LOCUS       sequence-476202-       10038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for knock-in of mRuby3-T2A-secNluc into the human 
            PECAM1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10038)
  AUTHORS   Zhao Y, Sun M, Pan Z, Kong W, Hong Z, Zhang W, Sun B, Zhang J, Wang 
            X, Wang K
  TITLE     A novel quantitative angiogenesis assay based on visualized vascular
            organoid.
  JOURNAL   Angiogenesis. 2025 Jan 3;28(1):10. doi: 10.1007/s10456-024-09967-z.
  PUBMED    39751990
REFERENCE   2  (bases 1 to 10038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Angiogenesis."; date: "2025-01-3"; volume: "28(1):10. doi"; pages: 
            " 10.1007/s10456-024-09967-z"
COMMENT     Sequence Label: PECAM1-mRuby3-T2A-secNluc
FEATURES             Location/Qualifiers
     source          1..10038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1414..1467
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1555..2067
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(2080..2097)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2086..2310
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2342..2375
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2436..2934
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2471..2494)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2867..2886
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2974..3573
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APHNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2974..2993)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     protein_bind    4130..4163
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    4890..5011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4901..4920)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4955..4974
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(5050..5763,5764..5766,5767..5769))
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from mClover2, with improved photostability (Bajar et al., 
                     2003)"
                     /label=mClover3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(5095..5116)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5703..5724
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5766..5775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5782..6960)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5791..6729
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5814..5834)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     complement(6984..7003)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6985..7003)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7010..7027)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7010..7026)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7019..7041)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             7222..8016
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7276..7295)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7886..7905
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(8034..8102,8103..8894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8252..8271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9065..9653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9554..9573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9807..9824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9941..9962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9977..9982,9983..10000,10001..10007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10015..10031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10020..10038,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttggtac cgagctcgtt ccaacatgta
       61 tgcagggaca gaggctatgg tccctacaga gcaggcatgg agagaaggag aaatacatac
      121 gggcaggagc caggagaggg agggtgtagt gagcagagac cgcgccactg cactccagcc
      181 tgagtgacag agtgagaatc catctaaaaa aaattgcttg ctaaagaagt ggtctcctga
      241 ggtcttaaga cgttcctgac aatgtcttga gtgggtggga gagaggctgc agtcattgtg
      301 ctgtggaatt tcagaggtga gaaccacacc taaccaaaat tactttccct gtttgcctca
      361 gtgacacagc tgcaggaacc ctggtgggtg ttgtattaag taaatttgac ctttattctt
      421 tgcagatctg tgaaatgttg tcttctgagg ggccacgtgt atctgtagtg ctgaggactc
      481 cttgggcctc tgaagtcaca gagagaaccc tgcagggtgg gggaccagtg tgtgacagcc
      541 ctgctttgca ttttctttga gaagtgctgt cattttgcat ttctctccac cagggcaatc
      601 ttcaatcttg agaggtgtga tcataacttg ccttgtttct tgtcgctaca gagaacggaa
      661 ggctcccttg atggaactgg tggttctggt ggtggttctg gtgtgagcaa gggcgaggag
      721 ctgatcaagg agaacatgag aatgaaggtg gtgatggagg gcagcgtgaa cggccatcag
      781 ttcaagtgca ccggcgaggg cgagggcaga ccctacgagg gcgtgcagac catgagaatc
      841 aaggtgatcg agggcggccc cctgcccttc gccttcgaca tcctggccac aagcttcatg
      901 tacggcagca gaaccttcat caagtacccc gccgacattc ccgacttctt caagcagagc
      961 ttccccgagg gcttcacctg ggagagagtg acaagatacg aggacggcgg cgtggtgacc
