactctcc cttgagagta
     8461 ttgggcccta aagttaataa gggacctcga gggggggccc ggtacccggg gatcctctag
     8521 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     8581 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8641 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8701 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat
     8761 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     8821 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     8881 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     8941 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     9001 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     9061 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     9121 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     9181 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     9241 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     9301 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     9361 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     9421 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     9481 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     9541 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     9601 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     9661 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     9721 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     9781 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     9841 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     9901 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     9961 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
    10021 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
    10081 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
    10141 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
    10201 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
    10261 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
    10321 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
    10381 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
    10441 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
    10501 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
    10561 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
    10621 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
    10681 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
    10741 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
    10801 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
    10861 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
    10921 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
    10981 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
    11041 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
    11101 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
    11161 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
    11221 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
    11281 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
    11341 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
    11401 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
    11461 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
    11521 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
    11581 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
    11641 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
    11701 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
    11761 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
    11821 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
    11881 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
    11941 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
    12001 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
    12061 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
    12121 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    12181 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
    12241 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tgtctttcct gtggatagca
    12301 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
    12361 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
    12421 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
    12481 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
    12541 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
    12601 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
    12661 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    12721 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    12781 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    12841 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
    12901 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    12961 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    13021 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    13081 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    13141 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    13201 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    13261 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    13321 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    13381 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    13441 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    13501 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    13561 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    13621 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    13681 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    13741 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    13801 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
    13861 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    13921 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    13981 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    14041 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    14101 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    14161 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    14221 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    14281 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    14341 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    14401 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    14461 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    14521 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    14581 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    14641 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    14701 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    14761 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    14821 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    14881 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    14941 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    15001 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    15061 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    15121 tactgaatta acgccgaatt aattcgggga tagccctttg gtcttctgag actgtatctt
    15181 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
    15241 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    15301 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    15361 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    15421 cggataacaa tttcacacag gaaacagcta tgacatgatt acg
//
