LOCUS       sequence-476187-       17598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human VPS13C^Halo in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17598)
  AUTHORS   Wang X, Xu P, Bentley-DeSousa A, Hancock-Cerutti W, Cai S, Johnson 
            BT, Tonelli F, Shao L, Talaia G, Alessi DR, Ferguson SM, De Camilli 
            P
  TITLE     The bridge-like lipid transport protein VPS13C/PARK23 mediates 
            ER-lysosome contacts following lysosome damage.
  JOURNAL   Nat Cell Biol. 2025 May;27(5):776-789. doi: 
            10.1038/s41556-025-01653-6. Epub 2025 Apr 10.
  PUBMED    40211074
REFERENCE   2  (bases 1 to 17598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-025-01653-6"; journalName: "Nat Cell Biol"; date: 
            "2025-05"; volume: "27"; issue: "5"; pages: "776-789"
COMMENT     Sequence Label: VPS13C^Halo
FEATURES             Location/Qualifiers
     source          1..17598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      915..924
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6672..7559
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     primer_bind     complement(13189..13206)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    13195..13419
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13465..13893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13552..13571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13762..13783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13902..13922)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13907..14236
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14087..14222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14149..14168
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             14303..15097
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14357..14376)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14967..14986
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15271..15392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15308..15327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15362..15381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15441..15457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15441..15457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15454..15476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15465..15481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15489..15495,15496..15513,15514..15519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15534..15555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15672..15689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15843..16431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15923..15942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16602..17393,17394..17462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17225..17244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17463..17567)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gcccgccacc atggtcctgg agagcgtggt cgccgatctg ctgaaccgat
      961 tcctggggga ttacgtggag aacctgaaca agtcacagct gaaactggga atctggggag
     1021 gaaacgtggc cctggacaat ctgcagatca aggagaacgc cctgagcgag ctggacgtgc
     1081 ccttcaaggt gaaggccggc cagatcgata agctgaccct gaagatccct tggaagaatc
     1141 tgtacggaga ggcagtggtg gccacactgg agggcctgta tctgctggtg gtgccaggag
     1201 ccagcatcaa gtacgacgcc gtgaaggagg agaagtctct gcaggatgtg aagcagaagg
     1261 agctgagccg gatcgaggag gccctgcaga aggcagcaga gaagggaacc cactccggag
     1321 agtttatcta tggcctggag aacttcgtgt acaaggacat caagcctggc cgcaagcgga
     1381 agaagcacaa gaagcacttt aagaagcctt tcaagggcct ggacagatcc aaggataagc
     1441 caaaggaggc caagaaggat accttcgtgg agaagctggc cacacaggtc atcaagaatg
     1501 tgcaggtgaa gatcacagac atccacatca agtatgagga cgatgtgacc gatccaaaga
     1561 ggcccctgag ctttggcgtg acactgggcg agctgtccct gctgacagcc aacgagcact
