LOCUS       sequence-476230-        3481 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  riboprobes (SP6 or T3) and dsRNA (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Rozario T, Quinn EB, Wang J, Davis RE, Newmark PA
  TITLE     Region-specific regulation of stem cell-driven regeneration in 
            tapeworms.
  JOURNAL   eLife. 2019 Sep 24;8. pii: 48958. doi: 10.7554/eLife.48958.
  PUBMED    31549962
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2019-09-24"; volume: "8. pii: 48958. doi"; pages: " 
            10.7554/eLife.48958"
COMMENT     Sequence Label: TR1_P1B08_HDp_00300
FEATURES             Location/Qualifiers
     source          1..3481
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2..49)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        85..103
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(361..379)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(362..379)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(383..401)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      415..462
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(474..491)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(645..1233)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(725..744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1404..2195,2196..2264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2027..2046
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(2282..3076)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2393..2412)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3003..3022
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctagg cgcgccggat
       61 cctaatacga ctcactatag ggagaattaa ccctcactaa agggagacct tatgcgcata
      121 ctctgtacca tagacaagtt tcagaacaac aatcgctatc gttccagcaa aatctgcctt
      181 tgccgagaca aactgcacat ttgccgttga aaagaccagt caattcacag cgtaaattac
      241 cacagcagcg attgaatacc gttccatcac accattctcc cagatcttga caatgttctt
      301 gtgatttgac agtggcaaat atgcaggcca aaagagcgaa aatgacagca caaggtcact
      361 tctatagtgt cacctaaatc tccctatagt gagtcgtatt aggatccggc gcgcctagca
      421 taaccccttg gggcctctaa acgggtcttg aggggttttt tgacgcgtgc ttcctcgctc
      481 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
      541 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
      601 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      661 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      721 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      781 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      841 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      901 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      961 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1021 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1081 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1141 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1201 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1261 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     1321 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     1381 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     1441 atctgtctat ttcgttcatc catagttgcc tggctccccg tcgtgtagat aactacgata
     1501 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     1561 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     1621 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     1681 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     1741 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     1801 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     1861 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     1921 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     1981 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     2041 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     2101 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     2161 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     2221 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     2281 ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga
     2341 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac
     2401 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga
     2461 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca
     2521 cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg
     2581 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag
     2641 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa
     2701 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt
     2761 gggatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt
     2821 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc
     2881 gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa
     2941 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt
     3001 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc
     3061 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc
     3121 tgcgccatca gatcattggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa
     3181 ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc
     3241 agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt
     3301 tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg
     3361 gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgaa ttttgttaaa
     3421 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcgacgc
     3481 g
//
