LOCUS       sequence-476203-        3886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Khan UW, Newmark PA
  TITLE     Somatic regulation of female germ cell regeneration and development 
            in planarians.
  JOURNAL   Cell Rep. 2022 Mar 15;38(11):110525. doi: 
            10.1016/j.celrep.2022.110525.
  PUBMED    35294875
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-03-15"; volume: "38(11):110525. doi"; pages: " 
            10.1016/j.celrep.2022.110525"
COMMENT     Sequence Label: pJC-lecg
FEATURES             Location/Qualifiers
     source          1..3886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..49)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        85..103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(766..784)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(767..784)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(788..806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      820..867
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(879..896)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1050..1638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1130..1149)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1809..2600,2601..2669))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2432..2451
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2687..3481)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2798..2817)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3408..3427
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagg cgcgccggat
       61 cctaatacga ctcactatag ggagaattaa ccctcactaa agggagacct ttttccgagc
      121 atggtaacat ttatttctaa acaatgtttg ttacttcaat ttccgtttta gtcgcagtag
      181 ctttcggttt cggatcctca aattccataa atgcatctat gaaaatcagt gaaggacata
      241 ttagtgacaa aatgtttgct ttcattcatg gtggagaaga cgcaagaatg aactttactg
      301 atgctgaaga gttctgttca aattttactt tccatcgaaa caacagtgac tcccatcttg
      361 cttcaattca aaatttgaag gaagtgaaac tcattcaaaa ctggatttta aacttagaaa
      421 gaaaatcctt ctggattggc ggtgaaataa tttgctcaat gaatgcacta ttagaaaaaa
      481 tgtttctatt acattggatc aataatgaca aagtggagtt cacaaatttc aaagttccca
      541 attacaatgt taaaaacatg aaagtaacag aaaaacgatg cgtttcggtt gacctccaag
      601 atggaagatg gggagttcat cattgctcag aaaaaaaata ttttctttgc ttgacaaaaa
      661 atcaattagt cactgaaaag gatgttgttg ttgataacaa ttttattact tcatcttctg
      721 ttccaaaaac cacgcccatg ttggctaaac ttgtttaagg tcacttctat agtgtcacct
      781 aaatctccct atagtgagtc gtattaggat ccggcgcgcc tagcataacc ccttggggcc
      841 tctaaacggg tcttgagggg ttttttgacg cgtgcttcct cgctcactga ctcgctgcgc
      901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1861 tcatccatag ttgcctggct ccccgtcgtg tagataacta cgatacggga gggcttacca
     1921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     1981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaattcag aagaactcgt
     2701 caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga
     2761 ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta
     2821 tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc
     2881 cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc
     2941 cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct
     3001 cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga
     3061 tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc
     3121 gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgggat gacaggagat
     3181 cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga
     3241 gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct
     3301 gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg
     3361 ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc
     3421 cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca
     3481 tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatca
     3541 ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg
     3601 ccccagctgg caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc
     3661 atgtaagccc actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga
     3721 tagcccagta gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg
     3781 tgttccgctt cctttagcag cccttgcgcc ctgaattttg ttaaaattcg cgttaaattt
     3841 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc gacgcg
//