     1621 ggaccccatg catcctgaat gaggccgaca agatcatcta caagctgatc cgcctggata
     1681 gcctgtccgc ctattggaac gtgaattgtt ctatgagcta ccagagatcc agggagcaga
     1741 tcctggacca gctgaagaac gagatcctga cctctggcaa catcccccct aattaccagt
     1801 atatcttcca gcctatctcc gcctctgcca agctgtatat gaatccatac gccgagagcg
     1861 agctgaagac ccccaagctg gattgcaaca tcgagatcca gaatatcgcc atcgagctga
     1921 caaagccaca gtatctgtct atgatcgacc tgctggagag cgtggattac atggtgagga
     1981 acgcccccta ccgcaagtat aagccttacc tgccactgca caccaatggc cggagatggt
     2041 ggaagtatgc catcgactct gtgctggagg tgcacatcag gcgctacaca cagatgtgga
     2101 gctggtccaa catcaagaag caccggcagc tgctgaagtc ctataagatc gcctacaaga
     2161 ataagctgac ccagtccaag gtgtctgagg agatccagaa ggagatccag gacctggaga
     2221 agacactgga cgtgttcaac atcatcctgg caaggcagca ggcacaggtg gaagtgatca
     2281 gatccggcca gaagctgagg aagaagtctg ccgacaccgg agagaagaga ggaggatggt
     2341 tcagcggcct gtggggcaag aaggagagca agaagaagga cgaggagtct ctgatccctg
     2401 agacaatcga cgatctgatg accccagagg agaaggataa gctgtttacc gccatcggct
     2461 attctgagag cacacacaat ctgaccctgc ccaagcagta cgtggcccac atcatgacac
     2521 tgaagctggt gtccacctct gtgacaatca gggagaacaa gaatatccct gagatcctga
     2581 agatccagat catcggcctg ggaacccagg tgtcccagcg gccaggcgcc caggccctga
     2641 aggtggaggc aaagctggag cactggtata tcacaggcct gagacagcag gacatcgtgc
     2701 ctagcctggt ggcctccatc ggcgatacca caagctccct gctgaagatc aagttcgaga
     2761 caaaccccga ggactcccct gccgatcaga ccctgatcgt gcagtctcag ccagtggaag
     2821 tgatctacga cgccaagacc gtgaacgccg tggtggagtt ctttcagtct aataagggcc
     2881 tggatctgga gcagatcacc agcgccacac tgatgaagct ggaggagatc aaggagcgca
     2941 ccgccacagg cctgacacac atcatcgaga cacgcaaggt gctggacctg cggatcaacc
     3001 tgaagccttc ctatctggtg gtgccacaga ccggcttcca ccacgagaag tctgacctgc
     3061 tgatcctgga ttttggcaca ttccagctga acagcaagga ccagggcctg cagaagacca
     3121 caaattctag cctggaggag atcatggata aggcctacga caagtttgat gtggagatca
     3181 agaatgtgca gctgctgttc gccagagccg aggagacatg gaagaagtgc aggtttcagc
     3241 acccaagcac aatgcacatc ctgcagccca tggacatcca cgtggagctg gccaaggcca
     3301 tggtggagaa ggatatcaga atggcaaggt tcaaggtgtc cggaggcctg ccactgatgc
     3361 acgtgaggat ctctgaccag aagatgaagg atgtgctgta tctgatgaat agcatcccac
     3421 tgccccagaa gtcctctgcc cagtcccctg agaggcaggt gagctccatc ccaatcatca
     3481 gcggaggaac aaagggcctg ctgggcacct ccctgctgct ggacacagtg gagagcgagt
     3541 ccgacgatga gtacttcgac gcagaggatg gagagccaca gacctgtaag tctatgaagg
     3601 gcagcgagct gaagaaggcc gccgaggtgc ctaacgagga gctgatcaat ctgctgctga
     3661 agtttgagat caaggaagtg atcctggagt tcaccaagca gcagaaggag gaggatacca
     3721 tcctggtgtt taacgtgaca cagctgggca ccgaggccac aatgaggacc ttcgacctga
     3781 cagtggtgtc ttatctgaag aagatcagcc tggattacca cgagatcgag ggcagcaagc
     3841 gcaagcccct gcacctgatc tctagctccg acaagcctgg cctggatctg ctgaaggtgg
     3901 agtatatcaa ggccgacaag aatggccctt cctttcagac cgccttcggc aagacagagc
     3961 agaccgtgaa ggtggccttc tctagcctga acctgctgct gcagacacag gccctggtgg
     4021 cctctatcaa ttacctgacc acaatcatcc ctagcgacga tcagtctatc agcgtggcca
     4081 aggaggtgca gatcagcacc gagaagcagc agaagaactc cacactgcca aaggccatcg
     4141 tgtcctctag agacagcgat atcatcgact ttaggctgtt cgccaagctg aacgcctttt
     4201 gcgtgatcgt gtgcaatgag aagaacaata tcgccgagat caagatccag ggcctggata
     4261 gctccctgag cctgcagtcc agaaagcagt ccctgttcgc caggctggag aatatcatcg
     4321 tgaccgacgt ggatcccaag acagtgcaca agaaggccgt gtctatcatg ggcaacgagg
     4381 tgttcaggtt taatctggac ctgtatcctg atgccaccga gggcgacctg tacacagata
     4441 tgagcaaggt ggacggcgtg ctgtccctga acgtgggctg catccagatc gtgtacctgc
     4501 acaagttcct gatgagcctg ctgaattttc tgaacaattt ccagaccgcc aaggagtccc
     4561 tgtctgccgc aacagcacag gcagcagaga gggcagccac ctccgtgaag gacctggcac
     4621 agcggagctt ccgggtgagc atcaacatcg atctgaaggc ccccgtgatc gtgatccctc
