LOCUS       sequence-476274-       14229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  induction of phase-separated condensate mediated by FUSN-IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Kim YJ, Lee M Jr, Lee YT, Jing J, Sanders JT, Botten GA, He L, Lyu 
            J, Zhang Y, Mettlen M, Ly P, Zhou Y, Xu J
  TITLE     Light-activated macromolecular phase separation modulates 
            transcription by reconfiguring chromatin interactions.
  JOURNAL   Sci Adv. 2023 Mar 31;9(13):eadg1123. doi: 10.1126/sciadv.adg1123. 
            Epub 2023 Mar 31.
  PUBMED    37000871
REFERENCE   2  (bases 1 to 14229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03-31"; volume: "9(13):eadg1123. doi"; pages: " 
            10.1126/sciadv.adg1123. Epub 2023 Mar 31"
COMMENT     Sequence Label: dCas9-CRY2Clust-FUSN
FEATURES             Location/Qualifiers
     source          1..14229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(108..127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      921..1509
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1410..1429
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1663..1680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1797..1818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1833..1838,1839..1856,1857..1863)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1871..1887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1876..1898
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1895..1911
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1895..1911
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2039..2672
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2879..3467
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2985..2996
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3394..3414
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3720..3740)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(3750..5333,5334..5336,5337..7823,7824..7826,
                     7827..7850))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(7851..7871)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7902..7946)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7979..7996
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(9576..10283)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9851..9870)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     10120..10138
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(10555..10566)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(10975..11178)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(11004..11024)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        11179..11482
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    11522..11637
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(11900..11944)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12129..12362
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12859..12984
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             13031..13664
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    13832..13966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13856..13875)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13910..13929
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        14014..14118
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(14119..14187,14188..14229,1..750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
       61 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      121 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      181 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      241 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      301 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      361 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      421 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      481 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      541 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      601 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      661 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
