LOCUS       sequence-476240-       12565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible firefly luciferase and mCherry; constitutive BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Walton BL, Shattuck-Brandt R, Hamann CA, Tung VW, Colazo JM, Brand 
            DD, Hasty KA, Duvall CL, Brunger JM
  TITLE     A programmable arthritis-specific receptor for guided articular 
            cartilage regenerative medicine.
  JOURNAL   Osteoarthritis Cartilage. 2025 Feb;33(2):231-240. doi: 
            10.1016/j.joca.2024.12.002. Epub 2024 Dec 18.
  PUBMED    39706287
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.joca.2024.12.002"; journalName: "Osteoarthritis 
            Cartilage"; date: "2025-02"; volume: "33"; issue: "2"; pages: 
            "231-240"
COMMENT     Sequence Label: pHR TRE_firefly Luciferase_2A_mCherry-PGK_BFP
FEATURES             Location/Qualifiers
     source          1..12565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..145
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        224..591
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    232..250
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    268..286
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    304..322
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    340..358
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    376..394
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    412..430
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    448..466
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2289..2345
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2349..3059
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2494..2512)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2762..2781
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        3090..3589
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3125..3148)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3522..3541
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3610..4311
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4407..4995
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4460..4480)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4878..4889)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4998..5014)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4999..5015)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5105..5338
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5363..5381)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5364..5381)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5671..5690)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5904..5923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6023..6045
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6083..6101)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6169..6273
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6274..6342,6343..7134)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6492..6511)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7305..7893
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7794..7813
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8047..8064
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8134..8154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8139..8468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8319..8454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9602..9667
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9797..9817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10242..10376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10279..10298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10333..10352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10544..11177
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11224..11349
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11846..12079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12264..12308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
       61 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      121 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
      181 gatcgataag cttgatatcg aattcccgtg gaattatcac ctcgagttta ctccctatca
      241 gtgatagaga acgtatgaag agtttactcc ctatcagtga tagagaacgt atgcagactt
      301 tactccctat cagtgataga gaacgtataa ggagtttact ccctatcagt gatagagaac
      361 gtatgaccag tttactccct atcagtgata gagaacgtat ctacagttta ctccctatca
      421 gtgatagaga acgtatatcc agtttactcc ctatcagtga tagagaacgt ataagctttg
      481 cttatgtaaa ccagggcgcc tataaaagag tgctgatttt ttgagtaaac ttcaattcca
      541 caacactttt gtcttatacc aactttccgt accacttcct accctcgtaa agaattcccg
      601 tatacaccgg tgcaattctc acgcgtgccg ccaccatgga agatgccaag aacatcaaga
      661 aaggccctgc ccccttctac cccctggaag atggcacagc cggcgagcag ctgcacaagg
      721 ccatgaagag atacgccctg gtgcccggca ccatcgcctt caccgacgcc cacatcgagg
      781 tggacatcac ctacgccgag tatttcgaga tgagcgtgcg gctggccgag gccatgaaac
      841 gctacggcct gaacaccaac caccggatcg tggtgtgcag cgagaacagc ctgcagttct
      901 tcatgcccgt gctgggcgcc ctgttcatcg gcgtggccgt ggcccctgcc aacgacatct
      961 acaacgagcg ggagctgctg aacagcatgg gcatcagcca gcccaccgtg gtgttcgtga
     1021 gcaagaaggg cctgcagaaa atcctgaacg tgcagaagaa gctgcccatc atccagaaaa
     1081 tcatcatcat ggacagcaag accgactacc agggcttcca gagcatgtac accttcgtga
     1141 ccagccacct gccccctggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
     1201 acaagaccat cgccctgatc atgaacagca gcggcagcac cggcctgcct aaaggcgtgg
     1261 ccctgcctca ccggaccgcc tgcgtgcggt tcagccacgc ccgggacccc atcttcggca
     1321 accagatcat ccccgacacc gccatcctga gcgtggtgcc cttccaccac ggcttcggca
     1381 tgttcaccac cctgggctac ctgatctgcg gcttccgggt ggtgctgatg taccggttcg
     1441 aggaagagct gttcctgcgg agcctgcagg actacaagat ccagagcgcc ctgctggtgc
     1501 ccaccctgtt cagctttttc gccaagagca ccctgatcga caagtacgac ctgagcaacc
     1561 tgcacgagat cgccagcggc ggagcccccc tgtccaaaga agtgggcgaa gccgtcgcca
     1621 agcggttcca cctgcccggc atccggcagg gctatggcct gaccgagacc acaagcgcca
     1681 ttctgatcac ccccgagggc gacgacaagc ctggcgccgt gggcaaggtg gtgcctttct
     1741 tcgaggccaa ggtggtggac ctggacaccg gcaagaccct gggcgtgaac cagcggggcg
     1801 agctgtgcgt gaggggcccc atgatcatga gcggctacgt gaacaacccc gaggccacca
     1861 acgccctgat tgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     1921 acgagcactt cttcatcgtg gaccggctga agagcctgat caagtacaag ggctaccagg
     1981 tggccccagc cgagctggaa agcatcctgc tgcagcaccc caacatcttc gatgccgggg
     2041 tggccggact gcccgacgac gatgccggcg agctgcctgc cgccgtggtg gtgctggaac
     2101 acggcaaaac catgaccgag aaagaaatcg tggactacgt ggccagccag gtgaccaccg
     2161 ccaagaaact gagaggcggc gtggtgtttg tggacgaggt gcccaagggc ctgacaggca
     2221 agctggacgc ccggaagatc cgggagatcc tgatcaaggc caagaagggc ggcaagagag
     2281 ccaagagagc cacaaacttc tctctgctaa agcaagcagg tgatgttgaa gaaaaccccg
     2341 ggcctcatat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     2401 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     2461 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     2521 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     2581 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     2641 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2701 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2761 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2821 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2881 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2941 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     3001 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag
     3061 cggccgcctc gaggttaacg aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     3121 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     3181 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     3241 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     3301 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     3361 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     3421 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     3481 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     3541 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     3601 aagcttacca tgagcgagct gattaaggag aacatgcaca tgaagctgta catggagggc
     3661 accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc
     3721 acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc
     3781 ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca gggcatcccc
     3841 gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac cacatacgaa
     3901 gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg cctcatctac