     4681 agtctagcat cagcaccaat gccgtggtgg tggacctggg cctgatcaga gtgcacaacc
     4741 agttttctct ggtgagcgac gaggattatc tgaatccacc cgtgatcgac cgcatggatg
     4801 tgcagctgac aaagctgacc ctgtatcgga ccgtgatcca gccaggcatc taccaccccg
     4861 atatccagct gctgcaccct atcaacctgg agttcctggt gaaccggaat ctggccgcct
     4921 cctggtacca caaggtgcca gtggtggaga tcaagggcca cctggacagc atgaacgtgt
     4981 ccctgaatca ggaggatctg aacctgctgt ttaggatcct gaccgagaat ctgtgcgagg
     5041 gcacagagga cctggataag gtgaagcccc gcgtgcagga gacaggcgag atcaaggagc
     5101 ctctggagat cagcatctcc caggacgtgc acgattctaa gaacacactg accacaggcg
     5161 tggaggagat ccggagcgtg gacatcatca acatgctgct gaatttcgag atcaaggagg
     5221 tggtggtgac cctgatgaag aagagcgaga agaagggcag acccctgcac gagctgaatg
     5281 tgctgcagct gggcatggag gccaaggtga agacatatga tatgaccgcc aaggcctacc
     5341 tgaagaagat ctctatgcag tgtttcgact ttaccgatag caagggcgag cccctgcaca
     5401 tcatcaactc ctctaatgtg acagacgagc ctctgctgaa gatgctgctg accaaggccg
     5461 actctgatgg ccctgagttt aagacaatcc acgatagcac caagcagagg ctgaaggtgt
     5521 ccttcgcctc tctggacctg gtgctgcacc tggaggccct gctgagcttt atggatttcc
     5581 tgagctccgc cgcaccattc tccgagccct ctagctccga gaaggagtct gagctgaagc
     5641 cactggtggg cgagtctcgc agcatcgccg tgaaggccgt gtctagcaac atctcccaga
     5701 aggacgtgtt tgatctgaag atcaccgccg agctgaacgc cttcaacgtg ttcgtgtgcg
     5761 accagaagtg taatatcgcc gatatcaaga tccacggcat ggacgcctcc atctctgtga
     5821 agccaaagca gacagacgtg ttcgccaggc tgaaggacat catcgtgatg aacgtggatc
     5881 tgcagtctat ccacaagaag gccgtgagca tcctgggcga cgaggtgttc cgctttcagc
     5941 tgaccctgta tccagatgca acagagggag aggcatacgc agacatgtcc aaggtggatg
     6001 gcaagctgtc ttttaaggtc ggatgtatcc agatcgtgta cgtgcacaag ttctttatgt
     6061 ctctgctgaa ctttctgaat aacttccaga ccgcaaagga ggccctgagc accgcaacag
     6121 tgcaggccgc cgagagggca gcctcctcta tgaaggacct ggcccagaag tctttccgcc
     6181 tgctgatgga tatcaatctg aaggcccctg tgatcatcat cccacagagc tccgtgagcc
     6241 caaacgccgt gatcgcagac ctgggcctga ttagggtgga gaataagttt tctctggtgc
     6301 ctatggagca ctatagcctg cctccagtga tcgacaagat gaacatcgag ctgacccagc
     6361 tgaagctgag ccggacaatc ctgcaggcat ccctgccaca gaatgatatc gagatcctga
     6421 agcctgtgaa catgctgctg agcatccaga gaaatctggc agcagcatgg tacgtgcaga
     6481 tcccaggcat ggagatcaag ggcaagctga agcccatgca ggtggccctg tccgaggacg
     6541 atctgaccgt gctgatgaag atcctgctgg agaacctggg cgaggcctct agccagcctt
     6601 ctccaaccca gagcgtgcag gagacagtgc gcgtgcggaa ggtggacgtg tcctctgtgc
     6661 catccgcggg agcagagatc ggaaccggct tcccctttga tcctcactat gtggaggtgc
     6721 tgggcgagcg catgcactac gtggacgtgg gaccccggga cggaacacct gtgctgttcc
     6781 tgcacggcaa ccctaccagc tcctacgtgt ggcgcaatat catccctcac gtggcaccaa
     6841 cccacaggtg catcgcacca gacctgatcg gcatgggcaa gagcgacaag cccgatctgg
     6901 gctacttctt tgacgatcac gtgaggttta tggacgcctt catcgaggcc ctgggcctgg
     6961 aggaggtggt gctggtcatc cacgattggg gatccgccct gggattccac tgggcaaaga
     7021 gaaacccaga gagggtgaag ggcatcgcct ttatggagtt catcaggcca atccctacct
     7081 gggacgagtg gcccgagttt gccagagaga catttcaggc cttcagaacc acagacgtgg
     7141 gcaggaagct gatcatcgat cagaacgtgt tcatcgaggg cacactgcca atgggagtgg
     7201 tgcggcccct gaccgaggtg gagatggacc actataggga gccctttctg aatcctgtgg
     7261 atcgggagcc actgtggaga ttcccaaacg agctgccaat cgcaggagag ccagcaaata
     7321 tcgtggccct ggtggaggag tacatggact ggctgcacca gagcccagtg cccaagctgc
     7381 tgttctgggg caccccaggc gtgctgatcc cacctgcaga ggcagcaagg ctggccaagt
     7441 ccctgccaaa ctgtaaggcc gtggatatcg gccctggcct gaacctgctg caggaggaca
     7501 atcccgatct gatcggctct gagatcgcca gatggctgag caccctggag atcagcggat
     7561 ccgcgggaga ccacctgaag gagcaggagg actggacaga tagcaagctg tccatgaatc
     7621 agatcgtgag cctgcagttc gattttcact ttgagtctct gagcatcatc ctgtacaaca
     7681 atgacatcaa ccaggagtcc ggcgtggcct ttcacaatga ttctttccag ctgggcgagc