      721 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
      781 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
      841 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
      901 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
      961 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1021 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1081 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1141 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1201 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1261 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1321 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1381 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1441 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1501 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1561 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1621 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1681 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1741 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1801 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     1861 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     1921 gccaagctct agctagaggt cgacggtaaa cgctagcaat gtcaaggcct ctcactctct
     1981 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactactg
     2041 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     2101 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     2161 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     2221 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     2281 gcccaggcca cgcctccctg gaaagtcccc agcggaaagt cccttgtagc aagctcgata
     2341 tcagcagttc ttgaagtact ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg
     2401 gctgtcaaac ctccactcta acacttctct ctccgggtca tccatcccat gcaggctcac
     2461 agggtgtaac aagctggtgt tctctccttt attggcctct tctaccttat ctggctcaac
     2521 tggtactagc ttgtagcacc atccaaaggt cagtggatat ctgacccctg gccctggtgt
     2581 gtagttctgc taatcaggga agtagccttg tgtgtggtag atccacagat caaggatatc
     2641 ttgtcttcgt tgggagtgaa ttagcccttc cagtcccctc ttttctttta aaaagtggct
     2701 aagatctaca gctgccttgt aagtcattgg tcttaaaggt acctgaggtg tgactggaaa
     2761 acccacctcc tcctcctctt gtgcttctag ccaggcacaa tcagcattgg tagctgctgt
     2821 attgctactt gtgattgctc catgtttttc taggtctcga ctgcagaatt aattccaggc
     2881 ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga
     2941 ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg
     3001 acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccatgga
     3061 aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct
     3121 gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa
     3181 gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg
     3241 tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa
     3301 agagacagca accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata
     3361 gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta
     3421 agaataccag tcaatctttc acaaattttg taatccagag gttgattgtt ccagacgcgc
     3481 taaacgccga tcttgattag accttgacca ccttgaacag catctctttc cttaactaac
     3541 accttttcaa cttttccgtc agtaggagcg ttaatttctg tctccatttt catagcctcc
     3601 agcaccagaa ctgtttgacc agctttaaca gtgtcacctt cttttaccag tatcttagaa
     3661 acggtaccag caagaggggc agggatttca ccttcaccgg cctttccggc ggaactacct
     3721 accttgcgct ttttcttggg agctccctca tcccctccga gctgtgagag gtcaatccga
     3781 gtttcgtaaa gtccggtgat gctctggtgg atgagggtgg cgtccagcac ttctttggtg
     3841 gaggtatagc gtttccggtc gatggtggtg tcgaagtact tgaatgcggc aggggcacca
     3901 agattggtga gggtgaacag gtggatgata ttctcagcct gctcccgaat tggtttgtct
     3961 ctgtgcttat tgtatgcgct aaggacttta tcgaggttgg catcagcgag aatgacgcgt
     4021 ttagaaaact cggaaatctg ctcaatgatc tcgtccagat aatgcttatg ctgctccaca
     4081 aacagttgct tttgctcgtt gtcctcgggg ctccccttca gcttctcgta atggcttgca
     4141 aggtagagga aattcacgta cttggagggg agagccagct cattaccctt ctgaagttcg
     4201 ccagcggagg cgagcatccg cttccgccca ttctccagtt cgaacaggct atactttggg
