LOCUS       sequence-476303-       13058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GAL4 under control of the stmn1b promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13058)
  AUTHORS   Moyer AJ, Barcus A, Capps MES, Chrabasz JA, Lalonde RL, Mosimann C, 
            Thyme SB
  TITLE     Genetic context of transgene insertion can influence 
            neurodevelopment in zebrafish.
  JOURNAL   bioRxiv [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904.
  PUBMED    40093151
REFERENCE   2  (bases 1 to 13058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 Mar 5:2025.02.28.640904. doi: 
            10.1101/2025.02.28.640904."
COMMENT     Sequence Label: pTol2-stmn1b-KALTA4
FEATURES             Location/Qualifiers
     source          1..13058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1785..1803
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(3656..3667,3668..3830)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             8559..8999
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    9184..9318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(9620..10333,10334..10336,10337..10339))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(9623..10333,10334..10336,10337..10339))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9665..9686)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     10014..10033
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10273..10294
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(11638..11825,11826..11837)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11871..11887)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11871..11887)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    11910..11979
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(12029..12049)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12029..12047)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12068..12084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12068..12084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12081..12103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12092..12108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12116..12122,12123..12140,12141..12146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12161..12182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12299..12316)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12470..13058)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12550..12569)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     1801 agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct gaaaacttgg
     1861 atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt atttgtctgc
     1921 atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg actgtgttta
     1981 acagacatta ttacaataat atgtaatagt agtacacact attattatgt aatagtactt
     2041 gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca aaatgactta
     2101 cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta tggacatcgc
     2161 cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc gagtctgagt
     2221 caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga agtccacagc
     2281 ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt ggatgtgaga
     2341 gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa cttgggctac
     2401 agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat aaagatttta
     2461 atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt ttggatcact
     2521 agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt tggttggttg
     2581 gtagattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     2641 attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga ttcattacag
     2701 aaatgattaa atgcatacat aaaaaactgg gggggggggg ggatacaaca acacacttaa
     2761 gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat agctatattt tacatctcct
     2821 ttttttgctg tcttttaatt agccttgttt tgctgttatc ttgattgaaa ctgtaacttc
     2881 ttcacctgct tcttttcttt gtaggttttg ccagcccaga aacaggcatg gctgtgcaag
     2941 gccagagcac catgcacaat gcgctgcatg tcttcatgaa cggctcaatg tcctcagtcc
