LOCUS       sequence-476309-       19727 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas12i1-TAD system backbone (Cas12i1-TV) for gene regulation in 
            rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19727)
  AUTHORS   He Y, Liao S, Ren Q, Tang X, Zheng X, Qi Y, Zhang Y
  TITLE     CRISPR-Cas12i confers efficient genome editing and gene regulation 
            in plants.
  JOURNAL   Plant Physiol. 2025 Apr 30;198(1):kiaf125. doi: 
            10.1093/plphys/kiaf125.
  PUBMED    40305671
REFERENCE   2  (bases 1 to 19727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-04-30"; volume: "198(1):kiaf125. doi"; pages:
            " 10.1093/plphys/kiaf125"
COMMENT     Sequence Label: pGEL1020
FEATURES             Location/Qualifiers
     source          1..19727
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(38..105)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(547..583)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     terminator      complement(2147..2395)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(2420..2569)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(2582..2731)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3293..3304
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(3764..3811)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(7112..7132)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      7135..7144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9182..9198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9182..9198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9195..9217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9206..9222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9230..9236,9237..9254,9255..9260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9275..9296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9487..10163
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10100..10120
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10230..11255
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             10230..11255
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    11296..11470
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11548..11572
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11997..12791
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14216..14804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14705..14724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14958..14975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14990..15130
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15056..15075)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15216..15238
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15474..15668
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15734..16807)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(17236..17865)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    19166..19190
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     19378..19400
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     19392..19409
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19393..19409
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccaatcca gaagatggac aagtctagga ctcgagtgtc ccattcgcca tgccgaagca
       61 tgttgcccag ccggcgccag cgaggaggct gggaccatgc cggcctgaga ccaagccaga
      121 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
      181 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca
      241 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac
      301 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga
      361 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag
      421 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      481 ttgtggatgt acaagagtga tattattgac acgcccggtc tccgtgccaa cgatgggcac
      541 aaaaatgacg agcttactcg tttcgtcctc acggactcat cagatttttc ctgcaggctg
      601 cacacacaat taccgaagca tggtcagcaa ctatgaacat gaaattataa caataccaag
      661 caagtacagc agaaatcagt caatcaaatt attccaatat gttagataca cgatctaaac
      721 cacaataaca tcagtgtacc tatcaacacc aacagtgacc taacaaaata gagagtcgca
      781 ccgtgctcga gttcacaaaa ataatctgca ggtctatgaa ctactagaat tccatcggca
      841 aactgattca ataactacta gcactatccc gagagcacat aaggcaggtt aattaacata
      901 ggctagaatt tacaggtgca acagttgata agataggcat aagcaagtaa aatatcctga
      961 aagctatgct atgtaatcac taatcaacaa gacgcagccc ttagattaaa catgaacaga
     1021 gtagaatcta tgcaactaga gggactgaat caaaaacaac ctaagcaaaa tatacctgaa
