LOCUS       sequence-476312-       18967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas12i2Max variant backbone for gene editing in rice.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18967)
  AUTHORS   He Y, Liao S, Ren Q, Tang X, Zheng X, Qi Y, Zhang Y
  TITLE     CRISPR-Cas12i confers efficient genome editing and gene regulation 
            in plants.
  JOURNAL   Plant Physiol. 2025 Apr 30;198(1):kiaf125. doi: 
            10.1093/plphys/kiaf125.
  PUBMED    40305671
REFERENCE   2  (bases 1 to 18967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-04-30"; volume: "198(1):kiaf125. doi"; pages:
            " 10.1093/plphys/kiaf125"
COMMENT     Sequence Label: pGEL1019
FEATURES             Location/Qualifiers
     source          1..18967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     ncRNA           complement(38..105)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     ncRNA           complement(524..560)
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     terminator      complement(2124..2372)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(2391..2438)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(5622..5642)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5645..5654
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7693..7709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7693..7709)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7706..7728)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7717..7733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7741..7747,7748..7765,7766..7771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7786..7807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7998..8674
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     8611..8631
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8741..9448
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     9154..9173
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             9470..10495
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             9470..10495
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10536..10710
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10788..10812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11237..12031
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13456..14044
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13945..13964
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14198..14215
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14230..14370
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14296..14315)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14456..14478
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14714..14908
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14974..16047)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16476..17105)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    18406..18430
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     18618..18640
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18632..18649
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     18633..18649
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggccaatcca gaagatggac aagtctagga ctcgagtgtc ccattcgcca tgccgaagca
       61 tgttgcccag ccggcgccag cgaggaggct gggaccatgc cggcctgaga ccaagccaga
      121 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat
      181 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca
      241 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac
      301 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga
      361 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag
      421 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
      481 ttgtggatgt acaggtctcc ccgtcaatga aagacggatt tctgacgagc ttactcgttt
      541 cgtcctcacg gactcatcag agaaatcctg caggctgcac acacaattac cgaagcatgg
      601 tcagcaacta tgaacatgaa attataacaa taccaagcaa gtacagcaga aatcagtcaa
      661 tcaaattatt ccaatatgtt agatacacga tctaaaccac aataacatca gtgtacctat
      721 caacaccaac agtgacctaa caaaatagag agtcgcaccg tgctcgagtt cacaaaaata
      781 atctgcaggt ctatgaacta ctagaattcc atcggcaaac tgattcaata actactagca
      841 ctatcccgag agcacataag gcaggttaat taacataggc tagaatttac aggtgcaaca
      901 gttgataaga taggcataag caagtaaaat atcctgaaag ctatgctatg taatcactaa
      961 tcaacaagac gcagccctta gattaaacat gaacagagta gaatctatgc aactagaggg
     1021 actgaatcaa aaacaaccta agcaaaatat acctgaagct gctacttgga tcaacgagaa
     1081 aaacagagac taaatcctac tgattgaaca gatcggaaca aatcacaacc ctagcacagt
     1141 tctacttcta gatccgaacc aataaatact tggaccacga acagcatcgc tcataatcac
     1201 agatccagca taccacgcga accccatcta accctgttca aatctaccac agatcgcaaa
     1261 acggagacta acaacaacat ctcacaatca cgaacaggcc gggaaatcaa atcaaacgcg
     1321 aggaacggag aggatcagaa aatcgcatac cttgagaagg cgattcgacg cttcgcttcg
     1381 aggggagatt tttttttctc tgcgattttg gggattcgat tgatggagga agaggcgagg
     1441 agggggtggg gtatttatag gtggggaggg ggggactcgt gagtgggccc aggggtgcgg
     1501 tgggtggggc ccagtgagaa tatagacaaa taaagagagg gagaggcggt ttggggagtc
