LOCUS       sequence-476238-       12689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible TGF beta 3 and mCherry; constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12689)
  AUTHORS   Walton BL, Shattuck-Brandt R, Hamann CA, Tung VW, Colazo JM, Brand 
            DD, Hasty KA, Duvall CL, Brunger JM
  TITLE     A programmable arthritis-specific receptor for guided articular 
            cartilage regenerative medicine.
  JOURNAL   Osteoarthritis Cartilage. 2025 Feb;33(2):231-240. doi: 
            10.1016/j.joca.2024.12.002. Epub 2024 Dec 18.
  PUBMED    39706287
REFERENCE   2  (bases 1 to 12689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.joca.2024.12.002"; journalName: "Osteoarthritis 
            Cartilage"; date: "2025-02"; volume: "33"; issue: "2"; pages: 
            "231-240"
COMMENT     Sequence Label: pHR TRE_TGFbeta3_IRES_mCherry-PGK_BFP
FEATURES             Location/Qualifiers
     source          1..12689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             82..315
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(340..358)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(341..358)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(648..667)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(881..900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1060..1078)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1146..1250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1251..1319,1320..2111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1469..1488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2282..2870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2771..2790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3024..3041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3111..3131)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3116..3445
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3296..3431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4579..4644
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4774..4794
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5219..5353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5256..5275)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5310..5329
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5521..6154
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6201..6326
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6823..7056
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7241..7285
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7570..7687
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7766..8133
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    7774..7792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7810..7828
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7846..7864
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7882..7900
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7918..7936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7954..7972
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    7990..8008
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    9425..9998
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9592..9609)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9819..9838
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10017..10727
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10162..10180)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10430..10449
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        10756..11255
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10791..10814)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     11188..11207
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             11276..11977
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    12073..12661
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(12126..12146)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(12544..12555)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12664..12680)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12665..12681)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
       61 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
      121 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      181 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      241 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      301 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
      361 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
      421 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
      481 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
      541 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
      601 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
      661 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
      721 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
      781 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
      841 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
      901 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1081 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1261 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1321 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1381 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1441 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1501 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1561 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1621 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1681 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1741 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1801 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1861 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1921 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1981 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2041 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2101 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2161 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2221 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2281 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2341 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2401 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2461 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2521 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2581 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2641 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2701 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2761 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2821 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2881 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2941 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3001 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     3061 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     3121 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3181 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3241 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3301 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3361 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3421 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     3481 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     3541 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     3601 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     3661 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     3721 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     3781 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     3841 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     3901 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     3961 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     4021 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     4081 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     4141 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     4201 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     4261 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     4321 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     4381 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     4441 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     4501 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     4561 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     4621 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     4681 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     4741 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     4801 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     4861 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     4921 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     4981 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     5041 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     5101 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     5161 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     5221 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5281 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5341 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     5401 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     5461 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     5521 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     5581 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     5641 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     5701 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5761 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     5821 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     5881 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     5941 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     6001 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6061 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6121 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     6181 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     6241 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     6301 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     6361 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     6421 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     6481 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     6541 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     6601 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     6661 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     6721 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     6781 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     6841 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     6901 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     6961 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     7021 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     7081 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     7141 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     7201 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     7261 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     7321 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     7381 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     7441 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     7501 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     7561 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     7621 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     7681 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     7741 cgaattcccg tggaattatc acctcgagtt tactccctat cagtgataga gaacgtatga
     7801 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     7861 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     7921 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     7981 ccagtttact ccctatcagt gatagagaac gtataagctt tgcttatgta aaccagggcg
     8041 cctataaaag agtgctgatt ttttgagtaa acttcaattc cacaacactt ttgtcttata
     8101 ccaactttcc gtaccacttc ctaccctcgt aaagaattcc cgtatacacc ggtgcaattc
     8161 tcacgcgtgc cgccaccatg aagatgcact tgcaaagggc tctggtggtc ctggccctgc
     8221 tgaactttgc cacggtcagc ctctctctgt ccacttgcac caccttggac ttcggccaca
     8281 tcaagaagaa gagggtggaa gccattaggg gacagatctt gagcaagctc aggctcacca
     8341 gcccccctga gccaacggtg atgacccacg tcccctatca ggtcctggcc ctttacaaca
     8401 gcacccggga gctgctggag gagatgcatg gggagaggga ggaaggctgc acccaggaaa
     8461 acaccgagtc ggaatactat gccaaagaaa tccataaatt cgacatgatc caggggctgg
     8521 cggagcacaa cgaactggct gtctgcccta aaggaattac ctccaaggtt ttccgcttca
     8581 atgtgtcctc agtggagaaa aatagaacca acctattccg agcagaattc cgggtcttgc
     8641 gggtgcccaa ccccagctct aagcggaatg agcagaggat cgagctcttc cagatccttc
     8701 ggccagatga gcacattgcc aaacagcgct atatcggtgg caagaatctg cccacacggg
     8761 gcactgccga gtggctgtcc tttgatgtca ctgacactgt gcgtgagtgg ctgttgagaa
     8821 gagagtccaa cttaggtcta gaaatcagca ttcactgtcc atgtcacacc tttcagccca
     8881 atggagatat cctggaaaac attcacgagg tgatggaaat caaattcaaa ggcgtggaca
     8941 atgaggatga ccatggccgt ggagatctgg ggcgcctcaa gaagcagaag gatcaccaca
     9001 accctcatct aatcctcatg atgattcccc cacaccggct cgacaacccg ggccaggggg
     9061 gtcagaggaa gaagcgggct ttggacacca attactgctt ccgcaacttg gaggagaact
     9121 gctgtgtgcg ccccctctac attgacttcc gacaggatct gggctggaag tgggtccatg
     9181 aacctaaggg ctactatgcc aacttctgct caggcccttg cccatacctc cgcagtgcag
     9241 acacaaccca cagcacggtg ctgggactgt acaacactct gaaccctgaa gcatctgcct
     9301 cgccttgctg cgtgccccag gacctggagc ccctgaccat cctgtactat gttgggagga
     9361 cccccaaagt ggagcagctc tccaacatgg tggtgaagtc ttgtaaatgt agctgagcgg
     9421 ccgccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     9481 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     9541 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     9601 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     9661 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     9721 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     9781 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     9841 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     9901 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     9961 acggggacgt ggttttcctt tgaaaaacac gatgataaat gagcgctggc gggtccatgg
    10021 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
    10081 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
    10141 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
    10201 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
    10261 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
    10321 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
    10381 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
    10441 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
    10501 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
    10561 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
    10621 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
    10681 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagggc cgcctcgagg
    10741 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
    10801 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
    10861 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
    10921 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
    10981 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
    11041 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
    11101 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
    11161 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
    11221 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatgag
    11281 cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
    11341 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
    11401 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
    11461 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
    11521 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
    11581 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
    11641 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
    11701 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
    11761 gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa
    11821 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
    11881 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
    11941 cgacctccct agcaaactgg ggcacaagct taattaaggc cgcaactccc acctgcaaca
    12001 tgcgtgactg actgaggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc
    12061 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
    12121 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
    12181 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
    12241 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
    12301 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
    12361 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
    12421 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
    12481 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
    12541 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
    12601 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
    12661 catcgatacc gtcgacctcg agggaatta
//