     1081 gctgctactt ggatcaacga gaaaaacaga gactaaatcc tactgattga acagatcgga
     1141 acaaatcaca accctagcac agttctactt ctagatccga accaataaat acttggacca
     1201 cgaacagcat cgctcataat cacagatcca gcataccacg cgaaccccat ctaaccctgt
     1261 tcaaatctac cacagatcgc aaaacggaga ctaacaacaa catctcacaa tcacgaacag
     1321 gccgggaaat caaatcaaac gcgaggaacg gagaggatca gaaaatcgca taccttgaga
     1381 aggcgattcg acgcttcgct tcgaggggag attttttttt ctctgcgatt ttggggattc
     1441 gattgatgga ggaagaggcg aggagggggt ggggtattta taggtgggga gggggggact
     1501 cgtgagtggg cccaggggtg cggtgggtgg ggcccagtga gaatatagac aaataaagag
     1561 agggagaggc ggtttgggga gtccgtcccc accacgcaaa cgcgagaagg aaagaaagga
     1621 acggaaaggg ggcgggtcca gacggcgccg cggagatatc gaggaggtgg aaagctgatg
     1681 ccgtttgctc acggcggggt tggtggccgt taacacgcag cgaagcggcg ttactgccgt
     1741 gtacgttttg tgacctttcg aaagagagcc ccagcccaag aaaggctggc caggtttttt
     1801 tgtactgtgt acgattacga cggaagaaaa catactcgtt gttttgtatt ggtagatact
     1861 gggccgtagt aaaattagaa tcagttgtta attggttatt atatatacca tcttaagcta
     1921 ccaaaattaa taaagttgtg atggctatgg tatattttgg tagtgaatta tattgatttg
     1981 gtatatatta gaatcaatcc aaccacaaca caaccaaaaa tacctgaagc tcaatattga
     2041 tggtgctttt cgactatcta gcaaatgtgg tgaatggggc tttataattg aagcagccca
     2101 ggatgcactt tgtgcagaga ctgatgtggg cgcgccgtcc gagctcctta tctttaatca
     2161 tattccatag tccataccat agcacataca gtagttatat gctgcagaag agatccaaca
     2221 aaacattcac aatggattat agaaacattt gtttattcat tataatgtga tcttacattc
     2281 atttaatatt agaaaaagcc acaaattcat aacacaacaa gccaagaaaa aaacacaaac
     2341 ttaagcacac aagcttttta tttgacacac caaatatttc atcttcatct tcatatactc
     2401 gagtacgcgt tcaccgggcg agcatgtcaa gatcaaaatc atcaagagca tcactgccga
     2461 gcatatcgag atcaaagtcg tccagggcgt cgctacctaa catgtcgagg tcgaagtcgt
     2521 ctaatgcatc agagcccaac atatccaggt cgaagtcgtc cagcgcgtca gaccctcttg
     2581 ccagcatgtc caaatcgaag tcgtcgaggg cgtcggaccc caacatatct aagtcaaaat
     2641 catcgagtgc atcgctcccg agcatatcga gatcaaaatc atccagtgca tctgatccga
     2701 gcatatccaa gtcgaaatcg tcaagggcat ctgagccacg tgcttgtggg agaagctcca
     2761 tcagccatgc aagctcttcc tcgttaaaag ctgggaagtc gtcagcagtc cctgcaaaag
     2821 gatcagcgtc ctgttcccag accaccgtgc tggaggcaac aagatcagca tccatcggcg
     2881 tgccggggtc taaaagaccg ccggagccac cctgcggcag cagctccatc agccacgcca
     2941 gctcctcttc attaaacgcc gggaaatcat cggctgttcc agcaaatggg tccgcgtctt
     3001 gttcccatac aactgtggaa ctggcgacga ggtcggcatc cataggagtt cctggatcga
     3061 gtagcccacc agaaccaccc ctggcctgcg gcagcagctc catcaaccaa gccagttcct
     3121 cctcgttgaa ggcggggaag tcgtccgctg tgccggcaaa ggggtcggcg tcctgctccc
     3181 acacgacggt ggaggaggcc acgaggtcag catccattgg tgtcccaggg tccaaaagac
     3241 cgccagaccc tccttgagga agcaattcca taagccaggc cagttcttcc tcattgaagg
     3301 ccgggaagtc gtcggcggtt ccagcgaacg ggtccgcatc ttgctcccag acgacagtag
     3361 atgacgcaac taaatccgcg tccatgggtg tacccggatc gagcaatcca ccactgcccc
     3421 cgcgcgcctg aggcaacaat tccatgagcc aggccagctc ctcttcattg aaagcaggga
     3481 aatcatctgc ggtgcccgcg aatgggtctg catcctgctc ccaaactaca gtcgacgagg
     3541 ccaccagatc agcgtccatc ggggtgcccg ggtcgaggag gccccctgat ccgccctgtg
     3601 ggaggagttc cattagccaa gcaagctcct cctcgttgaa cgcaggaaaa tcatccgccg
     3661 tgcctgcgaa gggatcagca tcttgctccc acaccacggt tgagcttgca actagatcgg
     3721 cgtccatggg cgtgcccgga tcaagtagcc ctcccgatcc gcccttcttt ttcttagcct
     3781 gtccggcctt tttggtggca gcaggacgct tttttttctt gaaatccttt tggatatcca
     3841 caagcccgac attgtacgcg gcgacgaggt cagcattgca cacccaaaat tgttttccgt
     3901 tccagttaac ggacgtggcg tcagcatcaa gcttgtacgt agccaaataa aacatccccc
     3961 cacgtgacgg gaataggagc tgatcctctg atcgttggtg gagaatattt gccatgatct
     4021 gtttgaattt ctcgaggcta acaccgagga cgtcgttctt tttcaggcca taggtagcga
     4081 gaaacgccat ggcgccttca ttgtaatatg cattggtcgg cctcttactc ggcttatcgc
     4141 taagaaagct caggatctct ttccggttct tttcggtgag ctcggagggt gtacatctcg
     4201 aataccgtgc ccggaatgtg ttttcagggt tcttgtgcac aaagggatcc tgatggctcg
     4261 tgaaattagg gttgacctcc acaaactcca gaccctggtg catcatcacc atttccttca
     4321 ccttattggc tacgccgcgc gctagccaat ccatcaggaa agaattagta cttgattttt
     4381 tccccttctt ggtggtatca ctaatgtttt cgcctttgat gagaactggg ccgtacttgt
     4441 cacgcaattc aagtgctttc gcgacaatct tgttagctat cacctccttt ttactcttga
     4501 ctttattcaa cctcttttct tctagcgcaa gtctcaaagc aaacatctcc ggatctgcct
