LOCUS       sequence-476316-       14642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14642)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 14642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER11 (miR-RUG)
FEATURES             Location/Qualifiers
     source          1..14642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1984..2313)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1998..2133
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2052..2071)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2298..2318
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2327..2755)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2437..2458)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2649..2668
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2801..3025
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3014..3031
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             3132..3365
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3572..4160
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3678..3689
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4087..4107
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(4182..4895,4896..4898,4899..4901))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4185..4895,4896..4898,4899..4901))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4227..4248)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4576..4595
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4835..4856
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4919..5491
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5078..5097)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5307..5324
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(join(5540..5548,5549..5584,5585..5587,5588..5623,
                     5624..5626,5627..5662,5663..5668,5669..6286))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(6293..6326)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(6333..7544)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(6477..6496)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     ncRNA           complement(7595..7707)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(7731..7751)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      9027..9036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9046..9069)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(9070..9087)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(9108..9124)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     ncRNA           complement(9125..9218)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(9269..9964)
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     primer_bind     complement(10040..10064)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(10061..10099)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(10132..10150,10151..10173,10174..10192,10193..10215,
                     10216..10234,10235..10257,10258..10276,10277..10299,
                     10300..10318,10319..10341,10342..10360,10361..10383,
                     10384..10402)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10472..10519
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        10511..10529
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      10533..10542
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10539..10913
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10978..11095
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(11393..11437)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11622..11855
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    12352..12477
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             12524..13157
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    13325..13459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        13507..13611
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13612..13680,13681..14472)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13830..13849)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggtta gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcatcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gattagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg ctattccttt gccctcggac gagtgctggg
     1321 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     1381 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     1441 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     1501 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     1561 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     1621 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     1681 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     1741 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     1801 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1861 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtcacg tcgcggtgag
     1921 ttcaggcttt ttcatcacgt gctgatcaga tccgaaaatg gatatacaag ctcccgggag
     1981 ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     2041 ggcagaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     2101 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     2161 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     2221 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     2281 gcctggggac tttccacacc ctaactgaca cacattccac agaattaatt cgcgttaaat
     2341 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     2401 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     2461 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     2521 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     2581 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     2641 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     2701 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga
     2761 taccccctag agccccagct ggttctttcc gcctcagaag ccatagagcc caccgcatcc
     2821 ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc tgtcctgccc caccccaccc
     2881 cccagaatag aatgacacct actcagacaa tgcgatgcaa tttcctcatt ttattaggaa
     2941 aggacagtgg gagtggcacc ttccagggtc aaggaaggca cggggaaggg gcaaacaaca
     3001 gatggctggc aactagaagg cacagtcgag gctgatcagc gggtttaaac aatgtcaagg
     3061 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     3121 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     3181 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     3241 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     3301 ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc
     3361 ttccagtccc ctcttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     3421 tggtcttaaa ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc
     3481 tagccaggca caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt
     3541 ttctaggtct cgactgcaga attaattcca ggcggggagg cggcccaaag ggagatccga
     3601 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     3661 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     3721 atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc
     3781 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     3841 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     3901 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     3961 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     4021 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     4081 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     4141 ttgtaatcca gaggttgatt gttccagacg cgcttaatta attacttgta cagctcgtcc
     4201 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     4261 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     4321 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     4381 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     4441 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     4501 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     4561 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     4621 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     4681 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     4741 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     4801 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     4861 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggatcg atacatggta
     4921 ttatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta gacgtttttt
     4981 taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag atcccataca
     5041 atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg agagccattt
     5101 gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg cctttgcagg
     5161 tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg gggggttccg
     5221 ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca gaggaactgc
     5281 ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa gacccctagg
     5341 aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa aagacggcaa
     5401 tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc ggcttcggcc
     5461 agtaacgtta gggggggggg agggagaggg gcggaattgg cgcgccatac ttcgtataat
     5521 gtatgctata cgaagttatt tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt
     5581 cagcaggcag catatcaagg tcaaagtcgt caagggcatc ggctgggagc atgtctaagt
     5641 caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca ctttagctgt ttctccaggc
     5701 cacatatgat tagttccagg ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca
     5761 gctcaattgc ttgtctcaga agtgggggca tagaatcggt ggtaggtgtc tctctttcct
     5821 cttttgctac ttgatgctcc tgttcctcca atacgcagcc cagtgtaaag tggcccacgg
     5881 cggacagagc gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct
     5941 gattttccag ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc
     6001 cgtcgcgatg tgagaggaga gcacagcggt atgacttggc gttgttccgc agaaagtctt
     6061 gccatgactc gccttccagg gggcagaagt gggtatgatg cctgtccagc atctcgattg
     6121 gcagggcatc gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa
     6181 ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc gataccgaca ccattgagta
     6241 attccagagc tccgtttatg actttgctct tgtccagtct agacatggtg gcataacttc
     6301 gtatagcata cattatacga agttatggat ccgtctaaca aaaaagccaa aaacggccag
     6361 aatttagcgg acaatttact agtctaacac tgaaaattac atattgaccc aaatgattac
     6421 atttcaaaag gtgcctaaaa aacttcacaa aacacactcg ccaaccccga gcgcatagtt
     6481 caaaaccgga gcttcagcta cttaagaaga taggtacata aaaccgacca aagaaactga
     6541 cgcctcactt atccctcccc tcaccagagg tccggcgcct gtcgattcag gagagcctac
     6601 cctaggcccg aaccctgcgt cctgcgacgg agaaaagcct accacacacc taccggcagg
     6661 tggccccacc ctgcattata agccaacaga acgggtgacg tcacgacacg acgagggcgc
     6721 gcgctcccaa aggtacgggt gcactgccca acggcaccgc cataactgcc gcccccgcaa
     6781 cagacgacaa accgagttct ccagtcagtg acaaacttca cgtcagggtc cccagatggt
     6841 gccccagccc atctcacccg aataagagct ttcccgcatt agcgaaggcc tcaagacctt
     6901 gggttcttgc cgcccaccat gccccccacc ttgtttcaac gacctcacag cccgcctcac
     6961 aagcgtcttc cattcaagac tcgggaacag ccgccatttt gctgcgctcc ccccaacccc
     7021 cagttcaggg caaccttgct cgcggaccca gactacagcc cttggcggtc tctccacacg
     7081 cttccgtccc accgagcggc ccggcggcca cgaaagcccc ggccagccca gcagcccgct
     7141 actcaccaag tgacgatcac agcgatccac aaacaagaac cgcgacccaa atcccggctg
     7201 cgacggaact agctgtgcca cacccggcgc gtccttatat aatcatcggc gttcaccgcc
     7261 ccacggagat ccctccgcag aatcgccgag aagggactac ttttcctcgc ctgttccgct
     7321 ctctggaaag aaaaccagtg ccctagagtc acccaagtcc cgtcctaaaa tgtccttctg
     7381 ctgatactgg ggttctaagg ccgagtctta tgagcagcgg gccgctgtcc tgagcgtccg
     7441 ggcggaagga tcaggacgct cgctgcgccc ttcgtctgac gtggcagcgc tcgccgtgag
     7501 gaggggggcg cccgcgggag gcgccaaaac ccggcgcgga ggccagatct tacgcgtcgc
     7561 cgcgtgttta aacgcattag tcttccaatt gaaaaaagtg atttaattta taccatttta
     7621 attcagcttt gtaaaaatgt atcaaagaga tagcaaggta ttcagtttta gtaaacaaga
     7681 taattgctcc taaagtagcc ccttgaattc gtaatagatc cacggagatc accctgaaaa
     7741 tacaaattct cagaattgtg agatctaggg ataacagggt aatccgcggt cgacggtatc
     7801 ggggaccaaa atggcaagta aaatagcctg atgggatagg ctctaagtcc aacgaaccag
     7861 tgtcaccact gacacaatga ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc
     7921 gaaggcggcc attagccagg cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata
     7981 tttgggtaaa aaagggcact taacccttca gatgacgacc ctgcgtgagc tgccgccaga
     8041 agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct
     8101 gaatgcgcgt aaagcggaac tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac
     8161 gattgatgtc tctctgccag gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg
     8221 taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc
     8281 ggaaatcgaa gacgattatc ataacttcga tgctctgaac attcctggtc accacccggc
     8341 gcgcgctgac cacgacactt tctggtttga cactacccgc ctgctgcgta cccagacctc
     8401 tggcgtacag atccgcacca tgaaagccca gcagccaccg attcgtatca tcgcgcctgg
     8461 ccgtgtttat cgtaacgact acgaccagac tcacacgccg atgttccatc agatggaagg
     8521 tctgattgtt gataccaaca tcagctttac caacctgaaa ggcacgctgc acgacttcct
     8581 gcgtaacttc tttgaggaag atttgcagat tcgcttccgt ccttcctact tcccgtttac
     8641 cgaaccttct gcagaagtgg acgtcatggg taaaaacggt aaatggctgg aagtgctggg
     8701 ctgcgggatg gtgcatccga acgtgttgcg taacgttggc atcgacccgg aagtttactc
     8761 tggtttcggc ttcgggatgg ggatggagcg tctgactatg ttgcgttacg gcgtcaccga
