LOCUS       sequence-476228-        9248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9248)
  TITLE     TLR Signaling Plasmids
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-TLR7-YFP
FEATURES             Location/Qualifiers
     source          1..9248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2552..2755
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2800..2819
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2800..2818
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(6003..6005,6006..6008,6009..6722)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6048..6069)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6309..6328)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6656..6677
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        complement(6738..6756)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6739..6756)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6776..6793)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6782..7006
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7052..7480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7139..7158)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7349..7370
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7489..7509)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7494..7823
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7674..7809
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7736..7755
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7890..8684
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7944..7963)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8554..8573
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8858..8979
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8895..8914)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8949..8968
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9028..9044)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9028..9044)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9041..9063)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9052..9068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9076..9082,9083..9100,9101..9106))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9121..9142
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     2761 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
     2821 gacccaagct tggtaccgag ctcggatcca tggtgtttcc aatgtggaca ctgaagagac
     2881 aaattcttat cctttttaac ataatcctaa tttccaaact ccttggggct agatggtttc
     2941 ctaaaactct gccctgtgat gtcactctgg atgttccaaa gaaccatgtg atcgtggact
     3001 gcacagacaa gcatttgaca gaaattcctg gaggtattcc cacgaacacc acgaacctca
     3061 ccctcaccat taaccacata ccagacatct ccccagcgtc ctttcacaga ctggaccatc
     3121 tggtagagat cgatttcaga tgcaactgtg tacctattcc actggggtca aaaaacaaca
     3181 tgtgcatcaa gaggctgcag attaaaccca gaagctttag tggactcact tatttaaaat
     3241 ccctttacct ggatggaaac cagctactag agataccgca gggcctcccg cctagcttac
     3301 agcttctcag ccttgaggcc aacaacatct tttccatcag aaaagagaat ctaacagaac
     3361 tggccaacat agaaatactc tacctgggcc aaaactgtta ttatcgaaat ccttgttatg
     3421 tttcatattc aatagagaaa gatgccttcc taaacttgac aaagttaaaa gtgctctccc
     3481 tgaaagataa caatgtcaca gccgtcccta ctgttttgcc atctacttta acagaactat
     3541 atctctacaa caacatgatt gcaaaaatcc aagaagatga ttttaataac ctcaaccaat
     3601 tacaaattct tgacctaagt ggaaattgcc ctcgttgtta taatgcccca tttccttgtg
     3661 cgccgtgtaa aaataattct cccctacaga tccctgtaaa tgcttttgat gcgctgacag
     3721 aattaaaagt tttacgtcta cacagtaact ctcttcagca tgtgccccca agatggttta
     3781 agaacatcaa caaactccag gaactggatc tgtcccaaaa cttcttggcc aaagaaattg
     3841 gggatgctaa atttctgcat tttctcccca gcctcatcca attggatctg tctttcaatt
     3901 ttgaacttca ggtctatcgt gcatctatga atctatcaca agcattttct tcactgaaaa
     3961 gcctgaaaat tctgcggatc agaggatatg tctttaaaga gttgaaaagc tttaacctct
     4021 cgccattaca taatcttcaa aatcttgaag ttcttgatct tggcactaac tttataaaaa
     4081 ttgctaacct cagcatgttt aaacaattta aaagactgaa agtcatagat ctttcagtga
     4141 ataaaatatc accttcagga gattcaagtg aagttggctt ctgctcaaat gccagaactt
     4201 ctgtagaaag ttatgaaccc caggtcctgg aacaattaca ttatttcaga tatgataagt
     4261 atgcaaggag ttgcagattc aaaaacaaag aggcttcttt catgtctgtt aatgaaagct
     4321 gctacaagta tgggcagacc ttggatctaa gtaaaaatag tatatttttt gtcaagtcct
     4381 ctgattttca gcatctttct ttcctcaaat gcctgaatct gtcaggaaat ctcattagcc
     4441 aaactcttaa tggcagtgaa ttccaacctt tagcagagct gagatatttg gacttctcca
     4501 acaaccggct tgatttactc cattcaacag catttgaaga gcttcacaaa ctggaagttc
     4561 tggatataag cagtaatagc cattattttc aatcagaagg aattactcat atgctaaact
     4621 ttaccaagaa cctaaaggtt ctgcagaaac tgatgatgaa cgacaatgac atctcttcct
     4681 ccaccagcag gaccatggag agtgagtctc ttagaactct ggaattcaga ggaaatcact
     4741 tagatgtttt atggagagaa ggtgataaca gatacttaca attattcaag aatctgctaa
     4801 aattagagga attagacatc tctaaaaatt ccctaagttt cttgccttct ggagtttttg
     4861 atggtatgcc tccaaatcta aagaatctct ctttggccaa aaatgggctc aaatctttca
     4921 gttggaagaa actccagtgt ctaaagaacc tggaaacttt ggacctcagc cacaaccaac
     4981 tgaccactgt ccctgagaga ttatccaact gttccagaag cctcaagaat ctgattctta
     5041 agaataatca aatcaggagt ctgacgaagt attttctaca agatgccttc cagttgcgat
     5101 atctggatct cagctcaaat aaaatccaga tgatccaaaa gaccagcttc ccagaaaatg
     5161 tcctcaacaa tctgaagatg ttgcttttgc atcataatcg gtttctgtgc acctgtgatg
     5221 ctgtgtggtt tgtctggtgg gttaaccata cggaggtgac tattccttac ctggccacag
     5281 atgtgacttg tgtggggcca ggagcacaca agggccaaag tgtgatctcc ctggatctgt
     5341 acacctgtga gttagatctg actaacctga ttctgttctc actttccata tctgtatctc
     5401 tctttctcat ggtgatgatg acagcaagtc acctctattt ctgggatgtg tggtatattt
     5461 accatttctg taaggccaag ataaaggggt atcagcgtct aatatcacca gactgttgct
     5521 atgatgcttt tattgtgtat gacactaaag acccagctgt gaccgagtgg gttttggctg
     5581 agctggtggc caaactggaa gacccaagag agaaacattt taatttatgt ctcgaggaaa
     5641 gggactggtt accagggcag ccagttctgg aaaacctttc ccagagcata cagcttagca
     5701 aaaagacagt gtttgtgatg acagacaagt atgcaaagac tgaaaatttt aagatagcat
     5761 tttacttgtc ccatcagagg ctcatggatg aaaaagttga tgtgattatc ttgatatttc
     5821 ttgagaagcc ctttcagaag tccaagttcc tccagctccg gaaaaggctc tgtgggagtt
     5881 ctgtccttga gtggccaaca aacccgcaag ctcacccata cttctggcag tgtctaaaga
     5941 acgccctggc cacagacaat catgtggcct atagtcaggt gttcaaggaa acggtcgtcg
     6001 agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     6061 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     6121 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6181 ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc gaccacatga
     6241 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6301 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6361 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6421 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6481 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6541 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6601 actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6661 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6721 aatctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     6781 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6841 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6901 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6961 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7021 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggagc attaagcgcg
     7081 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     7141 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     7201 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     7261 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     7321 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     7381 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     7441 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     7501 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7561 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7621 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     7681 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     7741 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     7801 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     7861 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     7921 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     7981 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8041 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     8101 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     8161 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     8221 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     8281 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     8341 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     8401 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     8461 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     8521 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     8581 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     8641 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     8701 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     8761 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     8821 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     8881 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     8941 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     9001 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     9061 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     9121 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9181 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9241 tgggcgct
//