     4561 tctgtttatc ctcatctgtg ataggaggcg ccttaacgtc ggattttgaa ttctttggct
     4621 tctttaacag gtgcccgaag tatgtcccga tgaggctctt agccaccttg aacataacaa
     4681 agcttaggtt tgacaaggaa gagagcttga ggacggaaag tttcccatcg cggagcagct
     4741 cgaatatctc ctccctgtac tccttagcct gcgagctgtg gttacgtatg gatgattgca
     4801 gtagcttaca atacttcatg ttaaagtaat acaatgaagt ctcatcatcg gcgatggcct
     4861 caaaatcctt catgaagtca atctgaatgt tgttaccgcg gttatctttc atcgtaccat
     4921 ttctatattt ctcgagaaag gacctgcgac tttcgacatg tggtttgcag tagaggagct
     4981 tgacgccatt gtatgagagc tgatcgtaac ttttgtcgcg aacttgtgac tctacggtca
     5041 cgaaacctga ttcaatgggc tttaccggca ggtcattgta gtcaattacc ccatcaccgt
     5101 tcgcgtgtgc ttcaattgca gcatatgtat tctttctaac tatatttgcg tcataaccga
     5161 gcacaacttt ataattcttt tccttctttt ttttcacttt tgtggcgagt gtgacttcaa
     5221 agttgttgcc cctcttgcat atattaatgt tgaaatcctt tccccacgta taacgcggca
     5281 gcagattttg ttgccgcgcg gcttccaggc gcatttgcct ctttttaacc ttcctcaagc
     5341 cgactggcgc cgaacgtatc tgttcaattt gctctgcaga aagcgcaggc ttgccctcgt
     5401 agccgtttgt cttagtcctg aaccgggccg ccagtgcgtc cggcggattt tcggaagtcg
     5461 ccgggtagta gacctcttcc aagaatcgtg tggaagacaa cgcgtagtgg tgtttttccc
     5521 atctcatggt tttcgtattc agtaccttta tttcgatcca aatgtagtta tcaacctgat
     5581 ttctgttaca tttaagatta tgtttaagct tgtccttggg cgagataatt tttcccagca
     5641 acttagagtt gttcccgaaa ttaaagctag ggtacttctg aataaccggg ttaatcttct
     5701 gcttctcaac cttatgcttc ttagacagaa aggtgatgcc atcgatgatc ttgtcgacgg
     5761 cgatgtcgtc cttgcgagag tagaggtacc tcaacagttg cactagcgga gttttcgcct
     5821 ccttgcccac ctcctcgcaa taagtgttga tggtctggca cacgtccccc tggtacactt
     5881 tgattcgctt ttccttctta tcttcggata gggatgcaat atcctcttca tacttcgaat
     5941 ggatcgcgtt taaatcctcc cataaagtct tgatattact ctcgccgatg tgatgagggt
     6001 gcagcaccca cttaacattg gtgtcgctct cgaagtatgg ggagtcgaaa aatccttcga
     6061 cgatcttgcc gaagggctca ttctcatatt tgatatcatt cttagcgtcg atttgcttaa
     6121 gtacgaactt acaatttttg gtggtcatgc ccttaattgg gctcaaggcg ttctccagca
     6181 tggcactata cgagccgaga tagtatggtc ccaccttgga tgcaagaaac gcttttatct
     6241 tctccatgca cttccactca cactcatatt cgaaccgacc aagcttctct ttgaggttag
     6301 ctatgaggct tgggacgttt acctcgtcgt catggttggt ccctagctcg taatcttgca
     6361 ctatcttgag aattgatggc gtggcgccct tgcgtgagca tcggatgcgg agcttccggt
     6421 agctgtcgca gtcaccagcc ttcaaaatga tcgaggctaa ttgctccacg gaattaatct
     6481 gtgttgattc ctccagaagt tggattacct tgctgtagaa ctggtgctta gtttgcctat
     6541 tattctttgc tcctgttccg aacatgaccg acgtaatgta gtgggccgtc gagagagaca
     6601 atccaatgca tttttccttg tacatcaact tcagatcatg atgcatgtca gaccggctga
     6661 gctgcaactc cgccagaagc ctctcatatt caaccctcat atctttccac tgatgtttct
     6721 ctgtatcaaa gttactggcg aaatactcct gggcttggat gtcaggttca gatccactgt
     6781 actgctggaa caagttagcc aagttcgagg gagaaacgcc ctgcggttta gaatttattg
     6841 gcatgacttt gaaccaattc acggcaagaa ggaggtcatc agaaatcttg tccttgtttg
     6901 cagtcccgga gattatgtcg cggtcgatgc ctccgccaaa ctggttccac agttcgaaga
     6961 agataggcgt accatccata agatagtcca tgaagttacc gagcagcttc attctatcat
     7021 gtgaccgcgg tatcatctta gttgtgtaag cttttctagt ctcgctcgca tttttctcct
     7081 tattagaagc cgcaggcacc ccgtgaatac caaccttccg cttcttctta ggagccatgg
     7141 cggcctgcag gaagtaacac caaacaacag ggtgagcatc gacaaaagaa acagtaccaa
     7201 gcaaataaat agcgtatgaa ggcagggcta aaaaaatcca catatagctg ctgcatatgc
     7261 catcatccaa gtatatcaag atcaaaataa ttataaaaca tacttgttta ttataataga
     7321 taggtactca aggttagagc atatgaatag atgctgcata tgccatcatg tatatgcatc
     7381 agtaaaaccc acatcaacat gtatacctat cctagatcga tatttccatc catcttaaac
     7441 tcgtaactat gaagatgtat gacacacaca tacagttcca aaattaataa atacaccagg
     7501 tagtttgaaa cagtattcta ctccgatcta gaacgaatga acgaccgccc aaccacacca
     7561 catcatcaca accaagcgaa caaaaagcat ctctgtatat gcatcagtaa aacccgcatc
     7621 aacatgtata cctatcctag atcgatattt ccatccatca tcttcaattc gtaactatga
     7681 atatgtatgg cacacacata cagatccaaa attaataaat ccaccaggta gtttgaaaca
     7741 gaattaattc tactccgatc tagaacgacc gcccaaccag accacatcat cacaaccaag
     7801 acaaaaaaaa gcatgaaaag atgacccgac aaacaagtgc acggcatata ttgaaataaa
     7861 ggaaaagggc aaaccaaacc ctatgcaacg aaacaaaaaa aatcatgaaa tcgatcccgt
     7921 ctgcggaacg gctagagcca tcccaggatt ccccaaagag aaacactggc aagttagcaa
     7981 tcagaacgtg tctgacgtac aggtcgcatc cgtgtacgaa cgctagcagc acggatctaa
     8041 cacaaacacg gatctaacac aaacatgaac agaagtagaa ctaccgggcc ctaaccatgg