     1561 cgtccccacc acgcaaacgc gagaaggaaa gaaaggaacg gaaagggggc gggtccagac
     1621 ggcgccgcgg agatatcgag gaggtggaaa gctgatgccg tttgctcacg gcggggttgg
     1681 tggccgttaa cacgcagcga agcggcgtta ctgccgtgta cgttttgtga cctttcgaaa
     1741 gagagcccca gcccaagaaa ggctggccag gtttttttgt actgtgtacg attacgacgg
     1801 aagaaaacat actcgttgtt ttgtattggt agatactggg ccgtagtaaa attagaatca
     1861 gttgttaatt ggttattata tataccatct taagctacca aaattaataa agttgtgatg
     1921 gctatggtat attttggtag tgaattatat tgatttggta tatattagaa tcaatccaac
     1981 cacaacacaa ccaaaaatac ctgaagctca atattgatgg tgcttttcga ctatctagca
     2041 aatgtggtga atggggcttt ataattgaag cagcccagga tgcactttgt gcagagactg
     2101 atgtgggcgc gccgtccgag ctccttatct ttaatcatat tccatagtcc ataccatagc
     2161 acatacagta gttatatgct gcagaagaga tccaacaaaa cattcacaat ggattataga
     2221 aacatttgtt tattcattat aatgtgatct tacattcatt taatattaga aaaagccaca
     2281 aattcataac acaacaagcc aagaaaaaaa cacaaactta agcacacaag ctttttattt
     2341 gacacaccaa atatttcatc ttcatcttca tatactcgag tacgcgttca cttctttttc
     2401 ttagcctgtc cggccttttt ggtggcagca ggacgcttcg atgtgagctg aagcttgatc
     2461 cgagaaccgt cagggttctc ttcatcactg gactgctcgc cgatgccctt gaccgtcagc
     2521 gcaatgttcg ccgcagcaac gtggtcggcg ttgcaaaccc agacttgctt ttgatcgaaa
     2581 actatggaca cggcgccggt tgccacttta tgcgtggcga aataaattcg cccccctcta
     2641 acaggaatgt aaaccacagc ctctttgttc cccagctttt cggccagcct gttttgcaac
     2701 acccagcacg ggatattaga tttacggtca ctacgccatt taagcaactc ctcctcaagg
     2761 tcttggagct cgtagtgact caggaactct ttcaccccct ggtgatagta ctcacctgta
     2821 ccgatgttct ttgctctaag attctgagaa agcttcctca ggacccaatc cccgatatcc
     2881 ttaaccggta tggcggccca gcggcacttc atggctttgt caggattccg gtgtaccaga
     2941 gggtcttgat gagaggtgta tagagaacca cagccgaaca aggtgatatt gtgcatgggg
     3001 gccagttgcc ggattttgtt aaagactccc ctagctaacc aatccatgga tctgctattt
     3061 gctttcttct tagtcgcatt gtttgtggtg gacaggtcgc cttctccgcg gataaacttg
     3121 atgttattct ccaggcaggt ctgaatcagg ctattggcga ttctttccac cttttgcttt
     3181 ttcttccttg ttcgtatgag ctcgagtttt tctaaaagag cgaatagctc tggatcgaac
     3241 tccttcctct gctcatctga tatcggatta tttttggacg cgttaagagc tgtagaaaaa
     3301 taggagtaga tgattccctt aaccgccttc acagtttcta acgtcgatag actcagtgag
     3361 cccaagcgag taactccaca atcctgctct ataaagcgga aaatttcttg ccttatctgc
     3421 tgcagctcga ccaaggattt tccccgaacg atatctctaa ggagatatgc gtattcctta
     3481 ttgaatttgt agaggcgtgg ctggtacttg cagatggcgt caaacttctc tttcgcttca
     3541 actgtgacaa tttccttttt cttatttttt ttggtgatct gccataggct cacgaacttg
     3601 gaagcttttt tcctccaatc ggcatactgt ggatacgcca gcccctcata tgaaagttga
     3661 tcaaattggt tccctctgtt ctccgttata gagacgatgt caccggaact tatgaaccta
     3721 acaaagcagc cgagctcctt gtggtattga ccctccttga ccacctccca taaagaataa
     3781 gcatgacttg cggtttgatt ctgatcgtag ccgcaaaacc tatcgcctat ctgtagggta
     3841 cccttttgcc ttccgacgtc gaactttaca ggtattctca cctgtccttt ggaattaatg
     3901 gtagccgaaa ttctggttat cttcaaattt gacacgggca atgtgccttg ctggatagca
     3961 agacggatgc gtgcctccgt cttagcggct ttcacgtgct tcttgtttac ccgaatcgcg
     4021 gtttggtcag tgagcttggg tagatgataa ccaaaacgcg gagtcctgaa ctggcaggta
     4081 tcgactggtg aatttcccgc ggcgtaaatc tcttggaaga agcgagtgtc ataaaatgga
     4141 atgtggtgtt tcttccatcg gccgtctgga tggcgcagct tcaggtagag ccagatacgc
     4201 agatcgaggc tattaggccg atcgttcctt tgcgcctttt ccggcagaat caccgcgttt
     4261 ctccacgtga agccctggtt gccaagtact gaggggtttg ccttctgaga cttatacctg
     4321 tcaagctgct gattatattt cgctgctgcg aggatatcct gcgcgctaca ttcctgacgt
     4381 atcgtaaaaa tgtacctaag aatattgcga atcggctcct ttttgaagtt gttcttgaga
     4441 tcatcacaga gcacaacgat ggcgttttcc ggatcagcag gatcatcctc ccacgcctta
     4501 tagagcttgg atagtctctt cccgcccaaa tgatgcacgc agatggtgta agtctcttcc
     4561 ccgctaaaaa attcgctgtc gaagaagtcg tttaatttct tgacaactag taaattgtta
     4621 aggctctgga ttctcttaaa ctgttcaagc ctctgcttgg cgaagttgta attccgagtt
     4681 gatttgatct tgagagctgt atgagccttg ttgaacatct cgccccaagc ccagaggtcg
     4741 tactgtatgg tgttctgctc cacgtaactt ctcaattcct cctgacagca ccaatctcgc
     4801 tctttcgatt taccgcggat taccttcttg agatccgttt gtaacttctt tagatcaaac
     4861 tctttctgac cgtccttcgc aatgaatctt tccagtgtac tgggggcgcc taaattgccg
     4921 gcgtagacct gccggaatgt ctcctttgtg gctttggcga cgttcagaat gatttcctga
     4981 attggggcca cattctttgg aatctctttc agattggaga tagcctccag tatctttttc
     5041 gtaatgcgca gcttcactga tcgatcctcc tttctaccgg taccaaacaa cacagagata
     5101 atactattgt actgattgct ttcagtggct actgccaagc acttctcccg gcagagaatc
     5161 tccaagtcgt gagttagatc agagacttca actccgagtt cacggcagag gtcataatac
     5221 tgctgccggc agtcgttcca gtaatacgat tctccaatgc ttgcgctgaa gtattgcttg
     5281 attgcatcag atgccgtgcc gccatagtac tcctcgaact tttcgacgag attgtcggag
     5341 gctattgtat gagtcaatga gtcctgtgac accggtctga accaatttac cgcacaccag
     5401 agtgcaatgt cttgttgttg tttctcctgc tccgtgctaa agcggacgat ttcgggcgtt
     5461 atccccccaa acaagccttg cagcatcttg aagaagaatg ctgatccgtc ctcgagacac
     5521 tgtattgtcg atttcaacag ttggttcttc cgttcgttag gtctgagcac cgacttgtag
     5581 gacttgatag ccgagctagc cgcaggcacc ccgtgaatac caaccttccg cttcttctta