     1021 gtgacccaag acacaagcct ggaggacggc gagctggtgt acaacgtgaa ggtgagaggc
     1081 gtgaacttcc ctagcaacgg ccccgtgatg cagaagaaga ccaagggctg ggagcccaac
     1141 accgagatga tgtatcctgc cgatggcggc ctgagaggct acaccgacat cgccctgaag
     1201 gtggacggcg ggggccacct gcactgcaac ttcgtgacca cctacagaag caagaagacc
     1261 gtgggcaaca tcaagatgcc cggcgtgcac gccgtggacc acagactgga gagaatcgag
     1321 gagagcgaca acgagaccta cgtggtgcag agagaggtgg ccgtggccaa gtacagcaac
     1381 ctgggcgggg gcatggacga gctgtacaag aacgagggca gaggaagtct gctaacatgc
     1441 ggtgacgtcg aggagaatcc tggcccaatg aactccttct ccacaagcgc cttcggtcca
     1501 gttgccttct ccctgggcct gctcctggtg ttgcctgctg ccttccctgc cccagtcttc
     1561 acactcgaag atttcgttgg ggactggcga cagacagccg gctacaacct ggaccaagtc
     1621 cttgaacagg gaggtgtgtc cagtttgttt cagaatctcg gggtgtccgt aactccgatc
     1681 caaaggattg tcctgagcgg tgaaaatggg ctgaagatcg acatccatgt catcatcccg
     1741 tatgaaggtc tgagcggcga ccaaatgggc cagatcgaaa aaatttttaa ggtggtgtac
     1801 cctgtggatg atcatcactt taaggtgatc ctgcactatg gcacactggt aatcgacggg
     1861 gttacgccga acatgatcga ctatttcgga cggccgtatg aaggcatcgc cgtgttcgac
     1921 ggcaaaaaga tcactgtaac agggaccctg tggaacggca acaaaattat cgacgagcgc
     1981 ctgatcaacc ccgacggctc cctgctgttc cgagtaacca tcaacggagt gaccggctgg
     2041 cggctgtgcg aacgcattct ggcgtaaaac gctgatcagc ctcgactgtg ccttctagtt
     2101 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2161 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2221 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2281 ggcatgctgg ggatgcggtg ggctctatgg aagcttaacc ttctgaggcg gaaagaacca
     2341 gataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc
     2401 agccaagctt aggatctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag
     2461 gcagtctgga gcatgcgctt tagcagcccc gctggcactt ggcgctacac aagtggcctc
     2521 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     2581 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     2641 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     2701 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     2761 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     2821 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     2881 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     2941 atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc
     3001 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     3061 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3121 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3181 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3241 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3301 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3361 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3421 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgcccca caacctcccc
     3481 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     3541 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     3601 accgaaagga gcgcacgacc ccatgcatcg atgatatcag atccccggga tgcagaaatt
     3661 gatgatctat taaacaataa agatgtccac taaaatggaa gtttttcctg tcatactttg
     3721 ttaagaaggg tgagaacaga gtacctacat tttgaatgga aggattggag ctacgggggt
     3781 gggggtgggg tgggattaga taaatgcctg ctctttactg aaggctcttt actattgctt
     3841 tatgataatg tttcatagtt ggatatcata atttaaacaa gcaaaaccaa attaagggcc
     3901 agctcattcc tcccactcat gatctataga tctatagatc tctcgtggga tcattgtttt
     3961 tctcttgatt cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata
     4021 gcctgaagaa cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt
     4081 gccaagttct aattccatca gaagctggtc gagatccgga acccttaata taacttcgta
     4141 taatgtatgc tatacgaagt tattaggtcc ctcgaagagg ttcactaggc gcgcctacaa
     4201 tacagcaagg ccagatgcac atccctggaa ggacatccat gttccgagaa gaacagataa
     4261 tccctgtatt tcaagacctc tgtgcactta tttatgaacc tgccctgctc ccacagaaca
     4321 cagcaattcc tcaggctaag ctgccggttc ttaaatccat cctgctaagt taatgttggg
     4381 tagaaagaga tacagagggg ctgttgaatt tcccacatac cctccttcca ccaagttgga
     4441 acatccttgg aaattggaag agcacaagag gagatccagg gcaaggccat tgggatattc
     4501 tgaaacttga atattttgtt ttgtgcagag ataaagacct tttccatgca ccctcataca
     4561 cagaaaccaa ttttcttttt tatactcaat catttctagc gcatggcctg gttagaggct
     4621 ggttttttct cttttccttt ggtccttcaa aggcttgtag ttttggctag tccttgttct
     4681 ttggaaatac acagtgctga ccagacagcc tccccctgtc ccctctatga cctcgccctc
     4741 cacaaatggg aaaaccagac tacttgggag caccgcctgt gaaataccaa cctgaagaca
     4801 ccgttcattc aggcaacgca caaaacagaa aatgaaggtg gaacaagcac agatgttcat
     4861 tcaaacattt cgagtagcct tccacagatt aagatacatt gatgagtttg gacaaaccac
     4921 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     4981 tgtaaccatt ataagctgca ataaacaagt ttacccacaa attttgtaat ccagaggttg
     5041 attaagcttt tacttgtaca gctcgtccat gccatgtgta atcccggcgg cggtcacgaa