     3961 aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca
     4021 ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct ggaaggcaga
     4081 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
     4141 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
     4201 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
     4261 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatta aggccgcaac
     4321 tcccacctgc aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat
     4381 gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     4441 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4501 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4561 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4621 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4681 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4741 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4801 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4861 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4921 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4981 gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct
     5041 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5101 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5161 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5221 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5281 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     5341 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     5401 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     5461 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     5521 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     5581 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     5641 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     5701 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     5761 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     5821 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     5881 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5941 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     6001 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     6061 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     6121 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6181 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6241 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6301 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6361 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6421 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6481 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6541 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6601 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6661 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6721 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6781 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6841 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6901 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6961 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7021 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7081 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7141 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7201 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7261 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7321 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7381 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7441 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7501 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7561 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7621 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7681 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7741 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7801 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7861 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7921 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7981 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8041 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     8101 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     8161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     8281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     8341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     8401 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     8461 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     8521 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     8581 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     8641 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     8701 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     8761 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     8821 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     8881 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     8941 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     9001 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     9061 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     9121 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     9181 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     9241 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     9301 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     9361 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     9421 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     9481 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     9541 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     9601 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     9661 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     9721 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     9781 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     9841 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     9901 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     9961 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
    10021 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
    10081 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
    10141 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    10201 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    10261 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10321 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10381 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10441 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10501 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    10561 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10621 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    10681 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    10741 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    10801 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    10861 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    10921 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    10981 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    11161 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    11221 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    11281 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    11341 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    11401 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    11461 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    11521 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    11581 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    11641 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga
    11701 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
    11761 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
    11821 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
    11881 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
    11941 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
    12001 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
    12061 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
    12121 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
    12181 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
    12241 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    12301 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    12361 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    12421 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    12481 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
    12541 gattagtgaa cggatctcga cggta
//