     3001 agggctcagc caacgacccc atcttcctcc ttcaccatgc tttcattgac aggtaacaaa
     3061 cacgtcatga cattagactg cacagttttt gacaaagttc atacaatctg ttgtttatag
     3121 ctgctacaat tagtgaagtt tgtgaatgta cttggatgag cagcgaaaga tcaattgaga
     3181 tcaattgtta gagtttggtt gccctgcaga gcaaagaaca aaaaataatc tggtggcttt
     3241 actgcgtgag gttattattg gtggaataga aacacaaaac ataattgcat ttatttgttt
     3301 aattttttat cttatcttaa ctttcatctt gcatatttgt ttcttacatc atttctagca
     3361 tctttgagcg ctggctaaga actcatcagc ctccccggtc catctaccca cgtaccaatg
     3421 caccaattgg ccacaatgac ggctactaca tggtgccatt ccttcctctt tataggaatg
     3481 gagactacct cctgtccaac aatgctcttg gatacgagta cgcctacctg ttggacccag
     3541 gtcattgcac aacaccagaa atgccctctg atctgcaaaa gacgtgaata atctgttcag
     3601 acacccatat gcactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     3661 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     3721 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     3781 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     3841 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
     3901 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
     3961 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
     4021 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
     4081 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
     4141 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccattc
     4201 gtacgcttaa tttcgtatag ggataacagg gtaatgatct aggttgccct cagtgatagc
     4261 agccaaaaaa tgtcccaaca gggggtttct cagctctctt tgtgtctttg tgtaagacac
     4321 cagagactcg agacagggcg aggcatggaa gcaccatctg ctcccccttt ccctgggcag
     4381 cttgctttaa aatgtccccc ctcaaacggg atgaatggga tccggggtgg ggcaggagga
     4441 ggctggcaga gcatgataca gataaaaaaa attgtgatct ttatgcaaaa taatcaagca
     4501 atacaatcaa caccatcaaa aggatagatg atatgataga tgaaatgata aattacattt
     4561 actcactatc tattgatcct caagtggtta aacctttata agtttcatac ttcaaaaaaa
     4621 taaaacactt tgaagaaagc tgaaaagctg taagcattga cttcctcagt aaacaaatat
     4681 catggaagtc agtggttaca ggtgttcaac attttccaaa atatcttatt ttgtgttcaa
     4741 cttcaaaaga aacttataaa ggtttgaaac aagtaaaatg atgacagaat tttcattttt
     4801 gactaaacta tccctttaaa taatgtattt tcttcacttt ggtgaagaat ttggagctaa
     4861 ggaatgatca caccacaata ttaatgcatt taaagagtca aaacaaacga ctccaacagc
     4921 atttagtgac acaggaataa acagcagcca caagactgta aaatccagtc agcttgaaat
     4981 catcagctct gccatttagc taaagttcat acaatgatac tgaggcacag agaagaacag
     5041 aagcaaaaca ctcactaatg ccaagtgaac tgttaattgc ttcgcaccga agacaatttg
     5101 taaagcaggt caccggcgtg tcgctgtttt gttctaagaa cttattgact gtatagtcat
     5161 ctctgacatg tcgcgagatg actaatacca ggggtgaggt caggactcac tttttcctaa
     5221 taaagcttca tgtgattata gtttgtaagc tggtttttga gaaaaaataa atgtgcttat
     5281 gacaagttgt catgtaggta aatcacttta cattatagcc ttacaaacaa agctctaaca
     5341 taatcagaac tgacccaact gtgcattatg tgagcgtcaa attcaagctc aagttgatat
     5401 ctttcactac acagcctact aataatgttg gtgaataaaa caaaacaaaa tgaacttgaa
     5461 gctacttaag tgtgatgtat gtgactaaac aaaaatgctt attgtgttga acaggtctcc
     5521 caacttgact cagtctccat ctgctactgg tcagtttaac tgaaagcccc acccaaaacc
     5581 cacaccattg gttaagccag tgtcactgtg ctggtcaaat cccaattcta aaattgtatg
     5641 aaaaatgttt tatagccaat gtcttatata caaaagctat agaggtgagt tttcactttt
     5701 aaccacaata aacacaatat ttgtgcactt taaagttaaa gtaacattta aacatttctt
     5761 gacatgacct tttatcatat cattttttag ttaatctgcc ctaaaaacat catgtaaaca
     5821 aaaactgact taaaacttat gctaatacac tttagcttta aatgagcaat gtctttttaa
     5881 acataggcta cccaacagct acccgaaaat attagttagc ataaacagta cattgtcact
     5941 ggataaaaca atgacaaacg gcaacgctat taaacatttc actacataaa tgatgacata
     6001 ttaactgtta aaacagagca gtagttttta cacatatttt catacaaagg gcatttttgt
     6061 cagcaatcgt ataatttgat gcttctcgtc atatataggt taactgcatt tctatttaga
     6121 ataatatagt agaagcatac ttcctaagtg tgaagagggt catactaatg gggtatatcc
     6181 acactgactt ttaatttcag tcatccagcc acatggcttc cggttaattg tcatcccata
     6241 caaaaaacgt tgatcttctc tgcctggctg agatacgata taaagtgagt atcaaagaaa