     5641 ggagccatgg cggcctgcag gaagtaacac caaacaacag ggtgagcatc gacaaaagaa
     5701 acagtaccaa gcaaataaat agcgtatgaa ggcagggcta aaaaaatcca catatagctg
     5761 ctgcatatgc catcatccaa gtatatcaag atcaaaataa ttataaaaca tacttgttta
     5821 ttataataga taggtactca aggttagagc atatgaatag atgctgcata tgccatcatg
     5881 tatatgcatc agtaaaaccc acatcaacat gtatacctat cctagatcga tatttccatc
     5941 catcttaaac tcgtaactat gaagatgtat gacacacaca tacagttcca aaattaataa
     6001 atacaccagg tagtttgaaa cagtattcta ctccgatcta gaacgaatga acgaccgccc
     6061 aaccacacca catcatcaca accaagcgaa caaaaagcat ctctgtatat gcatcagtaa
     6121 aacccgcatc aacatgtata cctatcctag atcgatattt ccatccatca tcttcaattc
     6181 gtaactatga atatgtatgg cacacacata cagatccaaa attaataaat ccaccaggta
     6241 gtttgaaaca gaattaattc tactccgatc tagaacgacc gcccaaccag accacatcat
     6301 cacaaccaag acaaaaaaaa gcatgaaaag atgacccgac aaacaagtgc acggcatata
     6361 ttgaaataaa ggaaaagggc aaaccaaacc ctatgcaacg aaacaaaaaa aatcatgaaa
     6421 tcgatcccgt ctgcggaacg gctagagcca tcccaggatt ccccaaagag aaacactggc
     6481 aagttagcaa tcagaacgtg tctgacgtac aggtcgcatc cgtgtacgaa cgctagcagc
     6541 acggatctaa cacaaacacg gatctaacac aaacatgaac agaagtagaa ctaccgggcc
     6601 ctaaccatgg accggaacgc cgatctagag aaggtagaga gggggggggg ggaggacgag
     6661 cggcgtacct tgaagcggag gtgccgacgg gtggatttgg gggagatctg gttgtgtgtg
     6721 tgtgcgctcc gaacaacacg aggttgggga aagagggtgt ggagggggtg tctatttatt
     6781 acggcgggcg aggaagggaa agcgaaggag cggtgggaaa ggaatccccc gtagctgccg
     6841 gtgccgtgag aggaggagga ggccgcctgc cgtgccggct cacgtctgcc gctccgccac
     6901 gcaatttctg gatgccgaca gcggagcaag tccaacggtg gagcggaact ctcgagaggg
     6961 gtccagaggc agcgacagag atgccgtgcc gtctgcttcg cttggcccga cgcgacgctg
     7021 ctggttcgct ggttggtgtc cgttagactc gtcgacggcg tttaacaggc tggcattatc
     7081 tactcgaaac aagaaaaatg tttccttagt ttttttaatt tcttaaaggg tatttgttta
     7141 atttttagtc actttatttt attctatttt atatctaaat tattaaataa aaaaactaaa
     7201 atagagtttt agttttctta atttagaggc taaaatagaa taaaatagat gtactaaaaa
     7261 aattagtcta taaaaaccat taaccctaaa ccctaaatgg atgtactaat aaaatggatg
     7321 aagtattata taggtgaagc tatttgcaaa aaaaaaggag aacacatgca cactaaaaag
     7381 ataaaactgt agagtcctgt tgtcaaaata ctcaattgtc ctttagacca tgtctaactg
     7441 ttcatttata tgattctcta aaacactgat attattgtag tactatagat tatattattc
     7501 gtagagtaaa gtttaaatat atgtataaag atagataaac tgcacttcaa acaagtgtga
     7561 caaaaaaaat atgtggtaat tttttataac ttagacatgc aatgctcatt atctctagag
     7621 aggggcacga ccgggtcacg ctgcactgca ggcgcgccga tccccgggta ccgagctcga
     7681 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     7741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     7801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt
     7921 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
     7981 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
     8041 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
     8101 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
     8161 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
     8221 acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc tcgtctactc
     8281 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
     8341 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
     8401 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
     8461 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     8521 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     8581 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     8641 aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct
     8701 atctctctcg agctttcgca gatccggggg gcaatgagat atggtgagca agggcgagga
     8761 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     8821 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     8881 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     8941 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     9001 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     9061 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     9121 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     9181 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     9241 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     9301 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     9361 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     9421 caccggcggc atggacgagc tttacaagtc gggcggaggt ggctctgata tgaaaaagcc
     9481 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtgtccga
     9541 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg
     9601 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta
     9661 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag
     9721 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc
     9781 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc
     9841 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata
     9901 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac
     9961 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg
    10021 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt
    10081 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga
    10141 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca
    10201 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccac gactccgggc
    10261 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga
    10321 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt
    10381 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt
    10441 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaaga aatagagtag
    10501 atgccgaccg ggatctgtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag
    10561 ttcccagata agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga
    10621 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta
    10681 aaaccaaaat ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac
    10741 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
    10801 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
    10861 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
    10921 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
    10981 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
    11041 cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttg
    11101 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
    11161 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
    11221 aggaaggaaa taataaatgg ctaaaatgag aatatcaccg gaattgaaaa aactgatcga
    11281 aaaataccgc tgcgtaaaag atacggaagg aatgtctcct gctaaggtat ataagctggt
    11341 gggagaaaat gaaaacctat atttaaaaat gacggacagc cggtataaag ggaccaccta
    11401 tgatgtggaa cgggaaaagg acatgatgct atggctggaa ggaaagctgc ctgttccaaa
    11461 ggtcctgcac tttgaacggc atgatggctg gagcaatctg ctcatgagtg aggccgatgg
    11521 cgtcctttgc tcggaagagt atgaagatga acaaagccct gaaaagatta tcgagctgta
    11581 tgcggagtgc atcaggctct ttcactccat cgacatatcg gattgtccct atacgaatag
    11641 cttagacagc cgcttagccg aattggatta cttactgaat aacgatctgg ccgatgtgga
    11701 ttgcgaaaac tgggaagaag acactccatt taaagatccg cgcgagctgt atgatttttt
    11761 aaagacggaa aagcccgaag aggaacttgt cttttcccac ggcgacctgg gagacagcaa
    11821 catctttgtg aaagatggca aagtaagtgg ctttattgat cttgggagaa gcggcagggc
    11881 ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc agggaggata tcggggaaga
    11941 acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg agaaaataaa
    12001 atattatatt ttactggatg aattgtttta gtacctagaa tgcatgacca aaatccctta
    12061 acgtgagttt tcgttccact gagcgctgat gaatccccta atgattttta tcaaaatcat
    12121 taagttaagg tagatacaca tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa
    12181 tcagacaaca aaatgtgcga actcgatatt ttacacgact ctctttacca attctgcccc
    12241 gaattacact taaaacgact caacagctta acgttggctt gccacgcctt acttgactgt
    12301 aaaactctca ctcttaccga acttggccgt aacctgccaa ccaaagcgag aacaaaacat
    12361 aacatcaaac gaatcgaccg attgttaggt aatcgtcacc tccacaaaga gcgactcgct
    12421 gtataccgtt ggcatgctag ctttatctgt tcgggcaata cgatgcccat tgtacttgtt
    12481 gactggtctg atatccgtga gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta
    12541 cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc tttcagagca atgttcaaag
    12601 aaagctcatg accaatttct agccgacctt gcgagcattc taccgagtaa caccacaccg
    12661 ctcattgtca gtgatgctgg ctttaaagtg ccatggtata aatccgttga gaagctgggt
    12721 tggtactggt taagtcgagt aagaggaaaa gtacaatatg cagacctagg agcggaaaac
    12781 tggaaaccta tcagcaactt acatgatatg tcatctagtc actcaaagac tttaggctat
    12841 aagaggctga ctaaaagcaa tccaatctca tgccaaattc tattgtataa atctcgctct
    12901 aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc accacccgtc acctaaaatc
    12961 tactcagcgt cggcaaagga gccatggatt ctagcaacta acttacctgt tgaaattcga
    13021 acacccaaac aacttgttaa tatctattcg aagcgaatgc agattgaaga aaccttccga
    13081 gacttgaaaa gtcctgccta cggactaggc ctacgccata gccgaacgag cagctcagag
    13141 cgttttgata tcatgctgct aatcgccctg atgcttcaac taacatgttg gcttgcgggc
    13201 gttcatgctc agaaacaagg ttgggacaag cacttccagg ctaacacagt cagaaatcga
    13261 aacgtactct caacagttcg cttaggcatg gaagttttgc ggcattctgg ctacacaata
    13321 acaagggaag acttactcgt ggctgcaacc ctactagctc aaaatttatt cacacatggt
    13381 tacgctttgg ggaaattatg aggggatctc tcagcactga gcgtcagacc ccgtagaaaa
    13441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    13501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    13561 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
    13621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    13681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    13741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    13801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    13861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    13921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    13981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    14041 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    14101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    14161 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    14221 ggaagagggc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
