LOCUS       sequence-476318-        8367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, 
            Conaway JW, Conaway RC
  TITLE     Subunit composition and substrate specificity of a MOF-containing 
            histone acetyltransferase distinct from the male-specific lethal 
            (MSL) complex.
  JOURNAL   J Biol Chem. 2010 Feb 12. 285(7):4268-72.
  PUBMED    20018852
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2010 Feb 12. 285(7):4268-72."
COMMENT     Sequence Label: pcDNA5FRT/Flag-KIAA1267
FEATURES             Location/Qualifiers
     source          1..8367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        514..717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     668..688
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     762..781
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        762..780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             806..829
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4218..4235)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4224..4448
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4581..4600)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    4732..4779
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4787..5809
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             4787..5809
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    5939..6060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5976..5995)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6030..6049
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6109..6125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6109..6125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6122..6144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6133..6149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6157..6163,6164..6181,6182..6187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6202..6223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6340..6357)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6511..7099)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6591..6610)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7270..8061,8062..8130))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7893..7912
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8131..8235)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8310..8329)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
       61 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
      121 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
      721 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
      781 gagacccaag ctggctagca tgccagacta caaggacgat gatgacaagc tcgagatggc
      841 tgcgatggcg cccgctctca ctgacgcagc agctgaagca caccatatcc ggttcaaact
      901 ggctccccca tcctctacct tgtcccctgg cagtgccgaa aataacggca acgccaacat
      961 ccttattgct gccaacggaa ccaaaagaaa agccattgct gcagaggatc ccagcctaga
     1021 tttccgaaat aatcctacca aggaagactt gggaaagctg caaccactgg tggcatctta
     1081 tctctgctct gatgtaacat ctgttccctc aaaggagtct ttgaagttgc aaggggtctt
     1141 cagcaagcag acagtcctta aatctcatcc tctcttatct cagtcctatg aactccgagc
     1201 tgagctgttg gggagacagc cagttttgga gttttcctta gaaaatctta gaaccatgaa
     1261 tacgagtggt cagacagctc tgccacaagc acctgtaaat gggttggcta agaaattgac
     1321 taaaagttca acacattctg atcatgacaa ttccacttcc ctcaatgggg gaaaacgggc
     1381 tctcacttca tctgctcttc atgggggtga aatgggagga tctgaatctg gggacttgaa
     1441 ggggggtatg accaattgca ctcttccaca tagaagcctt gatgtagaac acacaacttt
     1501 gtatagcaat aatagcactg caaacaaatc ctctgtcaat tccatggaac agccggcact
     1561 tcaaggaagc agtagattat cacctggtac agactccagc tctaacttgg ggggtgtcaa
     1621 attggagggt aaaaagtctc ccctgtcttc cattcttttc agtgctttag attctgacac
     1681 aaggataaca gctttactgc ggcgacaggc tgacattgag agccgtgccc gcagattaca
     1741 aaagcgctta caggttgtac aagccaagca ggttgagagg catatacaac atcagctggg
     1801 tggatttttg gagaagactt tgagcaaact gccaaacttg gaatccttga gaccacggag
     1861 ccagttgatg ctgactcgaa aggctgaagc tgccttgaga aaagctgcca gtgagaccac
     1921 cacttcagag ggacttagca actttctgaa aagcaattca atttcagaag aattggagag
     1981 atttacagct agtggcatag ccaacttgag gtgcagtgaa caggcatttg attcagatgt
     2041 cactgacagt agttcaggag gggagtctga tattgaagag gaagaactga ccagagctga
     2101 tcccgagcag cgtcatgtac ccctgagacg caggtcagaa tggaaatggg ctgcagaccg
     2161 ggcagctatt gtcagccgct ggaactggct tcaggctcat gtttctgact tggaatatcg
     2221 aattcgtcag caaacagaca tttacaaaca gatacgtgct aataaggggt tgatagttct
     2281 tggggaggta cctcccccag agcatacaac agacttattt cttccactta gttctgaggt
     2341 gaagacagat catgggactg ataaattgat tgagtctgtt tctcagccat tggaaaacca
     2401 tggtgcccct attattggtc atatttcaga gtcactgtct accaaatcat gtggagcact
     2461 cagacctgtc aatggagtta ttaacactct tcagcctgtc ttggcagacc acattccagg
     2521 tgacagctct gatgctgagg aacaattaca taagaagcaa cgactgaatc tcgtctcttc
     2581 atcatctgat ggcacctgtg tggcagcccg gacacgtcct gtactgagct gtaagaagcg
     2641 gaggcttgtt cgacccaaca gcatcgttcc tctttccaag aaggttcacc ggaacagcac
     2701 aatccgccct ggctgtgatg tgaatccctc ctgcgcactg tgtggttcag gcagcatcaa
     2761 caccatgcct cccgaaattc actatgaagc ccctctgttg gaacgtcttt cccagttgga
     2821 ctcttgtgtt catcctgttc tagcatttcc agatgatgtt cccacaagcc tgcatttcca
     2881 gagcatgctg aaatctcagt ggcagaacaa gccttttgac aaaatcaaac ctcccaaaaa
     2941 gttatcgctt aagcacagag cacccatgcc gggcagtctg ccagattcag ctcgtaagga
     3001 caggcacaaa ttggtcagct ccttcctaac aacagccaag ctgtcccatc accaaacccg