     5101 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct tggactgatg
     5161 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
     5221 gtagtggtcg gcgagctgca cgctgccgtc ctcaacgttg tggcggatct tgaagttagc
     5281 cttgatgccg ttcttctgct tgtcggccgt gatatagacg ttgtggctgt tgaagttgta
     5341 ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
     5401 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt aggtaccgtc
     5461 gtccttgaaa gagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
     5521 gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcag gccacgccgt agccgaaggt
     5581 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
     5641 cagcttgccg ttggtggcat cgccctcgcc ctcgccgcgg acgctgaact tgtggccgtt
     5701 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
     5761 gctcaccatg gtggcaagct ttcacgacac ctgaaatgga agaaaaaaac tttgaaccac
     5821 tgtctgaggc ttgagaatga accaagatcc aaactcaaaa agggcaaatt ccaaggagaa
     5881 ttacatcaag tgccaagctg gcctaacttc agtctccacc cactcagtgt ggggaaactc
     5941 catcgcataa aacccctccc cccaacctaa agacgacgta ctccaaaagc tcgagaacta
     6001 atcgaggtgc ctggacggcg cccggtactc cgtggagtca catgaagcga cggctgagga
     6061 cggaaaggcc cttttccttt gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt
     6121 cctccatttt gagctccctg cagcagggcc gggaagcggc catctttccg ctcacgcaac
     6181 tggtgccgac cgggccagcc ttgccgccca gggcggggcg atacacggcg gcgcgagacc
     6241 aggcaccaga gcaggccggc cagcttgaga ctacccccgt ccgattctcg gtggccgcgc
     6301 tcgcaggccc cgcctcgccg aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc
     6361 ggccccaaaa accgaaatac cagtgtgcag atcttggccc gcatttacaa gactatcttg
     6421 ccagaaaaaa agcgtcgcag caggtcatca aaaattttaa atggctagag acttatcgaa
     6481 agcagcgaga caggcgcgaa ggtgccacca gattcgcacg cggcggcccc agcgcccagg
     6541 ccaggcctca actcaagcac gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc
     6601 ccacccactt ccaacccgaa gctcgggatc aagaatcacg tactgcagcc aggtggaagt
     6661 aattcaaggc acgcaagggc cataacccgt aaagaggcca ggcccgcggg aaccacacac
     6721 ggcacttacc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca cggcgactac
     6781 tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt acacgacatc
     6841 actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc cgacccctcc
     6901 ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg caccggagcc
     6961 gcggccgcgt agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     7021 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     7081 atcccccttt cgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac
     7141 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag
     7201 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     7261 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7321 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7381 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7441 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7501 ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc cgagaaagta
     7561 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     7621 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     7681 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     7741 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     7801 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     7861 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     7921 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     7981 atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag agtatgagta
     8041 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8101 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8161 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8221 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8281 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8341 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8401 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8461 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8521 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8581 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8641 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8701 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8761 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8821 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     8881 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     8941 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9001 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9181 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9721 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9781 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     9841 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     9901 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     9961 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10021 gcggataaca atttcaca
//