     8101 accggaacgc cgatctagag aaggtagaga gggggggggg gaggacgagc ggcgtacctt
     8161 gaagcggagg tgccgacggg tggatttggg ggagatctgg ttgtgtgtgt gtgcgctccg
     8221 aacaacacga ggttggggaa agagggtgtg gagggggtgt ctatttatta cggcgggcga
     8281 ggaagggaaa gcgaaggagc ggtgggaaag gaatcccccg tagctgccgg tgccgtgaga
     8341 ggaggaggag gccgcctgcc gtgccggctc acgtctgccg ctccgccacg caatttctgg
     8401 atgccgacag cggagcaagt ccaacggtgg agcggaactc tcgagagggg tccagaggca
     8461 gcgacagaga tgccgtgccg tctgcttcgc ttggcccgac gcgacgctgc tggttcgctg
     8521 gttggtgtcc gttagactcg tcgacggcgt ttaacaggct ggcattatct actcgaaaca
     8581 agaaaaatgt ttccttagtt tttttaattt cttaaagggt atttgtttaa tttttagtca
     8641 ctttatttta ttctatttta tatctaaatt attaaataaa aaaactaaaa tagagtttta
     8701 gttttcttaa tttagaggct aaaatagaat aaaatagatg tactaaaaaa attagtctat
     8761 aaaaaccatt aaccctaaac cctaaatgga tgtactaata aaatggatga agtattatat
     8821 aggtgaagct atttgcaaaa aaaaaggaga acacatgcac actaaaaaga taaaactgta
     8881 gagtcctgtt gtcaaaatac tcaattgtcc tttagaccat gtctaactgt tcatttatat
     8941 gattctctaa aacactgata ttattgtagt actatagatt atattattcg tagagtaaag
     9001 tttaaatata tgtataaaga tagataaact gcacttcaaa caagtgtgac aaaaaaaata
     9061 tgtggtaatt ttttataact tagacatgca atgctcatta tctctagaga ggggcacgac
     9121 cgggtcacgc tgcactgcag gcgcgccgat ccccgggtac cgagctcgaa ttcgtaatca
     9181 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9241 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9301 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9361 tcggccaacg cgcggggaga ggcggtttgc gtattggcta gagcagcttg ccaacatggt
     9421 ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag aagaccaaag
     9481 ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat tccattgccc
     9541 agctatctgt cacttcatca aaaggacagt agaaaaggaa ggtggcacct acaaatgcca
     9601 tcattgcgat aaaggaaagg ctatcgttca agatgcctct gccgacagtg gtcccaaaga
     9661 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa
     9721 gcaagtggat tgatgtgaac atggtggagc acgacactct cgtctactcc aagaatatca
     9781 aagatacagt ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg
     9841 gaaacctcct cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa
     9901 aggaaggtgg cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg
     9961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
    10021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
    10081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
    10141 ttcatttgga gaggacacgc tgaaatcacc agtctctctc tacaaatcta tctctctcga
    10201 gctttcgcag atccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
    10261 tcgagaagtt tctgatcgaa aagttcgaca gcgtgtccga cctgatgcag ctctcggagg
    10321 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
    10381 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
    10441 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
    10501 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
    10561 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
    10621 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
    10681 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
    10741 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
    10801 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
    10861 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
    10921 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
    10981 ggaggcatcc ggagcttgca ggatcgccac gactccgggc gtatatgctc cgcattggtc
    11041 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
    11101 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
    11161 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
    11221 gacgccccag cactcgtccg agggcaaaga aatagagtag atgccgaccg ggatctgtcg
    11281 atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag
    11341 ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
    11401 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
    11461 aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt
    11521 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
    11581 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
    11641 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
    11701 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
    11761 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc
    11821 aaaatcggct ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct
    11881 gttttctggt atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta
    11941 taattagctt cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg
    12001 ctaaaatgag aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag
    12061 atacggaagg aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat
    12121 atttaaaaat gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg
    12181 acatgatgct atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc
    12241 atgatggctg gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt
    12301 atgaagatga acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct
    12361 ttcactccat cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg
    12421 aattggatta cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag
    12481 acactccatt taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag
    12541 aggaacttgt cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca
    12601 aagtaagtgg ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg
    12661 ccttctgcgt ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt
    12721 ttgacttact ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg
    12781 aattgtttta gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact
    12841 gagcgctgat gaatccccta atgattttta tcaaaatcat taagttaagg tagatacaca
    12901 tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga
    12961 actcgatatt ttacacgact ctctttacca attctgcccc gaattacact taaaacgact
    13021 caacagctta acgttggctt gccacgcctt acttgactgt aaaactctca ctcttaccga
    13081 acttggccgt aacctgccaa ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg
    13141 attgttaggt aatcgtcacc tccacaaaga gcgactcgct gtataccgtt ggcatgctag
    13201 ctttatctgt tcgggcaata cgatgcccat tgtacttgtt gactggtctg atatccgtga
    13261 gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta cacggtcgtt ctgttactct
    13321 ttatgagaaa gcgttcccgc tttcagagca atgttcaaag aaagctcatg accaatttct
    13381 agccgacctt gcgagcattc taccgagtaa caccacaccg ctcattgtca gtgatgctgg
    13441 ctttaaagtg ccatggtata aatccgttga gaagctgggt tggtactggt taagtcgagt
    13501 aagaggaaaa gtacaatatg cagacctagg agcggaaaac tggaaaccta tcagcaactt
    13561 acatgatatg tcatctagtc actcaaagac tttaggctat aagaggctga ctaaaagcaa
    13621 tccaatctca tgccaaattc tattgtataa atctcgctct aaaggccgaa aaaatcagcg
    13681 ctcgacacgg actcattgtc accacccgtc acctaaaatc tactcagcgt cggcaaagga
    13741 gccatggatt ctagcaacta acttacctgt tgaaattcga acacccaaac aacttgttaa
    13801 tatctattcg aagcgaatgc agattgaaga aaccttccga gacttgaaaa gtcctgccta
    13861 cggactaggc ctacgccata gccgaacgag cagctcagag cgttttgata tcatgctgct
    13921 aatcgccctg atgcttcaac taacatgttg gcttgcgggc gttcatgctc agaaacaagg
    13981 ttgggacaag cacttccagg ctaacacagt cagaaatcga aacgtactct caacagttcg
    14041 cttaggcatg gaagttttgc ggcattctgg ctacacaata acaagggaag acttactcgt
    14101 ggctgcaacc ctactagctc aaaatttatt cacacatggt tacgctttgg ggaaattatg
    14161 aggggatctc tcagcactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    14221 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    14281 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    14341 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
    14401 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    14461 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    14521 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    14581 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    14641 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    14701 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    14761 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    14821 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    14881 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    14941 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagggc ctgatgcggt
    15001 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
    15061 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
    15121 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
    15181 cccggcatcc gcttacagac aagctgtgac cgtttccggg agctgcatgt gtcagaggtt
    15241 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
    15301 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt
    15361 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
    15421 accgaagggt aggcgctttt tgcagctctt gggctgtgcg ctggccagac agttatgcac
    15481 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
    15541 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