     3061 gcctgacagg acccacaggc agcacttaga cgatgtgggg gccgtgccca tggtggagcg
     3121 agtgacagcg ccaaaagcag agcgcttgct caacccacca ccacccgtgc atgacccaaa
     3181 ccacagcaaa atgagattgc gagaccattc atctgagaga agtgaagtgt tgaagcatca
     3241 cacagacatg agcagttcga gctacttggc agccacccac catcctccac acagtccctt
     3301 ggtgcgacag ctctccacct cctcagattc ccctgcaccc gccagctcta gctcacaggt
     3361 tacagccagc acatcgcagc agccagtaag gaggagaagg ggagagagct catttgatat
     3421 taacaacatt gtcatcccaa tgtctgttgc tgcaacaact cgcgtagaga aactgcaata
     3481 caaggaaatc cttacgccca gctggcggga ggttgatctt cagtctctga aggggagtcc
     3541 tgatgaggag aatgaagaga ttgaggacct atccgacgca gccttcgccg ccctgcatgc
     3601 caaatgtgag gagatggaga gggcacggtg gctgtggacc acgagtgtgc caccccagcg
     3661 gcggggcagc aggtcctaca ggtcatcaga cggccggaca accccccagc tgggcagtgc
     3721 caacccctcc accccccagc ctgcctcccc tgatgtcagc agtagccact ctttgtcaga
     3781 atactcccat ggtcagtccc ctaggagccc cattagcccg gaactgcact cagcacccct
     3841 cacccctgtg gctcgggaca ctccgcgaca cttagccagt gaggataccc gttgttccac
     3901 accagagctg gggctggatg aacagtctgt ccagccctgg gaacggcgga ccttccccct
     3961 ggcgcacagt ccccaggcgg agtgtgagga ccagctggat gcacaggagc gagcagcccg
     4021 ctgcactcga cgcacctcag gcagcaagac tggccgggag acagaggcag cgcccacctc
     4081 gcctcccatt gtccccctca agagtcggca tctggtggca gcagccacag ctcagcgccc
     4141 gactcacaga tgagatatcc agcacagtgg cggccgctcg atcgagtcta gagggcccgt
     4201 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4261 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4321 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4381 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4441 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4501 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4561 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4621 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4681 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct
     4741 attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact
     4801 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     4861 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     4921 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     4981 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     5041 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     5101 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     5161 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     5221 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     5281 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     5341 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     5401 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     5461 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     5521 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     5581 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     5641 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     5701 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     5761 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg
     5821 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5881 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     5941 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6001 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6061 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     6121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6241 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6301 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6361 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6421 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6481 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6541 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6601 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6661 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6721 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6781 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6841 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6901 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6961 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7021 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7081 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7141 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7201 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7261 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7321 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7381 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7441 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7501 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7561 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7621 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7681 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7741 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7801 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7861 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7921 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7981 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8041 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8101 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8161 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8221 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     8281 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8341 cagtatctgc tccctgcttg tgtgttg
//