     8821 cctgcgttca ttcttcgaaa acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa
     8881 tagattatga aattcagtga actgtggtta cgcgaatggg tgaacccggc gattgatagc
     8941 gatgcgctgg caaatcaaat cactatggcg ggcctggaag ttgggggatc gatccggtac
     9001 cattaccctg ttatccctat ctagacccat ggtggcgtct ccttcgggcc cctggaagag
     9061 aacttccaga cttccacggg gtaccagcat atggacgtca agcttatcga taccgtcgac
     9121 ctcgagcctt ctgttgggtt aacctgaaga agtaatccca gcaagtgttt ccaagatgtg
     9181 caggcaacga ttctgtaaag tactgaagcc tcattcaaca tagtatatgt gctgccgaag
     9241 cgagcactta acaaggcttg cggccgcatc atctgtgccc cagtttgcta gggaggtcgc
     9301 agtacttggc cacagccatc tcgtgctgct cgacgtaggt ctctttgtcg gcctccttga
     9361 ttctttccag tctgtggtcc acgaagtgga agccgggcat cttgaggttc ttagcgggtt
     9421 tcttggatct gtatgtggtc ttgaaggagc agtgcaggta gcccccgccc acgagcttca
     9481 gggccatctg gctgtggcct ctcaggccgc cgtcagcggg gtacagcatc tcggtgttgg
     9541 cctcccagcc gcgtgttttc ttctgcatca cagggccgtt ggatgggaag ttcaccccgt
     9601 tgatcttgac gttgtagatg atgcagccgt tctggaagct ggtgtcctgg gtagcggtca
     9661 gcacgccccc gtcttcgtat gtggtgattc tctcccatgt gaagccctca gggaaggact
     9721 gcttaaagaa gtcggggatg ccctgggtgt ggttgatgaa ggctttgctg ccgtacatga
     9781 agctggtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgatct
     9841 tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtggt
     9901 ggttgttcac ggtgccctcc atgtacagct tcatgtgcat gttctccttg atcagctcgc
     9961 tcatggtggc gaccggtagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc
    10021 gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt atataggcct
    10081 cccaccgtac acgcctacct cgacccgggt accgagctcg actttcactt ttctctatca
    10141 ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa
    10201 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
    10261 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
    10321 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
    10381 ttttctctat cactgatagg gagtggtaaa ggatccagtc gactgaattg gttcctttaa
    10441 gctagcctag aacctcggac ctgcagcacg tgttgacaat taatcatcgg catagtatat
    10501 cggcatagta taatacgact cactatagga gggccaccat ggccaagttg accagtgccg
    10561 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
    10621 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
    10681 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
    10741 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
    10801 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
    10861 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgattccgga
    10921 ctagagcggg cccggtacta accaaactgg atctctgctg tccctgtaat aaacccgaaa
    10981 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
    11041 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
    11101 gatgaatact gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc
    11161 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
    11221 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
    11281 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
    11341 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
    11401 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
    11461 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
    11521 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
    11581 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
    11641 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
    11701 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
    11761 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
    11821 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
    11881 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
    11941 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
    12001 tcagcggccg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
    12061 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
    12121 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
    12181 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
    12241 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
    12301 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
    12361 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
    12421 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
    12481 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
    12541 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
    12601 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
    12661 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
    12721 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
    12781 ccctggaaag tccccagcgg aaagtccctt gtagcaagct cgatatcagc agttcttgaa
    12841 gtactccgga tgcagctctc gggccacgtg atgaaatgct aggcggctgt caaacctcca
    12901 ctctaacact tctctctccg ggtcatccat cccatgcagg ctcacagggt gtaacaagct
    12961 ggtgttctct cctttattgg cctcttctac cttatctggc tcaactggta ctagcttgta
    13021 gcaccatcca aaggtcagtg gatatctgac ccctggccct ggtgtgtagt tctgctaatc
    13081 agggaagtag ccttgtgtgt ggtagatcca cagatcaagg atatcttgtc ttctttggga
    13141 gtgaattagc ccttccaact actaagtttg tagtacatat ttaacaaata caatttcttt
    13201 aaaatgaaaa taattcagag gaatcacagg tttagagtaa atgaaaccac aggtaattgg
    13261 cagtggtaat agggtatggg gtgggaagtt tgggatgatt ttggttagct tgagttatcc
    13321 agttgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
    13381 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
    13441 aagctgcaat aaacaagttg atctcccgat ccgtcgacgt caggtggcac ttttcgggga
    13501 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    13561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    13621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    13681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    13741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    13801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    13861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    13921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    13981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    14041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    14101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    14161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    14221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    14281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    14341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    14401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    14461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    14521 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    14581 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    14641 tc
//