     7741 tgaggctgca cctgatggcc tctagcggca agatgttcaa ggacggctcc atgaacgtgt
     7801 ctgtgaagct gaagacctgc acactggacg atctgaggga gggaatcgag agggccacct
     7861 cccggatgat cgatagaaag aacgaccagg ataacaattc ctctatgatc gacatcagct
     7921 ataagcagga taagaatggc tcccagatcg acgccgtgct ggataagctg tacgtgtgcg
     7981 cctctgtgga gtttctgatg accgtggccg acttctttat caaggcagtg ccacagagcc
     8041 cagagaacgt ggccaaggag acacagatcc tgcctaggca gaccgccaca ggcaaggtga
     8101 agatcgagaa ggacgatagc gtgcgcccta atatgaccct gaaggccatg atcacagacc
     8161 cagaggtggt gtttgtggca tccctgacca aggcagatgc accagccctg acagcctctt
     8221 tccagtgcaa cctgtccctg tctaccagca agctggagca gatgatggag gcatccgtgc
     8281 gggacctgaa ggtgctggcc tgtccatttc tgagagagaa gaggggcaag aatatcacca
     8341 cagtgctgca gccctgctcc ctgttcatgg agaagtgtac ctgggcctct ggcaagcaga
     8401 acatcaatat catggtgaag gagttcatca tcaagatcag ccctatcatc ctgaacacag
     8461 tgctgaccat catggccgcc ctgtccccaa agaccaagga ggacggcagc aaggatacat
     8521 ccaaggagat ggagaatctg tggggcatca agagcatcaa cgactacaat acctggtttc
     8581 tgggcgtgga taccgccaca gagatcacag agtccttcaa gggcatcgag cactctctga
     8641 tcgaggagaa ctgcggcgtc gtggtggaga gcatccaggt gacactggag tgtggcctgg
     8701 gacacagaac cgtgccactg ctgctggccg agtctaagtt cagcggcaac atcaagaatt
     8761 ggacatccct gatggcagca gtggcagacg tgaccctgca ggtgcactac tataatgaga
     8821 tccacgccgt gtgggagcct ctgatcgaga gggtggaggg caagcgccag tggaacctgc
     8881 ggttggacgt gaagaagaat cccgtgcagg ataagagcct gctgcctggc gacgacttca
     8941 tccctgagcc acagatggcc atccacatca gctccggcaa cacaatgaat atcaccatct
     9001 ccaagtcttg cctgaacgtg tttaacaatc tggccaaggg cttctccgag ggcacagcct
     9061 ctacctttga ctatagcctg aaggatagag cccccttcac cgtgaagaat gccgtgggcg
     9121 tgccaatcaa ggtgaagccc aactgtaatc tgcgcgtgat gggctttcct gagaagagcg
     9181 acatcttcga cgtggatgcc ggccagaacc tggagctgga gtacgcctcc atggtgccat
     9241 ctagccaggg caatctgtcc atcctgtcta ggcaggagtc ctctttcttt accctgacaa
     9301 tcgtgcccca cggctatacc gaggtggcca acatccctgt ggccagacca ggccggagac
     9361 tgtacaatgt gaggaacccc aatgccagcc actccgactc tgtgctggtg cagatcgatg
     9421 ccaccgaggg caacaaagtg atcaccctgc gcagccctct gcagatcaag aatcactttt
     9481 ccatcgcctt catcatctat aagttcgtga agaacgtgaa gctgctggag agaatcggca
     9541 tcgccaggcc tgaggaggag tttcacgtgc cactggacag ctacaggtgc cagctgttca
     9601 tccagccagc cggcatcctg gagcaccagt ataaggagtc caccacatac atctcttgga
     9661 aggaggagct gcacagaagc agggaggtgc gctgcatgct gcagtgtcca tctgtggagg
     9721 tgagctttct gcccctgatc gtgaacaccg tggccctgcc cgatgagctg tcctacatct
     9781 gtacacacgg cgaggactgg gatgtggcct atatcatcca cctgtaccct agcctgaccc
     9841 tgcgcaatct gctgccatat tccctgaggt acctgctgga gggaacagca gagacacacg
     9901 agctggcaga gggaagcaca gcagacgtgc tgcacagccg gatctccgga gagatcatgg
     9961 agctggtgct ggtgaagtac cagggcaaga actggaatgg ccactttcgc atccgggata
    10021 ccctgcccga gttctttccc gtgtgcttca gctccgactc cacagaggtg accacagtgg
    10081 atctgtctgt gcacgtgagg aggatcggca gccggatggt gctgagcgtg ttcagccctt
    10141 attggctgat caacaagacc acacgcgtgc tgcagtaccg gtccgaggac atccacgtga
    10201 agcacccagc cgactttaga gatatcatcc tgttcagctt caagaagaag aacatcttca
    10261 caaagaataa ggtgcagctg aagatcagca cctccgcctg gtctagctcc ttttccctgg
    10321 ataccgtggg ctcttatggc tgcgtgaagt gtcccgccaa caatatggag tacctggtgg
    10381 gcgtgtctat caagatgtct agctttaacc tgagcaggat cgtgaccctg acaccattct
    10441 gcaccatcgc caataagtcc tctctggagc tggaggtcgg agagatcgca tccgacggat
    10501 ctatgcccac aaacaagtgg aattatatcg ccagctccga gtgtctgcct ttctggccag
    10561 agtctctgag cggcaagctg tgcgtgcggg tggtgggatg tgagggctct agcaagccct
    10621 tcttttacaa cagacaggat aatggcaccc tgctgagcct ggaggacctg aacggcggca
    10681 tcctggtgga tgtgaataca gccgagcaca gcacagtgat caccttttcc gactatcacg
    10741 agggctctgc ccctgccctg atcatgaacc acaccccatg ggatatcctg acatacaagc
