LOCUS       sequence-476239-       10672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible IL-1Ra; constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Walton BL, Shattuck-Brandt R, Hamann CA, Tung VW, Colazo JM, Brand 
            DD, Hasty KA, Duvall CL, Brunger JM
  TITLE     A programmable arthritis-specific receptor for guided articular 
            cartilage regenerative medicine.
  JOURNAL   Osteoarthritis Cartilage. 2025 Feb;33(2):231-240. doi: 
            10.1016/j.joca.2024.12.002. Epub 2024 Dec 18.
  PUBMED    39706287
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.joca.2024.12.002"; journalName: "Osteoarthritis 
            Cartilage"; date: "2025-02"; volume: "33"; issue: "2"; pages: 
            "231-240"
COMMENT     Sequence Label: pHR TRE_IL1Ra-PGK_BFP
FEATURES             Location/Qualifiers
     source          1..10672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        224..591
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    268..286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    304..322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    340..358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    412..430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    448..466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      627..636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1197..1696
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1232..1255)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1629..1648
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1717..2418
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2514..3102
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2567..2587)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2985..2996)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3105..3121)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3106..3122)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3212..3445
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3470..3488)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3471..3488)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3778..3797)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4011..4030)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4130..4152
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4190..4208)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4276..4380
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4381..4449,4450..5241)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4599..4618)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5412..6000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5901..5920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6154..6171
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6241..6261)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6246..6575
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6426..6561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7709..7774
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7904..7924
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8349..8483
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8386..8405)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8440..8459
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8651..9284
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9331..9456
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9953..10186
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10371..10415
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
       61 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      121 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
      181 gatcgataag cttgatatcg aattcccgtg gaattatcac ctcgagttta ctccctatca
      241 gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt
      301 tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac
      361 gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca
      421 gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt ataagctttg
      481 cttatgtaaa ccagggcgcc tataaaagag tgctgatttt ttgagtaaac ttcaattcca
      541 caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa agaattcccg
      601 tatacaccgg tgcaattctc acgcgtgcca ccatggaaat ctgcagaggc ctccgcagtc
      661 acctaatcac tctcctcctc ttcctgttcc attcagagac gatctgccga ccctctggga
      721 gaaaatccag caagatgcaa gccttcagaa tctgggatgt taaccagaag accttctatc
      781 tgaggaacaa ccaactagtt gctggatact tgcaaggacc aaatgtcaat ttagaagaaa
      841 agatagatgt ggtacccatt gagcctcatg ctctgttctt gggaatccat ggagggaaga
      901 tgtgcctgtc ctgtgtcaag tctggtgatg agaccagact ccagctggag gcagttaaca
      961 tcactgacct gagcgagaac agaaagcagg acaagcgctt cgccttcatc cgctcagaca
     1021 gtggccccac caccagtttt gagtctgccg cctgccccgg ttggttcctc tgcacagcga
     1081 tggaagctga ccagcccgtc agcctcacca atatgcctga cgaaggcgtc atggtcacca
     1141 aattctactt ccaggaggac gagtaagcgg ccgcctcgag gttaacgaat tctaccgggt
     1201 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     1261 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     1321 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     1381 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     1441 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     1501 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     1561 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     1621 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     1681 atctccgggc ctttcgacct gcagcccaag cttaccatga gcgagctgat taaggagaac
     1741 atgcacatga agctgtacat ggagggcacc gtggacaacc atcacttcaa gtgcacatcc
     1801 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc
     1861 ggccctctcc ccttcgcctt cgacatcctg gctactagct tcctctacgg cagcaagacc
     1921 ttcatcaacc acacccaggg catccccgac ttcttcaagc agtccttccc tgagggcttc
     1981 acatgggaga gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc
     2041 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcacatcc
     2101 aacggccctg tgatgcagaa gaaaacactc ggctgggagg ccttcaccga gacgctgtac
     2161 cccgctgacg gcggcctgga aggcagaaac gacatggccc tgaagctcgt gggcgggagc
     2221 catctgatcg caaacatcaa gaccacatat agatccaaga aacccgctaa gaacctcaag
     2281 atgcctggcg tctactatgt ggactacaga ctggaaagaa tcaaggaggc caacaacgag
     2341 acctacgtcg agcagcacga ggtggcagtg gccagatact gcgacctccc tagcaaactg
     2401 gggcacaagc ttaattaagg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     2461 gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac
     2521 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2581 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2641 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2701 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2761 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2821 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2881 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2941 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3001 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3061 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     3121 gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     3181 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     3241 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     3301 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3361 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3421 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
     3481 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     3541 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     3601 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     3661 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
     3721 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     3781 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     3841 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     3901 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     3961 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     4021 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4081 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4141 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4201 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4261 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4501 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4561 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4621 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4681 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4741 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4801 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4921 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5101 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5161 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5221 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5521 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6061 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6121 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6181 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     6241 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6301 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6361 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6421 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6481 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6541 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     6601 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     6661 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     6721 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     6781 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     6841 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     6901 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     6961 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     7021 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     7081 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     7141 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     7201 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     7261 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     7321 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     7381 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     7441 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     7501 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     7561 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     7621 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     7681 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     7741 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     7801 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     7861 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     7921 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     7981 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     8041 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     8101 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     8161 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     8221 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     8281 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8341 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8401 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8461 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     8521 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     8581 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     8641 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     8701 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     8761 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     8821 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     8881 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     8941 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     9001 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     9061 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     9121 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9181 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9241 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     9301 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     9361 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     9421 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9481 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9541 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9601 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9661 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9721 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9781 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9841 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9901 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9961 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
    10021 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10081 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10141 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10201 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10261 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10321 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10381 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10441 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10501 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10561 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10621 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg ta
//