    14281 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
    14341 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
    14401 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtttccggg
    14461 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
    14521 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
    14581 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
    14641 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt gggctgtgcg
    14701 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
    14761 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
    14821 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
    14881 tgggttccca atgtacgtgc tatccacagg aaagaggcct tttcgacctt tttcccctgc
    14941 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
    15001 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
    15061 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
    15121 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
    15181 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
    15241 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
    15301 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
    15361 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
    15421 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
    15481 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
    15541 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
    15601 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
    15661 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
    15721 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
    15781 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
    15841 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
    15901 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
    15961 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
    16021 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
    16081 ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
    16141 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
    16201 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
    16261 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
    16321 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    16381 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    16441 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    16501 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    16561 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    16621 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    16681 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    16741 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    16801 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    16861 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    16921 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    16981 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    17041 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    17101 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    17161 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    17221 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    17281 tttcatgcag ccgcacggtt gagccgtgcg cgggcggctc gaacacgtac ccggccgcga
    17341 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    17401 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    17461 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    17521 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    17581 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    17641 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    17701 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    17761 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    17821 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    17881 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    17941 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    18001 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    18061 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    18121 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    18181 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    18241 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    18301 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
    18361 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
    18421 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatccaagct
    18481 caagctgctc tagcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    18541 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    18601 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccacactgag
    18661 ctcgatatcg gcgcgccccg ggagctcgca aaacacacct agactagatt tgttttgcta
    18721 acccaattga tattaattat atatgattaa tatttatatg tatatggaat tggttaataa
    18781 aatgcatctg gttcatcaaa gaattataaa gacacgtgac attcatttag gataagaaat
    18841 atggatgatc tctttctctt attcagataa ttagtaatta cacataacac acaactttga
    18901 tgcccacatt atagtgatta gcatgtcact atgtgtgcat ccttttattt catacattaa
    18961 ttaactt
//