     6301 aagcactaca ttaaagtata tttttccacg tcaagtcttt ataatatgga aattaatttt
     6361 gtgccagtat tttcaatgga atttctgcgc tagcctgttg ctaatgacgt cgctagcatt
     6421 tacaccattg ttttacagtc tttcgtctct ttttagcttt aacaattaaa tgaatgatta
     6481 ggtctattta agtcatttga actacattac aacattgatg ctgtaaaagg aattaaattg
     6541 aaaatagtca cataaagctt taatcggaag ttcatcattg gctgaaaaac actagctgtg
     6601 catttagtac atttaaaagc agtttatgtt aggacaatga acttatttta ccgttcaaac
     6661 gataccgtac tacatttccc agaattctgc tctcgacaat cattggtctc atccaaaagt
     6721 gacgtaatac attttggtcc tcttttgcgc gaagcgaacc gttgatgctg aggcagagag
     6781 gaatctgcgc gcgggcaact ccttccctga tttccacttc ttctgaaggt gggtgttttt
     6841 ttttgttgtt gttgtcaaat atcactattt ttgcagcgca tatcccatcc cttccttacg
     6901 tgtcaaacag cgaatgcatt taaagagtaa acttttaaat tcttacaatt ataatgttct
     6961 gccacaccgt tatttacctc cttttttggg gaggggatgt acaacgttag gtaacgttaa
     7021 tggacaaggg cttcgagaat attcagttcg gttggagagt ttcagtaact aacgttaagt
     7081 ctgtgaattt aaaaatagca cgcgcagtcg agtaacagct cgtagcggga ccattaatgt
     7141 gtttatcgtc gttatatctg gagggaattg gtcgagatta gccagctggc tgtcgtttca
     7201 tcgagttttg caggtttatt tttgactgtt tggtgttgca aaaacgtgct ttattgtcat
     7261 ccacagactt atcgtctgtt tcacttaaat cttaacaagc atacaacaca atgtgacagg
     7321 tgatctaatg gctctcgacg tcttttgtct tcgttatttg tgcattcgcc aactatgccg
     7381 tcatattata ttatttatat tcacatgctt caataaaatc gatcagtaat ggcatagcac
     7441 gctgcatttg gcataccaac actgattatt ctgtaaaaaa ggcagtgcag ttgtgtctgg
     7501 tttgaaacgg tagagatttg ggtgtgatct ggtgtgctgg aaacgggtga ttcagccatg
     7561 gctttattag taaatgttgc atttcaaatg tgcatattct aaaacattat taagctcttg
     7621 gtcatcatgc aataaagtgt tgtatgtaat attatgtatt tttgcatgct gttataatgg
     7681 gtgtatctcg gccatctatt gtctgcagat gagatctttc atccctccgg ataatcgcta
     7741 gtgttggatg tttattttca gccatcgagc tatgaattgt tcacaggtca aattttgaga
     7801 tgacaagttc gtctaataat aaatatttga cgattctcca gggggcatta gctcgaggat
     7861 caatgctctg ttgtctttgt tcgcttctta cacaatatct cctttgtttt aatctctcaa
     7921 tggccaattg aatcacgtta cattataaaa aaagaggcag cgttgtttgc tctacatttc
     7981 accagagcgg tcagcctgtg ttgtatattt taattgaact attatcgttt aattagacga
     8041 tgatgacagc acgtctgaat ggaaggattc ggtttttctg ctggcagtga ctcgtctttt
     8101 tttcttggct ttcagcaaaa ggaggatata ttggcatgaa ttaggctgta tttttgttgc
     8161 atttttaacg agtattgcac agtcttgcct atttccttac cgtaaaacaa ttcacttagt
     8221 aaattatgca tgtctattgt ggcgacgccc ctcccttaag cacgtgtaca ttattacatt
     8281 atataaacat cgtttcctat cttgtaaaaa tgttatagta ggcagcaggg actgactcca
     8341 gtcatcattt ttcataaatg tctaccttaa acatttttca ttcattgatt gaacactgct
     8401 gtatagatgt ctgtttacaa tacattgtgg ttaaatatgg ttgagtgaaa agattgccta
     8461 gacattgttg ttaacagcaa aacgtttcca tttttctaac atcattatta tttacaatca
     8521 tttcgcaggt gtctgaaacc gtcgaagtag aaagtgcaat gaaactgctc tcatccatcg
     8581 agcaagcctg cgacatttgt cggcttaaga agctgaaatg ctccaaggaa aagccgaaat
     8641 gtgccaaatg cctgaagaac aattgggaat gtcgttactc tcccaaaacc aagcgaagtc
     8701 cactcacaag ggctcatctg accgaagtgg agagcaggct agagagactg gaacaactct
     8761 ttttgctcat cttccctaga gaggaccttg acatgatcct caagatggat tctctccagg
     8821 atattaaagc ccttttgact ggcttattcg tccaggacaa tgtgaacaaa gacgctgtga
     8881 cagaccgatt ggcaagtgtc gagaccgata tgcctctgac actgagacag cacagaatca
     8941 gcgctacttc ctcaagcgaa gagtcttcta acaagggaca gagacagctg actgtttcga
     9001 gcaggtcgac cccgtccccg gccgacgccc tggacgacgg cgacctggac atgctgcctg
     9061 ctgatgctct cgatgatttc gatctggata tgctcccggc cgacgccctg gacgactacg
     9121 acctggacat cctcccgggt aactaacaaa ctcgagaaag ctagcaaacc atggtacgat
     9181 gatgatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     9241 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     9301 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     9361 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     9421 gatcctctag atcagatctc ttgtttattg cagcttataa tggttacaaa taaagcaata
     9481 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     9541 aactcatcaa tgtatcttat catgtctgga tctacgtaat acgactcact atagttctag