     4261 agcttgatga taaggtcctt cttgacttcc ttgtaacctt tggcttcgag aaagtcaatt
     4321 gggttcttct cgaaggagct gcgctccata atggtgatac ccagcagttc cttcacgctc
     4381 ttgagcttct tagactttcc tttttccacc ttagccacga ccaggacaga ataagccaca
     4441 gttggtgaat cgaatccgcc gtacttctta gggtcccaat ccttcttcct tgcaatgagc
     4501 ttgtcggagt tgcgcttggg gaggattgat tccttagaaa agcctccggt ttgcacctcg
     4561 gttttcttca cgatgttcac ttgaggcata gaaagcactt tgcgcacggt agcgaaatct
     4621 ctgcccttgt cccacacgat ttcgccagtc tccccattag tctcgatgag cggcctcttc
     4681 cggatctcac cgtttgcaag ggtgatttcg gtcttgaaga aattcatgat gttgctgtag
     4741 aagaagtact ttgcggtggc ctttccgatc tcctgctcag atttggcaat catcttgcgc
     4801 acgtcgtaga ccttgtagtc tccgtacacg aactcgcttt ccagctttgg gtacttcttg
     4861 atcagggcgg ttcccaccac ggcattcaga tatgcgtcat gagcatgatg atagttattg
     4921 atctcccgca ctttgtagaa ttgaaagtcc tttctgaaat cgctgaccag cttggacttc
     4981 agggtaatca ccttcacctc gcggataagt ttgtcgtttt cgtcgtactt ggtattcatg
     5041 cgtgagtcaa ggatttgggc gacatgttta gtaatctgcc gagtctccac aagttgccgt
     5101 ttaatgaatc ctgctttgtc cagttcgctc agtccgcccc tctcagcctt agtgagattg
     5161 tcgaacttcc gctgagtgat cagctttgcg ttcagaagct ggcgccaata gttcttcatc
     5221 tttttcacga cttcctctga gggcacgtta tctgactttc cccggttctt gtctgagcga
     5281 gtcagcacct tattgtcaat tgagtcgtct ttgaggaagc tctgagggac gatggcgtcc
     5341 acgtcgtagt ctgagagcct gttgatatca agctcttggt ccacatacat atcgcgtcca
     5401 ttttgaaggt agtacagata gagcttctca ttctgaagct gggtgttttc caccgggtgc
     5461 tctttaagga tctgtgaccc gagctcctta atcccttcct caattctttt catccgctcc
     5521 ctgctgttct tctgtccctt ctgggtagtc tggttttccc tggccatctc gatgacaatg
     5581 ttctctggtt tatggcgacc catcaccttg accagctcgt ccacgacttt cacggtctgg
     5641 agaataccct tcttaatagc gggtgaacca gccagatttg cgatatgctc gtgcagtgag
     5701 tcaccttgtc cagacacttg ggccttctgg atgtcctcct taaaggtcag ggaatcatca
     5761 tggataagtt gcatgaagtt gcgattagcg aatccgtcgc tcttaaggaa atcaagaatg
     5821 gtctttccgc tctgtttgtc ccggattccg ttgatgagtt tgcggctaag gcgtccccat
     5881 ccggtatatc ttctccgttt aagttgcttc atcactttat cgtcgaacag atgggcgtag
     5941 gtcttaagcc tctcctcgat catttcccgg tcctcgaaca gagtgagagt caggacaatg
     6001 tcctcaagga tgtcctcgtt ctcctcattg tccagaaaat ccttgtcctt gatgatctta
     6061 agcagatcat ggtaggtacc caggcttgcg ttgaaccgat cttcgacccc gctaatctcc
     6121 acagagtcga agcattcaat tttcttgaaa tagtcctcct tcagctgctt cacggtcacc
     6181 tttctattgg tcttgaacag aaggtccaca attgccttct tttgttcgcc tgacaggaat
     6241 gcgggcttcc tcatcccttc ggtgacatat ttcaccttgg tcagctcgtt gtacacggta
     6301 aagtactcgt agagcaggga atgtttcggc aggaccttct cattggggag gttcttatcg
     6361 aagttagtca tccgttcgat gaatgactga gcgctggctc ccttgtccac cacctcttcg
     6421 aagttccagg gagtgatagt ttcttctgac tttctggtca tccaagcaaa ccggctattt
     6481 cctctggcga ggggtcccac gtagtagggg atgcggaaag tgagaatttt ctcaatcttc
     6541 tccctgttgt ccttaagaaa ggggtagaag tcctcttggc gccgaaggat ggcgtgaagc
     6601 tccccaagat ggatctggtg gggaatgctc ccattgtcga aagtgcgctg cttccgcagc
     6661 agatcctccc gattcagttt cacgaggagc tcttcggtgc cgtccatctt ctcaaggata
     6721 ggtttgataa acttataaaa ttcctcttgg gaggcacctc cgtcgatgta gccggcgtat
     6781 ccattctttg actggtcaaa gaagatttcc ttgtacttct ccggaagctg ctgccggacg
     6841 agtgccttaa gcagggtcag gtcctggtgg tgctcatcat accgcttaat cattgaagct
     6901 gaaagagggg ccttggtgat ttcagtgttc acgcggagaa tgtcagacag caggatggcg
     6961 tcagaaagat tcttagcagc aaggaaaagg tcggcgtatt ggtcaccgat ctgggccagg
     7021 aggttgtcga gatcgtcatc ataggtgtcc ttggacagtt gcagcttggc atcctctgcc
     7081 aggtcgaaat tagacttgaa attgggagtc agcccaagtg aaagagcaat caggttcccg
     7141 aacagtccat ttttcttttc acccggcagc tgagcgataa gattctcaag tctgcggctc
     7201 tttgacagcc gggctgacag aatggccttg gcatcgactc cgcttgcatt gatggggttc
     7261 tcttcaaaga gctggttgta agtctgcacc agctggatga acagtttatc cacatcgcta
     7321 ttgtcaggat tgaggtcgcc ctcgatcagg aagtggcctc tgaacttgat catgtgggcc
     7381 agggccaggt agataagccg gaggtctgcc ttgtcggttg agtccaccag ctttttgcga