    10801 agtccggctc tcccgaggag atggtgctgc tgcctaggca ggcaaggctg tttgcatggg
    10861 cagaccctac cggaacaagg aagctgacct ggacatatgc agcaaacgtg ggagagcacg
    10921 acctgctgaa ggatggctgt ggccagttcc catacgacgc caatatccag atccactggg
    10981 tgagctttct ggatggcaga cagagggtgc tgctgttcac cgacgatgtg gccctggtgt
    11041 ccaaggccct gcaggcagag gagatggagc aggccgatta tgagatcaca ctgagcctgc
    11101 actccctggg cctgagcctg gtgaacaatg agagcaagca ggaggtgtcc tacatcggca
    11161 tcacctcctc tggcgtggtg tgggaggtga agccaaagca gaagtggaag cccttctccc
    11221 agaagcagat catcctgctg gagcagagct accagaagca ccagatctcc cgggaccacg
    11281 gctggatcaa gctggataac aattttgagg tgaacttcga caaggatccc atggagatga
    11341 ggctgccaat ccgctccccc atcaagaggg actttctgtc tggcatccag atcgagttca
    11401 agcagagctc ccaccagcgc agcctgcggg ccagactgta ctggctgcag gtggataacc
    11461 agctgcctgg cgccatgttt ccagtggtgt tccacccagt ggcaccacca aagtctatcg
    11521 ccctggacag cgagcccaag ccttttatcg acgtgagcgt gatcaccaga ttcaatgagt
    11581 attctaaggt gctgcagttt aagtacttca tggtgctgat ccaggagatg gccctgaaga
    11641 tcgaccaggg ctttctgggc gccatcatcg ccctgttcac ccctaccaca gatccagagg
    11701 ccgagcggag aaggacaaag ctgatccagc aggacatcga tgccctgaac gccgagctga
    11761 tggagacatc catgaccgac atgtctatcc tgagcttctt tgagcacttt cacatctctc
    11821 ccgtgaagct gcacctgagc ctgtccctgg gatccggagg cgaggagtct gataaggaga
    11881 agcaggagat gttcgccgtg cactctgtga atctgctgct gaagagcatc ggcgccaccc
    11941 tgacagacgt ggacgatctg atctttaagc tggcctacta tgagatccgc tatcagttct
    12001 acaagcggga tcagctgatc tggagcgtgg tgcggcacta ttccgagcag tttctgaagc
    12061 agatgtacgt gctggtgctg ggcctggacg tgctgggaaa ccccttcggc ctgatcagag
    12121 gcctgagcga gggagtggag gccctgtttt atgagccatt ccagggagca gtgcagggac
    12181 ccgaggagtt tgcagagggc ctggtcatcg gcgtgcggtc cctgttcgga cacaccgtgg
    12241 gaggagcagc aggagtggtg agcagaatca ccggatccgt gggcaagggc ctggcagcca
    12301 tcacaatgga caaggagtac cagcagaagc gccgggagga gctgagcaga cagcctaggg
    12361 actttggcga ttccctggcc agaggcggca agggcttcct gaggggagtg gtgggaggag
    12421 tgacaggcat catcaccaag ccagtggagg gagccaagaa ggagggagca gcaggcttct
    12481 ttaagggcat cggcaagggc ctggtgggag cagtggccag acccacaggc ggcatcgtgg
    12541 acatggcctc tagcaccttc cagggcatcc agagagccgc cgagagcacc gaggaggtgt
    12601 cctctctgcg ccctccaagg ctgatccacg aggacggcat catccggccc tatgatagac
    12661 aggagtccga gggctctgat ctgctggaga accacatcaa gaagctggag ggcgagacat
    12721 acagatatca ctgcgccatc cctggctcca agaagacaat cctgatggtg accaatagac
    12781 gggtgctgtg catcaaggag gtggagatcc tgggcctgat gtgcgtggac tggcagtgtc
    12841 cctttgagga tttcgtgttc cccccttctg tgagcgagaa cgtgctgaag atcagcgtga
    12901 aggagcaggg cctgttccac aagaaggact ccgccaatca gggctgcgtg agaaaggtgt
    12961 acctgaaaga caccgccaca gccgagaggg cctgtaacgc catcgaggat gcccagagca
    13021 cccgccagca gcagaaactg atgaaacaga gctccgtgag actgctgaga ccacagctgc
    13081 cttcctgact cgagcggccg ccactgtgct ggatatctgc agaattccac cacactggac
    13141 tagtggatcc gagctcggta ccaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc
    13201 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
    13261 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
    13321 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
    13381 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
    13441 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    13501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    13561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    13621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    13681 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    13741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    13801 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    13861 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
    13921 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    13981 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    14041 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    14101 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    14161 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    14221 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
    14281 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
    14341 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
    14401 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
    14461 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
    14521 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
    14581 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
    14641 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
    14701 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
    14761 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
    14821 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
    14881 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
    14941 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
    15001 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
    15061 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
    15121 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
    15181 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    15241 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    15301 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    15361 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    15421 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    15481 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    15541 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    15601 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    15661 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    15721 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    15781 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    15841 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    15901 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    15961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    16021 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    16081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    16141 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    16201 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    16261 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    16321 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    16381 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    16441 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    16501 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    16561 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    16621 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    16681 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    16741 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    16801 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    16861 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    16921 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    16981 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    17041 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    17101 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    17161 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    17221 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    17281 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    17341 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    17401 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    17461 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    17521 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    17581 aaagtgccac ctgacgtc
//