     9601 aggctcgaga ggggccgctt tacttgtaca gctcgtccat gccgagagtg atcccggcgg
     9661 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     9721 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     9781 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     9841 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     9901 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     9961 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
    10021 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
    10081 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
    10141 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
    10201 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
    10261 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
    10321 cctcgccctt gctcaccatg gtcactgtct gctttgctgt tggtctgggc tcctgggtca
    10381 ctggcttact aatggagtct ttatgtatga ggactcttat caattgttct tctataaagg
    10441 tctgcagtgt ttctgttcgt cccctacatg gacacccaga gcctcctaaa tacaggagcc
    10501 ctgataactg cacaagtgct cagattccag cagggtggaa aatgagataa agtgtgcaga
    10561 tggggagggg gacgtgaatg agagatttga gggatgaaaa ggatggatga acgcattgaa
    10621 aatagcccct ttcacaaaat aataccagta aattgtcata taattaatga ccagaccttt
    10681 acctgtaaat gatgtgatgt tcatatacag aagaatgcca acaatttaca gatgatttta
    10741 caaattctta attttccttt tctaaatgga tttgttcaca cattatctct tcaaagcaat
    10801 tgaattttct ggaaaagatt ggctgtgtgt gtaaaaggtg ctactgagga atttaatgtg
    10861 acatggaatg aagcaaacag cagcctaaga caggatggca ggaaaaatgt cacatgttta
    10921 aaataagagt gaaaccaacg acctggactt atagagactg tgtgtcctta ggcaaagctc
    10981 ttgtacaaac agtgcttgtg actgtgaaat tcggggtttg cctggattgt gttaaatgtg
    11041 tgcttggatg tatcataaaa aagaatcggt tctctgtatt tgacattttc tgtaatttat
    11101 ctaaagttac aactggctat gcctgatttt tatcactatt gaatcaatca ttttaaagaa
    11161 tgttaagtct gctgatttgg agagctcatt tacatttatt ttgaatgtct cttatttaac
    11221 acaactgatt taagctttag atctaatctg atcattcagg cgcgccaaca cgtgaatacc
    11281 acgcgaggcc ttagggataa cagggtaata cgcgtgatct gcgaagatac ggccacgggt
    11341 gctcttgatc ctgtggctga ttttggactg tgctgctcgc agctgctgat gaatcacata
    11401 cttcctccat tttcttccac tgattgactg ttataatttc cctaatttcc aggtcaaggt
    11461 gctgtgcatt gtggtaatag atgtgacatg acgtcacttc caaaggacca atgaacatgt
    11521 ctgaccaatt tcatataatg tgaaaacgat tttcataggc agaataaata acatttaaat
    11581 taaactgggc atcagcgcaa ttcaattggt ttggtaatag caagggaaaa tagaatgaag
    11641 tgatctccaa aaaataagta ctttttgact gtaaataaaa ttgtaaggag taaaaagtac
    11701 ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt attgattttt aattgtactc
    11761 aagtaaagta aaaatcccca aaaataatac ttaagtacag taatcaagta aaattactca
    11821 agtactttac acctctggtt cttgaccccc taccttcagc aagcccagca gatccactag
    11881 ttctagagct cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg
    11941 cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatctg atgaacgggc
    12001 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
    12061 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    12121 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    12181 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    12241 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    12301 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12361 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12421 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12481 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12541 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12601 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12661 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12721 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12781 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12841 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12901 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12961 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    13021 gcaagcagca gattacgcgc agaaaaaaag gatctcaa
//