    15601 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
    15661 tatccacagg aaagaggcct tttcgacctt tttcccctgc tagggcaatt tgccctagca
    15721 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
    15781 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
    15841 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
    15901 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
    15961 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
    16021 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
    16081 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
    16141 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
    16201 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
    16261 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
    16321 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
    16381 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
    16441 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
    16501 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
    16561 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
    16621 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
    16681 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
    16741 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
    16801 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac
    16861 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
    16921 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    16981 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    17041 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    17101 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    17161 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    17221 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    17281 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    17341 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    17401 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    17461 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    17521 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    17581 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    17641 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    17701 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    17761 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    17821 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    17881 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
    17941 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
    18001 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
    18061 gagccgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
    18121 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
    18181 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
    18241 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
    18301 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
    18361 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    18421 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    18481 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    18541 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    18601 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    18661 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    18721 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    18781 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    18841 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    18901 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    18961 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    19021 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
    19081 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat
    19141 tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata
    19201 gtttaaactg aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc
    19261 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    19321 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    19381 agtcacgacg ttgtaaaacg acggccagtg ccacactgag ctcgatatcg gcgcgccccg
    19441 ggagctcgca aaacacacct agactagatt tgttttgcta acccaattga tattaattat
    19501 atatgattaa tatttatatg tatatggaat tggttaataa aatgcatctg gttcatcaaa
    19561 gaattataaa gacacgtgac attcatttag gataagaaat atggatgatc tctttctctt
    19621 attcagataa ttagtaatta cacataacac acaactttga tgcccacatt atagtgatta
    19681 gcatgtcact atgtgtgcat ccttttattt catacattaa ttaactt
//