     7441 agatggtaga tggttgggta tttctcgtga taggccacct cgtcgacgat attaccgaag
     7501 atgggatgcc gttcatgctt cttatcctct tccacgagga atgattcttc cagcctatgg
     7561 aagaagctgt cgtcgacctt tgccatttcg ttggagaata tctcttgaag gtaacaaatc
     7621 cggttcttcc tcctggtgta ccgccgtcta gcagtccgct tgaggcgggt tgcttcagcg
     7681 gtctcccctg aatcaaagag gagagctccg atcagatttt tcttgatgga atgccggtcg
     7741 gtgttcccga gcaccttgaa cttcttagag ggcaccttgt actcgtcagt gatcacggcc
     7801 cacccgacgc tattagtccc gatggccagt ccgatagaat acttcttgtc cactttcctc
     7861 ttctttttgg ggctagcaag gatcctcgag ctcggcgcgc cttcgtgcca ttcgattttc
     7921 tgagcctcga agatgtcgtt caggcccccg gagcctcccc ctccgccacc acccgaacca
     7981 ccaccaccac caccacctga accaccccct ccgcctcccc cactgtacac attgtcaaga
     8041 tcaggagggt cccttgcggc agcaccgatc ataatctgcg cctcccgagt gcgagagatc
     8101 gctttagcca gcagctcgcg tgctgtatct atatcaacta taggcttagc gtaattggtg
     8161 cccagctcca ccccgcttgc cttgagcact gtcagtggtg cgtcccaagg atggtgaatc
     8221 cactccgtag gaagtcgggc cagctcgggg agccactgtc gaatatactc tccttcggga
     8281 tcatacttag ctccctgcaa ggccggattg tccaacctgt ccaactcatg gccgtcagga
     8341 attgacccgc taatatattg ccatcccaga atgtcacact ccaagtcggc gtcgagaagg
     8401 gtatcccaaa agtacttcat tccccatttc caaggcagga gcagaaactt gacagcaaat
     8461 gaggacacga ttacccggat gcggttgtgc atccagcctg tagcccagag ctctctcatg
     8521 ccagcatcca cgagtgggta ccctgtcctt ccctgtctcc atgccttgaa tttgtcgaca
     8581 tcggcgtccc aggggaagaa cctgaggtgg gacaacagac tctgctcgtg tgtgaatgga
     8641 aagttgaaac atatataccg ggagtattcc cgaagtccga tccctcgcag gaacagatcg
     8701 gcgctttctt cgccttcgct gtttttatcg cgagcccaga taatctgttt catccgagcg
     8761 cactgaaata catggcggac agaaatctcg ccaaaatgga gatatggaga gagcaagcta
     8821 gttgagttac ctaccacttt cttggagttc ttcgcgtagt caatcagttg tttttcgatg
     8881 aactcgttaa gcagcttgtc ggcattagac cagccggggc tccaggctct agtgagaagt
     8941 gcattgcttg gcttttctgc ttcattttcc agaccgagtt cctcgatgga gcaggcccag
     9001 atggcctctg cagcagcagt aatcggcatc agtctccagg gagggggcaa cataacggac
     9061 tctattgaca tatccaaaca tttcttccag taactgttga aggaggtgaa cggtttccct
     9121 ttctcgcaat agatctccca tggctcgtac agaaggtccc cgttgtagct ctgcacactg
     9181 atcccccgtt ccaccagctt ctccttcaca gtgtggtcgc gcaccagact aactggatcg
     9241 tacaggtggt taaacacgac tttcgttgcg ccggtcaccc tgatgcagtc gaggatggca
     9301 gagatggtat tgtgtgtctt gatcagggta agatcactgc ccagtgcttt cagtgactgg
     9361 ctaagatggg ccagactttg cttcatccac cacctagagg cccttccggg ataaaactgg
     9421 ccttcttctt caggacacca aatgaaaacg gggaagacgg aaccctcgtg ggccgcggcg
     9481 gcgagcgcgg gattatcttc tattctcaaa tctctccgaa accagacgat ggtctttttg
     9541 tccatcttca tggtggcgac cggtggtccg ctacccttgt acagctcgtc catgccgccg
     9601 gtggagtggc ggccctcggc gcgttcgtac tgttccacga tggtgtagtc ctcgttgtgg
     9661 gaggtgatgt ccaacttgat gttgacgttg taggcgccgg gcagctgcac gggcttcttg
     9721 gccttgtagg tggtcttgac ctcagcgtcg tagtggccgc cgtccttcag cttcagcctc
     9781 tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga ggaggcctcc
     9841 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
     9901 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
     9961 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
    10021 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta catgaactga
    10081 ggggacagga tgtcccaggc gaagggcagg gggccaccct tggtcacctt cagcttggcg
    10141 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
    10201 ttcacggagc cctccatgtg caccttgaag cgcatgaact ccttgatgat ggccatgtta
    10261 tcctcctcgc ccttgctcac cattccaccg ctagctcctc cacctccacg gtcctgctgt
    10321 ccatagccac cgctgccacc tccaccactc tggtcttgat tgccataacc gccaccactg
    10381 ccaccaccac tactcatgga ggattgatct tggccatagt tacctccacc tccacctcca
    10441 cctccaccac cactgctgct gttgtactgg ttctgctgtc catagccctg agggggatta
    10501 tagctttgct gctgtccata gctttgctgc tgtccaccat agctaggctg ctggctgtag
    10561 ctcccactct ggggctgccc atagctgctg ctctgagaac tgctaccgta acttcccgag
    10621 gtgctgctgg gagctggctg ctggccatag ccaggatagg aggactgctg cccgtaagac
    10681 gattgggagc tctggctact gccatagccg ccagtcgagc catatccctg gggagttgac
    10741 tgagttccat agcctgtgtt ctggctctgg ccataagaag aatagctgct ctggccatag
    10801 cctgaagtgt ccgtggactg gctataacca ctgtaactct gctgtccgta gggctgactg
    10861 ctctgctggg aatagccctg cccgggctgg gtggggtagg ccccatagct ttgggttgct
    10921 tgttgggtat aatcgtttga ggccatgcta gcggatctga cggttcacta aaccagctct
    10981 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
    11041 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
    11101 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
    11161 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
    11221 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
    11281 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
    11341 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
    11401 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
    11461 atttaccgta agttatgtaa cgcgataaac tggatctctg ctgtccctgt aataaacccg
    11521 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
    11581 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaggc
    11641 gataccgtcg agatccgttc actaatcgaa tggatctgtc tctgtctctc tctccacctt
    11701 cttcttctat tccttcgggc ctgtcgggtc ccctcggggt tgggaggtgg gtctgaaacg
    11761 ataatggtga atatccctgc ctaactctat tcactataga aagtacagca aaaactattc
    11821 ttaaacctac caagcctcct actatcatta tgaataattt tatataccac agccaatttg
    11881 ttatgttaaa ccaattccac aaacttgccc atttatctaa ttccaataat tcttgttcat
    11941 tcttttcttg ctggttttgc gattcttcaa ttaaggagtg tattaagctt gtgtaattgt
    12001 taatttctct gtcccactcc atccaggtcg tgtgattcca aatctgttcc agagatttat
    12061 tactccaact agcattccaa ggcacagcag tggtgcaaat gagttttcca gagcaacccc
    12121 aaatccccag gagctgttga tcctttaggt atctttccac agccaggatt cttgcctgga
    12181 gctgcttgat gccccagact gtgagttgca acagatgctg ttgcgcctca atagccctca
    12241 gcaaattgtt ctgctgctgc actataccag acaataattg tctggcctgt accgtcagcg
    12301 tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa gaacccaagg aacaaagctc
    12361 ctattcccac tgctcttttt tctctctgca ccactcttct ctttgccttg gtgggtgcta
    12421 ctcctaatgg ttcaattttt actactttat atttatataa ttcacttctc caattgtccc
    12481 tcatatctcc tcctccaggt ctgaagatca gcggccggcc gcttgctgtg cggtggtctt
    12541 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
    12601 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
    12661 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
    12721 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
    12781 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
    12841 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
    12901 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
    12961 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
    13021 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
    13081 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
    13141 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
    13201 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
    13261 ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta
    13321 gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg
    13381 aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc
    13441 atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt
    13501 atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc
    13561 tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag
    13621 atcaaggata tcttgtcttc tttgggagtg aattagccct tccaactact aagtttgtag
    13681 tacatattta acaaatacaa tttctttaaa atgaaaataa ttcagaggaa tcacaggttt
    13741 agagtaaatg aaaccacagg taattggcag tggtaatagg gtatggggtg ggaagtttgg
    13801 gatgattttg gttagcttga gttatccagt tgatccagac atgataagat acattgatga
    13861 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
    13921 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgatc tcccgatccg
    13981 tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
    14041 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
    14101 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
    14161 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
    14221 gaagatcag
//
